Microsatellites Based Genetic Linkage Map of the &lt;i&gt;Rht3&lt;/i&gt; Locus in Bread Wheat

Navarro, Christopher; Yang, Yang; Mohan, Amita; Grant, Nathan P; Gill, Kulvinder S.; Sandhu, Devinder

doi:10.5376/mpb.2014.05.0008

Cited by 3 publications

(2 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The F 2 population had a height distribution skewed towards the parent Icaro ( Figure 1 ). The skewed distribution towards reduced height parents was also reported in the Rht3 F 2 mapping population [ 22 ]. This distribution suggests that the mutant phenotype is dominant, as only a few plants had the tall phenotype.…”

Section: Resultssupporting

confidence: 57%

Inheritance and Genetic Mapping of the Reduced Height (Rht18) Gene in Wheat

Grant

Mohan

Sandhu

et al. 2018

Plants

Self Cite

View full text Add to dashboard Cite

Short-statured plants revolutionized agriculture during the 1960s due to their ability to resist lodging, increased their response to fertilizers, and improved partitioning of assimilates which led to yield gains. Of more than 21 reduced-height (Rht) genes reported in wheat, only three—Rht-B1b, Rht-D1b, and Rht8—were extensively used in wheat breeding programs. The remaining reduced height mutants have not been utilized in breeding programs due to the lack of characterization. In the present study, we determined the inheritance of Rht18 and developed a genetic linkage map of the region containing Rht18. The height distribution of the F2 population was skewed towards the mutant parent, indicating that the dwarf allele (Rht18) is semi-dominant over the tall allele (rht18). Rht18 was mapped on chromosome 6A between markers barc146 and cfd190 with a genetic distance of 26.2 and 17.3 cM, respectively. In addition to plant height, agronomically important traits, like awns and tiller numbers, were also studied in the bi-parental population. Although the average tiller number was very similar in both parents, the F2 population displayed a normal distribution for tiller number with the majority of plants having phenotype similar to the parents. Transgressive segregation was observed for plant height and tiller number in F2 population. This study enabled us to select a semi-dwarf line with superior agronomic characteristics that could be utilized in a breeding program. The identification of SSRs associated with Rht18 may improve breeders’ effectiveness in selecting desired semi-dwarf lines for developing new wheat cultivars.

show abstract

Section: Resultssupporting

confidence: 57%

Inheritance and Genetic Mapping of the Reduced Height (Rht18) Gene in Wheat

Grant

Mohan

Sandhu

et al. 2018

Plants

Self Cite

View full text Add to dashboard Cite

show abstract

“…The Rht-B1c variant exhibited insensitivity to GA 3 which caused reduction in alphaamylase activity in seeds, increased ABA responsiveness and increased Hagberg falling number, resulting in improved resistance to sprouting damage (Flintham et al 1997;Gooding et al 2012). However, the location of Rht-B1c allele at wmc125 on chromosome 4B (Navarro et al 2014) is distant from the QPhs.spa-4B QTL.…”

Section: Discussionmentioning

confidence: 77%

Maximizing the identification of QTL for pre-harvest sprouting resistance using seed dormancy measures in a white-grained hexaploid wheat population

et al. 2015

View full text Add to dashboard Cite

Pre-harvest sprouting in spring wheat causes significant financial loss to growers throughout the world and sprouting damage can be reduced by growing resistant genotypes. Several genetic factors, especially those related to seed dormancy, are involved in the control of pre-harvest sprouting resistance. The objective of this study was to identify quantitative trait loci (QTL) influencing pre-harvest sprouting resistance from multiple measures of dormancy at multiple germination intervals on seed harvested across multiple environments. A doubled haploid mapping population of 91 individuals derived from a cross of two Canadian white-seeded spring wheat genotypes, SC8021-V2 (pre-harvest sprouting resistant) and AC Karma (moderately susceptible to pre-harvest sprouting) was used for QTL mapping. Daily germination counts were analysed using germination index, germination resistance and percent germination at intervals of 3, 5, 7, 10, 14 and 21 days from spike samples collected from six field and one greenhouse environments in Saskatchewan, Canada. Continuous frequency distributions at certain measure-durations indicated genetic complexity of dormancy segregation in the SC8021-V2/AC Karma cross. Composite interval mapping detected significant (p B 0.05) QTL associated with resistance to pre-harvest sprouting on all 21 wheat chromosomes. Of the 26 total QTL, six were novel and the rest were detected either at the same marker or overlapping a marker interval reported in other studies. QTL expressed consistently for germination index, germination resistance and percent germination at different germination durations on chromosomes 2B, 4A, 5D and 6D. QTL identified on homoeologous chromosomes 4A, 4B and 4D with chromosome specific molecular variants of SSR marker wmc617 suggest a conserved region for controlling dormancy on group four. The majority of QTL mapped in regions known to contain factors affecting different components of pre-harvest sprouting resistance like seed dormancy, seed coat colour, ABA responsiveness and alpha-amylase activity. This study demonstrated that using multiple measures of seed dormancy Electronic supplementary material The online version of this article (doi:10.1007/s10681-015-1460-x) contains supplementary material, which is available to authorized users. Euphytica (2015) 205:287-309 DOI 10.1007/s10681-015-1460 at multiple intervals of germination enhanced identification of QTL affecting dormancy in white-seeded hexaploid wheat.

show abstract