Microsatellite variability reveals beekeeping influences on Iberian honeybee populations

Cánovas, Fernando; Rúa, Pilar De la; Serrano, José; Galián, José

doi:10.1007/s13592-011-0020-1

Cited by 41 publications

(30 citation statements)

References 41 publications

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“…We noticed that over half of all HNHK individuals were of the CH (13/30) and hybrid (5/30) types (Supplementary file 4; Figure 1b). Similar introgression has been reported in A. mellifera populations Cánovas et al 2011). The unusual Hainan race is particularly sensitive to such introgression owing to its narrow distribution and limited population size.…”

Section: Discussionsupporting

confidence: 80%

Phylogeography of Apis cerana populations on Hainan island and southern mainland China revealed by microsatellite polymorphism and mitochondrial DNA

et al. 2016

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-In this study, we reexamined the patterns of divergence and phylogeographic structure among Apis cerana populations on Hainan island and southern mainland China. Analysis of 10 microsatellite loci from 12 geographically distinct A. cerana populations identified a total of 151 alleles, with a range of 7 to 24 at each locus. The level of genetic variation (observed heterozygosity) within each population ranged from 0.603 to 0.661, while variation within populations contributed most (93.78-98.84 %) of the molecular variance. Microsatellite data revealed three differentiated groups, one including all the mainland populations while the other two containing one of and the other three island populations, respectively. Severe genetic introgressions from mainland China into Hainan island were apparent, and these were characterized at both population and individual levels using combined analysis of microsatellites and mitochondrial DNA.Apis cerana / microsatellite / phylogeographic structure / genetic introgression

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Section: Discussionsupporting

confidence: 80%

Phylogeography of Apis cerana populations on Hainan island and southern mainland China revealed by microsatellite polymorphism and mitochondrial DNA

et al. 2016

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“…For example, Kandemir et al (2006) detected two distinct mitochondrial lineages C and O, although the overall Cyprus population was relatively homogenous in terms of microsatellites. Similar disagreement between mitochondrial and nuclear markers occurs in Africanized honey bees (Lobo 1995) and in A. m. iberica (Cánovas 2008(Cánovas , 2011. In studies of subspecies identification, it was recommended to use mtDNA only for initial screening (Rortais et al 2011) or together with morphometrics or nuclear markers (Nielsen et al 1999;Pinto et al 2003).…”

Section: Discussionmentioning

confidence: 97%

Wing geometric morphometrics and microsatellite analysis provide similar discrimination of honey bee subspecies

Oleksa¹,

Tofilski²

2014

Apidologie

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-Identification of honey bee (Apis mellifera) subspecies is important for their protection. It is also used by queen breeders to maintain some breeding lines. In this study, we compared three methods of subspecies identification based on the following: 17 microsatellite loci, COI-COII mitotypes and geometric morphometrics of forewing venation. The methods were used to classify colonies and workers from a mixed population of A. m. mellifera and A. m. carnica. There was highly significant correlation between results obtained using the three methods. More than three quarters of colonies were classified to the same subspecies by all three methods. The agreement was highest between microsatellites and morphometrics. More than 90 % of colonies were classified to the same subspecies by the two methods. There was also relatively high agreement (75 %) between microsatellites and morphometrics when workers were classified as pure subspecies or hybrids. In particular, one pure subspecies was never misclassified as other pure subspecies. The results presented here show that morphometrics can be used for detection of hybrids between A. m. mellifera and A. m. carnica. geometric morphometrics / subspecies discrimination / microsatellites / mtDNA / Bayesian clustering / Apis mellifera / phenotype-genotype correlation

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“…According to Cánovas et al. (, ), the North African honey bees (A lineage) have colonized southwest of Europe (M lineage) and there was hybridization between lineages. Our results confirm this finding because on the neighbor‐joining phylogenetic tree, the H4, H13, H14 from Spain and H20 (Melli4) were closely related with the M lineage, and the H17 and H19 (Iberi2) also from Spain had closer connection with the A lineage (Fig.…”

Section: Discussionmentioning

confidence: 99%

Population structure of honey bees in the Carpathian Basin (Hungary) confirms introgression from surrounding subspecies

Oleksa

Borowik

Kusza

2015

Ecology and Evolution

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Carniolan honey bees (Apis mellifera carnica) are considered as an indigenous subspecies in Hungary adapted to most of the ecological and climatic conditions in this area. However, during the last decades Hungarian beekeepers have recognized morphological signs of the Italian honey bee (Apis mellifera ligustica). As the natural distribution of the honey bee subspecies can be affected by the importation of honey bee queens or by natural gene flow, we aimed at determining the genetic structure and characteristics of the local honey bee population using molecular markers. All together, 48 Hungarian and 84 foreign (Italian, Polish, Spanish, Liberian) pupae and/or workers were used for mitochondrial DNA analysis. Additionally, 53 sequences corresponding to 10 subspecies and the Buckfast hybrid were downloaded from GenBank. For the nuclear analysis, 236 Hungarian and 106 foreign honey bees were genotyped using nine microsatellites. Heterozygosity values, population‐specific alleles, FST values, principal coordinate analysis, assignment tests, structure analysis, and dendrograms were calculated. Haplotype and nucleotide diversity values showed moderate values. We found that one haplotype (H9) was dominant in Hungary. The presence of the black honey bee (Apis mellifera mellifera) was negligible, but a few individuals resembling other subspecies were identified. We proved that the Hungarian honey bee population is nearly homogeneous but also demonstrated introgression from the foreign subspecies. Both mitochondrial DNA and microsatellite analyses corroborated the observations of the beekeepers. Molecular analyses suggested that Carniolan honey bee in Hungary is slightly affected by Italian and black honey bee introgression. Genetic differences were detected between Polish and Hungarian Carniolan honey bee populations, suggesting the existence of at least two different gene pools within A. m. carnica.

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Microsatellite variability reveals beekeeping influences on Iberian honeybee populations

Cited by 41 publications

References 41 publications

Phylogeography of Apis cerana populations on Hainan island and southern mainland China revealed by microsatellite polymorphism and mitochondrial DNA

Phylogeography of Apis cerana populations on Hainan island and southern mainland China revealed by microsatellite polymorphism and mitochondrial DNA

Wing geometric morphometrics and microsatellite analysis provide similar discrimination of honey bee subspecies

Population structure of honey bees in the Carpathian Basin (Hungary) confirms introgression from surrounding subspecies

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