Microsatellite variation in the freshwater snail <i>Biomphalaria pfeifferi</i>

Charbonnel, Nathalie; Angers, Bernard; Razatavonjizay, R.; Brémond, Philippe; Jarne, Philippe

doi:10.1046/j.1365-294x.2000.00939-9.x

Cited by 18 publications

(22 citation statements)

References 4 publications

(3 reference statements)

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“…Direct selection on microsatellite loci, especially dinucleotides or tetranucleotides, is unlikely (Goldstein and Schlötterer 1999), but indirect selection as a consequence of linkage of Bpf1 with a fitness trait appears possible. Our study confirmed that B. pfeifferi is a highly selfing species (Charbonnel et al 2000;Charbonnel et al 2003). Very high selfing rates markedly reduce recombination and therefore allow for selection through hitchhiking at a very large genomic scale.…”

Section: Selectionsupporting

confidence: 80%

“…This hermaphroditic snail mainly self-fertilizes (Jelnes 1980;Charbonnel et al 2000Charbonnel et al , 2002. Demographic surveys have revealed that reproduction is continuous over the year.…”

Section: Species Studied and Samplingmentioning

confidence: 99%

“…The genetic variation was scored at six microsatellite loci developed by Charbonnel et al (2000;Bpf1, Bpf2, Bpf9, Bpf10, and Bpf12) and Jones et al (2000;Bgm16), according to Charbonnel et al (2002). We have two comments about sampling and its possible interference with the analysis of results.…”

Section: Species Studied and Samplingmentioning

confidence: 99%

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A Spatial and Temporal Approach to Microevolutionary Forces Affecting Population Biology in the Freshwater SnailBiomphalaria pfeifferi

Charbonnel

Quesnoit

Razatavonjizay³

et al. 2002

The American Naturalist

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The limitations of both population demography and genetics highlight the need to combine these approaches when inferring the influence of demographic processes and modes of migration on genetic structure. The aim of this study was to use spatiotemporal genetic and demographic surveys to reveal the microevolutionary forces acting on the metapopulation dynamics of the freshwater snail Biomphalaria pfeifferi. We also analyzed the consequences of population turnover on temporal genetic differentiation, an aspect that has been little explored. Genetic drift was revealed by both the demographic survey, which indicated severe bottlenecks or extinction during the rainy (resp. dry) season in open (resp. closed) habitats, and the genetic approach, which indicated high selfing rates and strong temporal differentiation. Genetic reassignments and temporal differentiation both confirmed the results of the demographic survey, which suggests that migration occurs in closed (resp. open) habitats during the hot and dry (resp. rainy) season, and indicated that source-sink functioning may be envisaged. A propagule pool mode of colonization was inferred in the open habitats during the rainy season and a migrant pool in the closed habitats during the dry season. Our study also suggests that selection might be inferred from patterns of neutral genetic markers when recombination is limited.

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Section: Selectionsupporting

confidence: 80%

Section: Species Studied and Samplingmentioning

confidence: 99%

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A Spatial and Temporal Approach to Microevolutionary Forces Affecting Population Biology in the Freshwater SnailBiomphalaria pfeifferi

Charbonnel

Quesnoit

Razatavonjizay³

et al. 2002

The American Naturalist

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“…Variability was analysed at nine polymorphic microsatellite loci. Seven loci were obtained from Charbonnel et al. (2000); two with perfect (Fr‐1 and Fr‐10), three with imperfect (Fr‐2, Fr‐5 and Fr‐12) dinucleotide repeat motifs and two with perfect tetranucleotide repeats (Fr‐8 and Fr‐9).…”

Section: Methodsmentioning

confidence: 99%

“…1991). Annealing temperatures ranged from 55 to 45 °C for the seven Fr loci using conditions from Charbonnel et al. (2000) as a guideline, to 65–55 °C for u‐2 and u‐7 loci, respectively.…”

Section: Methodsmentioning

confidence: 99%

Low genetic diversity in a snail intermediate host (Biomphalaria pfeifferi Krass, 1848) and schistosomiasis transmission in the Senegal River Basin

et al. 2009

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Population genetic perturbations of intermediate hosts, often a consequence of human pressure on environmental resources, can precipitate unexpectedly severe disease outbreaks. Such disturbances are set to become increasingly common following range changes concomitant with climate shifts, dwindling natural resources and major infrastructure changes such as hydroprojects. Construction of the Diama dam in the Senegal River Basin (SRB) reduced river salinity, enabling the freshwater snail intermediate host Biomphalaria pfeifferi to rapidly expand its distribution. A serious public health problem ensued, with an epidemic of intestinal schistosomiasis occurring in the previously schistosome-free Richard-Toll region within 2 years. The current study aimed to assess the population variability of B. pfeifferi in the SRB, and speculate upon its subsequent impact on host-parasite interactions following such engineered ecological change. Genetic variation at nine polymorphic microsatellite loci revealed little population differentiation in SRB snails compared with those from natural habitats in Zimbabwe, where Schistosoma mansoni transmission is much lower. 'Open' SRB habitats are associated with greater water contact, smaller population sizes and less genetic diversity, with sites downstream of Richard-Toll showing greater inter- and intrapopulation variation, concomitant with less frequent human contact. These observations may be explained by rapid expansion into pristine habitat selecting for high fecundity genotypes at the expense of schistosome resistance, presenting S. mansoni with genetically homogenous highly fecund susceptible populations around the focal point, promoting development of a highly compatible host-parasite relationship. Longitudinal study of such systems may prove important in predicting public health risks engendered by future environmental engineering projects.

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Genetic diversity, fixation and differentiation of the freshwater snail Biomphalaria pfeifferi (Gastropoda, Planorbidae) in arid lands

et al. 2013

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The freshwater snail Biomphalaria pfeifferi is the main intermediate host of human intestinal Bilharziasis. It is widely distributed in Africa, Madagascar and middle-eastern countries, and its habitat includes wetlands, and arid to semi-arid areas. Based on analysis of 18 microsatellites, we investigated reference allelic variation among 30 populations of B. pfeifferi from three drainage basins in Dhofar, Oman (the eastern limit of its distribution). This is an arid to semi-arid region, with a 9,000-year history of very low rainfall, but is subject to unpredictable and destructive flash floods. In this context we showed that genetic fixation was very high compared to genetic differentiation which was moderate and, that, relative to B. pfeifferi populations from wetlands, the populations in Dhofar show evidence of lower levels of genetic diversity, a higher degree of genetic fixation, a quasi-absence of migration, and a higher level of genetic drift. Despite the extreme conditions in the Dhofar habitat of this species, it is able to survive because of its very high self-fertilization (approaching 100 %) and fecundity rates.

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Microsatellite variation in the freshwater snail Biomphalaria pfeifferi

Cited by 18 publications

References 4 publications

A Spatial and Temporal Approach to Microevolutionary Forces Affecting Population Biology in the Freshwater SnailBiomphalaria pfeifferi

A Spatial and Temporal Approach to Microevolutionary Forces Affecting Population Biology in the Freshwater SnailBiomphalaria pfeifferi

Low genetic diversity in a snail intermediate host (Biomphalaria pfeifferi Krass, 1848) and schistosomiasis transmission in the Senegal River Basin

Genetic diversity, fixation and differentiation of the freshwater snail Biomphalaria pfeifferi (Gastropoda, Planorbidae) in arid lands

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