Microsatellite polymorphism for molecular characterization of pomelo (Citrus maxima) accessions from Indonesia

Abstract

Abstract. Susandarini R, Subandiyah S, Daryono BS, Rugayah. 2020. Microsatellite polymorphism for molecular characterization of pomelo (Citrus maxima) accessions from Indonesia. Biodiversitas 21: 2390-2395. Citrus maxima (Burm.) Merr. (pomelo) as a minor fruit crop deserve attention on its phenotypic and genotypic variability to avoid the risk of extinction. Previous study showed that pomelo from Indonesia has high morphological variability, and thus it is interesting to explore its genotypic variability using molecular markers. Microsatellite is a molecular marker widely used in Citrus taxonomy studies. This study aimed at revealing microsatellite polymorphism and its potential application in cultivar characterization of C .maxima. Eighty accessions of C. maxima consisting of registered cultivars and landraces from Indonesia were used in this study. Analysis of microsatellite sequences from genomic DNA amplified using DY296883 primer showed that C. maxima microsatellite has high polymorphism in the form of repeat length variation of (GA)n, ranging from (GA)7 to (GA)19. This study proved the existence of high genotypic variability in C. maxima, and confirmed the role of microsatellite as a useful molecular marker for uncovering variability at intraspecific level. Observation of the microsatellite polymorphisms indicated that variability of (GA)n can be used to distinguish some pomelo cultivars.

Chemical Content and Pharmacology of Pomelo Orange (Citrus Maxima) Fruit Peel: A Review

“…Pomelo (Citrus maxima, previously known as Citrus grandis) is a perennial fruit plant that belongs to the Rutaceae family [6]. The naming of this fruit varies in each region, as listed in The pomelo plant is a species of tropical fruit plant originating from Southeast Asia, which yields fruit twice a year.…”

“…The pomelo plant thrives in tropical lowlands up to an altitude of 400 meters, with an average temperature of 25-32°C and rainfall ranging from 1,500-2,500 mm, accompanied by a dry period of 3-4 months. Its fruit is large and round, with a diameter of 10-30 cm, consisting of thick skin (flavedo as the outer skin and albedo as the inner skin), which makes up 30% of its weight, and its flesh is pink or white with a sweet, bland, or sweet-tart taste [3,[6][7][8][9]. The picture of pomelo is shown in Figure 1.…”

Short Communication: Analysis of simple sequence repeats variations for the assessment of genotypic diversity in Centella asiatica (L.) Urb.

“…The SSR sequences amplified using mCaCIR002 primers in this study showed high variability in the number of (CT)n repeats representing microsatellite polymorphism become strong evidence on the intraspecific variability in C. asiatica. Previous studies showed that microsatellites polymorphism caused by mutations can be used as genetic markers for evolutionary studies, phylogenetic analysis, gene flow, paternity analysis, and population structure analysis (Milner et al 2013;Mehrotra and Goyal 2014;Putman and Carbone 2014;Vieira et al 2016;Susandarini et al 2020).…”

Analysis of stem rot disease severity on Pomelo (Citrus maxima) plantation in Pangkep Regency, South Sulawesi

“…Pomelo (Citrus maxima) are potential plants to be developed in Indonesia, the production center of Pomelo South Sulawesi is Pangkep Regency [1] which contributes more than 80% of production [2]. South Sulawesi's contribution nationally to the supply of Pomelo ranks first, with 30.76%.…”