Microsatellite markers isolated from Cabomba aquatica s.l. (Cabombaceae) from an enriched genomic library

Abstract

Premise of the study:Microsatellite primers were designed for the submersed aquatic plant Cabomba aquatica s.l. (Cabombaceae) and characterized to estimate genetic diversity parameters.Methods and Results:Using a selective hybridization method, we designed and tested 30 simple sequence repeat loci using two natural populations of C. aquatica s.l., resulting in 13 amplifiable loci. Twelve loci were polymorphic, and alleles per locus ranged from two to four across the 49 C. aquatica s.l. individuals. Observed heterozygosity, expected heterozygosity, and fixation index varied from 0.0 to 1.0, 0.0 to 0.5, and −1.0 to −0.0667, respectively, for the Manaus population and from 0.0 to 1.0, 0.0 to 0.6, and −1.0 to 0.4643 for the Viruá population.Conclusions:The developed markers will be used in further taxonomic and population studies within Cabomba. This set of microsatellite primers represents the first report on rapid molecular markers in the genus.

Aquatic Plant Genomics: Advances, Applications, and Prospects

“…is a small genus in the family Cabombaceae, containing 5 species whose identification is problematic due to vegetative similarity among the taxa. Thus in 2015, 13 SSR loci were developed to investigate the genetic structure of C. aquatica and assess the validity of the five recognized species [ 18 ]. Another example is the sacred lotus ( Nelumbo nucifera ), economically and ornamentally important in China.…”