Microsatellite markers reveal clear geographic structuring among threatened noble crayfish (Astacus astacus) populations in Northern and Central Europe

Gross, Riho; Palm, Stefan; Kõiv, Kuldar; Prestegaard, Tore; Jussila, Japo; Paaver, Tiit; Geist, Juergen; Kokko, Hanna; Karjalainen, Anna K.; Edsman, Lennart

doi:10.1007/s10592-013-0476-9

Cited by 28 publications

(50 citation statements)

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“…The present analysis indicates that Greece is an older glacial refugium than the eastern Black Sea (phylogenetic clade G4) and Western Balkans (phylogenetic clade G3), with the latter two regions already identified by Schrimpf et al [29]. Nevertheless, the scenario of multiple refugia is confirmed, as suggested for the noble crayfish [17, 29] and postulated for other species (e.g. [90, 91]).…”

Section: Discussionsupporting

confidence: 81%

Microevolution of the noble crayfish (Astacus astacus) in the Southern Balkan Peninsula

et al. 2017

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BackgroundThe noble crayfish (Astacus astacus) displays a complex historical and contemporary genetic status in Europe. The species divergence has been shaped by geological events (i.e. Pleistocene glaciations) and humanly induced impacts (i.e. translocations, pollution, etc.) on its populations due to species commercial value and its niche degradation. Until now, limited genetic information has been procured for the Balkan area and especially for the southernmost distribution of this species (i.e. Greece). It is well known that the rich habitat diversity of the Balkan Peninsula offers suitable conditions for genetically diversified populations. Thus, the present manuscript revisits the phylogenetic relationships of the noble crayfish in Europe and identifies the genetic make-up and the biogeographical patterns of the species in its southern range limit.ResultsMitochondrial markers (i.e. COI and 16S) were used in order to elucidate the genetic structure and diversity of the noble crayfish in Europe. Two of the six European haplotypic lineages, were found exclusively in Greece. These two lineages exhibited greater haplotypic richness when compared with the rest four (of “Central European” origin) while they showed high genetic diversity. Divergence time analysis identified that the majority of this divergence was captured through Pleistocene, suggesting a southern glacial refugium (Greece, southern Balkans). Furthermore, six microsatellite markers were used in order to define the factors affecting the genetic structure and demographic history of the species in Greece. The population structure analysis revealed six to nine genetic clusters and eight putative genetic barriers. Evidence of bottleneck effects in the last ~5000 years (due to climatic and geological events and human activities) is also afforded. Findings from several other research fields (e.g. life sciences, geology or even archaeology) have been utilized to perceive the genetic make-up of the noble crayfish.ConclusionsThe southernmost part of Balkans has played a major role as a glacial refugium for A. astacus. Such refugia have served as centres of expansion to northern regions. Recent history of the noble crayfish in southern Balkans reveals the influence of environmental (climate, geology and/or topology) and anthropogenic factors.Electronic supplementary materialThe online version of this article (doi:10.1186/s12862-017-0971-6) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 81%

Microevolution of the noble crayfish (Astacus astacus) in the Southern Balkan Peninsula

et al. 2017

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“…Although the calibrated molecular clock is yet unavailable for the noble crayfish, the rate for the mitochondrial evolution in general seems to be very similar in different species with different generation times, being roughly 1−2%/site/My (Stoeckle et al, 2014). Therefore, nuclear markers, like microsatellites could provide more information of possible adaptive lineages and diversification of the noble crayfish populations in Finland (Gross et al, 2013). The microsatellite markers exploited in small scale for Finnish noble crayfish populations showed, that variation in the nuclear DNA level exists also in Northern populations of A. astacus (Gross et al, 2013).…”

Section: Figure 2 Maximum Likelihood (Ml) -Tree Of the Sequenced Coi-mentioning

confidence: 99%

“…Therefore, nuclear markers, like microsatellites could provide more information of possible adaptive lineages and diversification of the noble crayfish populations in Finland (Gross et al, 2013). The microsatellite markers exploited in small scale for Finnish noble crayfish populations showed, that variation in the nuclear DNA level exists also in Northern populations of A. astacus (Gross et al, 2013).…”

Section: Figure 2 Maximum Likelihood (Ml) -Tree Of the Sequenced Coi-mentioning

confidence: 99%

“…Previous studies concerning the noble crayfish diversity in Finland (Alaranta et al, 2006(Alaranta et al, , 2011 have been based on the microsatellite-like repeat located in the internal transcribed spacer 1-region (Edsman et al, 2002), or on the microsatellites on a limited number of populations (Gross et al, 2013). On the European wide scale, also mitochondrial cytochrome oxidase I (COI) gene and 16S rDNA-gene have been utilized to explore the biogeography and diversity of the species (Schrimpf et al, 2011(Schrimpf et al, , 2014.…”

Section: Introductionmentioning

confidence: 99%

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Mitochondrial cytochrome oxidase I gene analysis indicates a restricted genetic background in Finnish noble crayfish(Astacus astacus)stocks

Makkonen

Kokko

Jussila

2015

Knowl. Manag. Aquat. Ecosyst.

