Microsatellites and multiplex PCRs for assessing aquaculture practices of the grooved carpet shell Ruditapes decussatus in Spain

Borrell, Yaisel J.; Arias‐Pérez, Alberto; Freire, Ruth; Valdés, Antonio; Sánchez, José A.; Méndez, Josefina; Martı́nez, D.; López, J. Albéniz; Carleos, Carlos; Blanco, Gloria; Insua, Ana

doi:10.1016/j.aquaculture.2014.01.010

Cited by 30 publications

(35 citation statements)

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“…The observed significant negative values of Tajima's D and Fu's F s could be interpreted as signs of a selection regime associated with changing environmental variables and humaninduced disturbances, although they could also be derived from recent population expansion (for example, after a bottleneck). For the genetic differentiation between the hatchery stock and the wild populations, the present results are concordant with the findings of Borrell et al (2014) using microsatellites as genetic markers. Pairwise F ST values showed significant genetic differentiation between the cultured seeds intended for supplementation and the wild populations of Ría de Villaviciosa and Ría del Eo.…”

Section: Discussionsupporting

confidence: 89%

“…On the other hand, it is possible that collection of hatchery broodstock only from Ría del Eo has contributed, at least partially, to the genetic homogenization among the wild populations. Using microsatellites, Borrell et al (2014) did not find genetic differentiation between the 2 Asturian estuaries, and our results based on mitochondrial mtCOI fully support such a lack of divergence between spatially disjunct populations. It may indicate a common past for the species in those estuaries and/or contemporary gene flow through natural larval dispersal or anthropogenic activities such as the enhancement program.…”

Section: Discussionsupporting

confidence: 59%

“…It is a native species with a natural distribution in the eastern Atlantic from the British Isles to Senegal and into the Mediterranean Sea (Gofas et al 2011, Juanes et al 2012, Borrell et al 2014). This species is quite variable in shape and color as well as in shell sculpture, ranging from practically smooth to moderately reticulate (Poppe & Goto 2000).…”

Section: Introductionmentioning

confidence: 99%

“…), although the recommended number is at least 50 for short-term fitness and 500 for long-term survival (Tave 1999, Allendorf & Luikart 2007. This program has been recently assessed employing microsatellite DNA loci (Borrell et al 2014). It has been reported that the seeds employed in the enhancement program were genetically different compared to their wild counterparts, and that the wild populations located in the central Cantabrian coast did not differ significantly in their microsatellite allele frequencies.…”

Section: Introductionmentioning

confidence: 99%

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Impacts of supplementation aquaculture on the genetic diversity of wild Ruditapes decussatus from northern Spain

Habtemariam¹,

Arias²,

García‐Vázquez³

et al. 2015

Aquacult. Environ. Interact.

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The European carpet shell Ruditapes decussatus is among the most important shellfish species cultured in estuaries and bays of the northern Iberian Peninsula. Increased demand and overfishing of native R. decussatus, as well as irregular yields due in part to pollution and urban sprawl, have led to overexploitation of the species and to the import of the non-indigenous R. philippinarum in the early 1980s. In recent years, supplementation aquaculture of R. decussatus (supportive breeding) has been implemented as a management tool to recover the exhausted native carpet shell populations in Asturias, northern Spain. In this study, taxonomic analysis and a suite of genetic markers (ITS2, 5SrDNA, and mtCOI) were employed to assess the genetic composition of R. decussatus in 2 estuaries of the region, Ría del Eo and Ría de Villaviciosa. Our results revealed species misidentification and evidenced genetic introgression from the non-indigenous R. philippinarum into wild R. decussatus samples (4.2%), as well as in the aquaculture seed used for population supplementation (2.0%). Furthermore, we found significant genetic differentiation (F ST = 0.03750, p < 0.0001) between wild populations and aquaculture seed, while the adults of both estuaries were genetically homogeneous (p > 0.05). Several actions are strongly recommended to prevent inadvertent introduction of non-indigenous genomes and other genetic erosive processes on native species in the region: (1) banning releases of the alien species R. philippinarum, (2) developing diagnostic tests for detection of hybrids in captive breeding programs, and (3) assessing the conservation status of wild R. decussatus populations.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 59%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Impacts of supplementation aquaculture on the genetic diversity of wild Ruditapes decussatus from northern Spain

Habtemariam¹,

Arias²,

García‐Vázquez³

et al. 2015

Aquacult. Environ. Interact.

