Microsatellite Variation Within Three Populations of Inbred C57BL/6J Strain

Niu, Yulan; Liang, Shu-long

doi:10.3724/sp.j.1141.2008.06599

Cited by 3 publications

(1 citation statement)

References 14 publications

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“…Microsatellite loci are typically highly polymorphic, displaying neutral, co-dominant inheritance. Recently, microsatellites have been applied in the assessment of genetic diversity and differentiation in inbred strains of animals (Niu and Hu, 2008). In this study, we isolated and characterized 16 microsatellite loci from Chinese hamster genomes.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of 16 novel microsatellite loci in two inbred strains of the Chinese hamster (Cricetulus griseus)

Song¹,

Geng²,

Jia³

et al. 2011

Genet. Mol. Res.

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ABSTRACT. The Shanyi inbred A and E strains of the Chinese hamster are widely used in biomedical research, but detailed genetic characterization has been lacking. We developed microsatellite markers that could be used for genetic diversity analysis and linkage map construction. We isolated and characterized 16 novel microsatellite loci from a microsatellite-enriched genomic DNA library. These loci were genotyped in 48 animals from the two strains, and the polymorphic information content was determined. In the Shanyi A and E populations, 14 and 15 loci were found to be polymorphic, respectively, with polymorphic information content ranging from 0.1393 to 0.8082 and from 0.1109 to 0.7397, respectively. A total of 115 alleles were found for the 16 microsatellite loci in the two populations; the mean observed heterozygosity (H O ) was 0.5191 and 0.4333 for the A and E populations, respectively, indicating marked genetic variation within the two populations. Correspondingly, the F ST values ranged from 0.002 to 0.9253, with an overall mean of 0.1935, indicating significant genetic difference between the two strains. The population differentiation levels were substantiated by Nei's genetic distance and full Bayesian analyses computed with STRUCTURE. Despite the genetic diversity and differentiation within and between the two inbred populations, the 48 individuals were correctly allocated into their original populations with high statistical confidence based on these 16 microsatellite loci. These novel microsatellite loci should be useful genetic markers for these two Chinese hamster inbred strains.

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Section: Introductionmentioning

confidence: 99%

Isolation and characterization of 16 novel microsatellite loci in two inbred strains of the Chinese hamster (Cricetulus griseus)

Song¹,

Geng²,

Jia³

et al. 2011

Genet. Mol. Res.

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Unexpected gender difference in sensitivity to the acute toxicity of dioxin in mice

Pohjanvirta

Miettinen

Sankari

et al. 2012

Toxicology and Applied Pharmacology

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Establishing references for gene expression analyses by RT-qPCR in Theobroma cacao tissues

Pinheiro¹,

Litholdo²,

Sereno³

et al. 2011

Genet. Mol. Res.

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ABSTRACT. Lack of continuous progress in Theobroma cacao (Malvaceae) breeding, especially associated with seed quality traits, requires more efficient selection methods based on genomic information. Reverse transcript quantitative PCR (RT-qPCR) has become the method of choice for gene expression analysis, but relative expression analysis requires various reference genes, which must be stable across various biological conditions. We sought suitable reference genes for various tissues of cacao, especially developing seeds. Ten potential reference genes were analyzed for stability at various stages of embryo development, leaves, stems, roots, flowers, and pod epicarp; seven of them were also evaluated in shoot tips treated either with hormones (salicylate; ethefon; methyl-jasmonate) or after inoculation with the fungus Moniliophthora perniciosa (Marasmiaceae sensu lato). For developing embryos, the three most stable genes were actin (ACT), polyubiquitin (PUB), and ribosomal protein L35 (Rpl35). In the analyses of various tissues, the most stable genes were malate dehydrogenase (MDH), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), and acyl-carrier protein B (ACP B). GAPDH, MDH and tubulin (TUB) were the most appropriate for normalization when shoot apexes were treated with hormones, while ACT, TUB and Rpl35 were the most appropriate after inoculation with M. perniciosa. We conclude that for each plant system and biological or ontogenetical condition, there is a need to define suitable reference genes. This is the first report to define reference genes for expression studies in cacao.

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Microsatellite Variation Within Three Populations of Inbred C57BL/6J Strain

Cited by 3 publications

References 14 publications

Isolation and characterization of 16 novel microsatellite loci in two inbred strains of the Chinese hamster (Cricetulus griseus)

Isolation and characterization of 16 novel microsatellite loci in two inbred strains of the Chinese hamster (Cricetulus griseus)

Unexpected gender difference in sensitivity to the acute toxicity of dioxin in mice

Establishing references for gene expression analyses by RT-qPCR in Theobroma cacao tissues

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