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Key-words:noble crayfish, native species, population decline, genetic diversity, cytochrome oxidase IThe IUCN Red List indexes the noble crayfish (Astacus astacus) as vulnerable, with a declining population trend. The main threats to the species are the crayfish plague caused by the oomycete Aphanomyces astaci and the introduced North American crayfish that act as the carriers of this disease. In Finland, the noble crayfish is considered as a native species, which original distribution area covers the southern part of the country, but the species distribution has been dispersed to cover almost the whole country. The aim of this study was to survey the genetic diversity among the Finnish noble crayfish populations. The mitochondrial cytochrome oxidase I (COI)-gene was sequenced from 742 individuals representing 59 populations from Finland and Estonia. As a result, only a single haplotype was found. Based on these results, the genetic diversity of noble crayfish in its Northern distribution range is remarkably low. The observed lack of variation can result from several mechanisms including small size of the founder population and the intense spreading of the species by manmade stockings. The restricted diversity can also be caused by eradication of the original populations due to crayfish plague epidemics and spreading of the invasive crayfish species carrying the crayfish plague. It is also possible that all contemporary Finnish noble crayfish populations originate from stockings with no variation in respect to COI-gene. RÉSUMÉ L'analyse du gène mitochondrial du cytochrome oxydase I indique un fond génétique restreint dans les populations finlandaises de l'écrevisse à pattes rouges (Astacus astacus)

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“…It was thought this high difference to be related from geographic distance. Gross et al (2013) developed 10 new microsatellite markers for reveal geographic structiring of A. astacus. It is a first large scale study for this species.…”

Section: Microsatellitesmentioning

confidence: 99%

Kerevitlerde Genetik Şifre Belirlemenin Önemi ve Kullanılan Yöntemler

Bektaş¹

2018

Journal of Limnology and Freshwater Fisheries Research

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Crayfish are one of many freshwater organisms that are subject to biodiversity changes. The crayfish stocks in the world and Turkey have been damaged due to the crayfish plaque caused by Aphanomyces astaci, overfishing water pollution and other causes. For the continuation of endangered stocks of these species, it is necessary to protect the populations and restocking into apropriate environment. Environmental factors have an vital role in the genetic structure of a population. So the genetic diversity between population is vital because the information of the genetic construction of population is curical due to the ensuring sustainability and conservation of these species when evaluated with other factors but, unfortunately, still restricted. With this review, the genetic study conducted so far will be discussed to try getting important information for preservation and management of crayfish, because preservation of genetic diversity is very important for survival or persistence of these species such a long time.Keywords: Genetic diversity, fisheries, protection, biodiversity, molecular identification REVIEW Received Kerevitlerde Genetik Şifre Belirlemenin Önemi ve Kullanılan YöntemlerÖz: Kerevitler biyoçeşitlilik değişimlerine maruz kalan birçok tatlı su organizmalarından biridir. Dünyada ve Türkiye'de kerevit stokları Aphanomyces astaci fungusunun neden olduğu hastalık, aşırı avcılık, su kirliliği ve diğer nedenlerden tükenme tehlikesiyle karşı karşıya kalmıştır. Stokları tehlikede olan bu canlıların devamı için populasyonların korunması ve uygun çevrelere yeniden stoklanması gerekmektedir. Çevresel faktörler bir populasyonun genetik kompozisyonunda çok önemli rol oynar. Bu yüzden populasyonlar arasındaki genetik çeşitlilik büyük öneme sahiptir çünkü populasyonların genetik yapılarının bilinmesi bu türün devamının sağlanması ve korunması açısından hayatidir fakat ne yazıkki bu konuda yapılan çalışmalar hala sınırlıdır. Bu derlemeyle şimdiye kadar yapılan genetik çalışmalar tartışılarak kerevitlerin korunması ve yönetimi için önemli bilgiler elde edilmeye çalışılacaktır çünkü genetik çeşitliliğin korunması bu türlerin uzun süre yaşaması ya da varlığını sürdürmesi için çok önemlidir.

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Microsatellite markers reveal clear geographic structuring among threatened noble crayfish (Astacus astacus) populations in Northern and Central Europe

Cited by 28 publications

References 47 publications

Microevolution of the noble crayfish (Astacus astacus) in the Southern Balkan Peninsula

Microevolution of the noble crayfish (Astacus astacus) in the Southern Balkan Peninsula

Mitochondrial cytochrome oxidase I gene analysis indicates a restricted genetic background in Finnish noble crayfish(Astacus astacus)stocks

Kerevitlerde Genetik Şifre Belirlemenin Önemi ve Kullanılan Yöntemler

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