Microsatellite tagging of the stripe-rust resistance gene YrH52 derived from wild emmer wheat, Triticum dicoccoides, and suggestive negative crossover interference on chromosome 1B

Microsatellite high‐density mapping of the stripe rust resistance gene YrH52 region on chromosome 1B and evaluation of its marker‐assisted selection in the F2 generation in wild emmer wheat

“…Young leaf samples were collected from each F # individual in the glasshouse, frozen in liquid nitrogen, and stored at k80mC. Genomic DNA was extracted by use of plant genomic DNA isolation reagent DNAzol4ES with some modifications (Peng et al, 1999).…”

“…Stripe rust challenge tests on 150 F $ families of the mapping population were conducted during the 1997-98 cropping season at the Bet Dagan Experimental Farm of the Volcani Center in central Israel, and at Neve Yaar Agricultural Research Center in northern Israel (Peng et al, 1999). Based on the reaction of F $ families to stripe rust, the genotypes of the corresponding F # individual plants were determined : homozygous resistant (RR, all plants in the F $ family were resistant) ; heterozygous resistant (Rr, most of the plants in the F $ family were resistant and the minority were susceptible) ; and homozygous susceptible (rr, all plants in the F $ family were susceptible).…”

“…Van Silfhout et al (1989) tested about 850 wild emmer accessions collected from 31 locations using 28 stripe rust isolates from 19 countries, and found that 19 (28%) of the 68 promising resistant accessions derived from Mount Hermon. A new stripe rust resistance gene, tentatively named YrH52, carried by accession Hermon H52 from the unique Mount Hermon population of Israeli wild emmer wheat, was recently mapped onto chromosome 1B (Peng et al, 1999).…”

“…The T. dicoccoides-derived stripe rust resistance gene YrH52 was accordingly located with the aid of this type of markers. But there is a gap of 24.5 cM on the distal side that may negatively affect the markerassisted selection and map-based cloning of this gene (Peng et al, 1999). In the case of selection for quantitative traits, marker-assisted selection programmes can be undertaken (Lande & Thompson, 1990), but it is hard to evaluate their potential efficiency in the absence of sufficient data on the relation between genotype and phenotype (Hospital et al, 1992).…”

AB-QTL analysis in winter wheat: I. Synthetic hexaploid wheat (T. turgidum ssp. dicoccoides × T. tauschii) as a source of favourable alleles for milling and baking quality traits

“…dicoccoides) should also be useful, allowing detection of favourable alleles from both the tetraploid and diploid exotic parents. Wild emmer proved already as a donor of useful genetic variation for important agronomic traits in tetra-and hexaploid wheat, contributing to powdery mildew resistance, rust resistance, reduced plant height, early heading, increased number of spikes per plant, higher spike weight per plant, increased kernels per spikelet and higher yield (Peng et al 1999(Peng et al , 2003Rong et al 2000). Wild emmer also proved to possess genes improving grain quality in wheat.…”