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“…However, the expansion of Manila clam in Europe has been mediated since the beginning by hatchery spat supply. Since the effective population size of hatchery populations is usually much lower than that of wild populations4546, a reduction in genetic variability in the initial population established in Europe would be expected. Furthermore, the continued input of hatchery spat in the exploited populations could have precluded the recovery of the high genetic variability levels203233343536373842 typical of wild populations of this species.…”

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confidence: 99%

Population genetics of the Manila clam (Ruditapes philippinarum) introduced in North America and Europe

Cordero

Delgado

Liu

et al. 2017

Sci Rep

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Globally, the Manila clam (Ruditapes philippinarum) stands as the second most important bivalve species in fisheries and aquaculture. Native to the Pacific coast of Asia, it is now well-established in North America and Europe, where its on-going management reflects local economic interests. The historic record of transfers spans the 20th century and suggests sequential movement from Japan to North America, as a hitch-hiker on oysters, and then intentional introduction in Europe, but global genetic data are missing. We have studied mitochondrial DNA and microsatellite markers in nine populations from Asia, North America and Europe. The results from the two types of markers indicated a good concordance of present-day genetic structure with the reported history of clam transfers across continents, and no evidence of relevant concealed introductions from continental Asia in Europe and North America. However, European populations showed a loss of genetic variability and significant genetic differentiation as compared to their American counterparts. Our study shows that in spite of the increasing ease for species to spread out of their native range, in the case of the Manila clam this has not resulted in new invasion waves in the two studied continents.

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Mitochondrial DNA analyses of Donax trunculus (Mollusca: Bivalvia) population structure in the Iberian Peninsula, a bivalve with high commercial importance

Fernández-Pérez

Nantón

Arias‐Pérez

et al. 2018

Aquatic Conservation

Self Cite

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1. The wedge clam, Donax trunculus (Linnaeus, 1758), is an ecologically and economically important species found in the warm waters of the Atlantic and Mediterranean areas. Donax trunculus has been suffering from a severe population decline in some wild stocks of the Iberian Peninsula, and genetic monitoring of wild populations can provide valuable information to support conservation measures and management policies.2. In this study, mitochondrial 16S rDNA (16S) and cytochrome b (Cytb) gene variation was examined at 18 localities across the Iberian Peninsula to test whether the patterns of genetic diversity, population structure, and demographic inferences previously deduced by using microsatellites are supported by these molecular markers. 3. Samples (N = 557) were collected from 18 localities of the Iberian Peninsula and attention was paid to the north-western Atlantic area, where wild stocks have diminished greatly. Analysis of molecular variance, fixation index ( F ST ), and demographic inferences were estimated based on mitochondrial DNA. Since D. trunculus shows doubly uniparental inheritance, both types of mitochondrial DNA (F and M) were analysed. 4. A high level of genetic diversity was detected using the haplotype diversity dataset, showing different geographic patterns. 5. F ST values for the M type revealed significant differentiation between populations on both sides of the Almeria-Oran front, and between the locality from the Alboran Sea (FU) and localities from the Atlantic Ocean. However, the F type did not detect the differentiation of FU. This makes the M genome sequences more suitable for investigating genetic structure of D. trunculus populations. 6. Management and recovering strategies of this important resource should consider the genetic structure observed. 7. Several strategies for conservation and management of this commercial and ecologically important bivalve are proposed. * Both authors contributed equally to this work.

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Microsatellites and multiplex PCRs for assessing aquaculture practices of the grooved carpet shell Ruditapes decussatus in Spain

Cited by 30 publications

References 53 publications

Impacts of supplementation aquaculture on the genetic diversity of wild Ruditapes decussatus from northern Spain

Impacts of supplementation aquaculture on the genetic diversity of wild Ruditapes decussatus from northern Spain

Population genetics of the Manila clam (Ruditapes philippinarum) introduced in North America and Europe

Mitochondrial DNA analyses of Donax trunculus (Mollusca: Bivalvia) population structure in the Iberian Peninsula, a bivalve with high commercial importance

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