Microsatellite primers forCamissoniopsis cheiranthifolia(Onagraceae) and cross‐amplification in related species

Abstract

• Premise of the study: We developed 24 nuclear microsatellite primers from an enriched genomic library for the Pacific coastal dune endemic Camissoniopsis cheiranthifolia to study the consequences of mating system differentiation, the genetics of species’ range limits, and hybridization with its closest sister taxon, C. bistorta.• Methods and Results: Twenty-four primer pairs were developed and characterized in four populations of C. cheiranthifolia and one population of C. bistorta. We also tested eight additional taxa for cross-amplification. The average number of alleles per locus per species was 4.3 and 6.0, respectively. The number of loci that amplified and were variable within the eight related taxa ranged from six to 17.• Conclusions: These markers will be useful in studying mating system evolution, the genetic structure of species’ ranges, hybridization, and the provenance of material used for habitat restoration in C. cheiranthifolia, C. bistorta, and related species.

Consequences of multiple mating‐system shifts for population and range‐wide genetic structure in a coastal dune plant

“…Following guidelines that a sample of 20–30 individuals assayed for 10–20 polymorphic nuclear microsatellites (nSSR) yields reliable estimates of most population genetic parameters (Hale, Burg, & Steeves, ), we genotyped ~25 individuals/population (mean/population = 25.97, total n = 987, Table ) for 13 polymorphic nSSR loci (B11, B34, B59, C19, C49, C67, C89, C110, C133, C135b, E30, E70, E42) following López‐Villalobos, Samis, and Eckert (). We assayed 10% of samples twice to ensure repeatability.…”

Strong genetic differentiation but not local adaptation toward the range limit of a coastal dune plant

“…At all sites, we sampled ࣙ500 m from transplant plots to avoid transplant experiment descendants. Each sample was genotyped for 13 polymorphic microsatellite loci following López-Villalobos et al (2014). We quantified within-population genetic diversity using several complementary measures: total number of alleles detected (A), total number of private alleles (A p ), rarefied number of alleles per locus (A r ) using 1000 bootstrap samples of the smallest sample size (n = 20), observed (H o ) and expected heterozygosities (H e ), and the inbreeding coefficient (F) estimated using the R package DiveRsity (v. 1.9.73, Keenan et al 2013).…”

The contribution of hybridization to range‐wide population genetic structure in a Pacific coastal dune plant

“…Moreover, almost all of the populations sampled for this study were located from data on herbarium specimens that had been identified to species. López-Villalobos and Eckert (2018) assayed 805 individuals from 32 mainland populations across the range of C. cheiranthifolia (mean = 25 per population) for variation at 13 nuclear microsatellite markers (nSSR; see methods of López-Villalobos et al, 2014) and a subset of these individuals (124 from 27 populations) for variation at nine polymorphic chloroplast DNA microsatellites (cpSSR; following Weising and Gardner, 1999;Chung and Staub, 2003). We used those data here.…”