Microsatellite Markers in Hazelnut: Isolation, Characterization, and Cross-species Amplification

Abstract

<jats:p>Three microsatellite-enriched libraries of the european hazelnut (<jats:italic>Corylus avellana</jats:italic> L.) were constructed: library A for CA repeats, library B for GA repeats, and library C for GAA repeats. Twenty-five primer pairs amplified easy-to-score single loci and were used to investigate polymorphism among 20 <jats:italic>C. avellana</jats:italic> genotypes and to evaluate cross-species amplification in seven <jats:italic>Corylus</jats:italic> L. species. Microsatellite alleles were estimated by fluorescent capillary electrophoresis fragment sizing. The number of alleles per locus ranged from 2 to 12 (average = 7.16) in <jats:italic>C. avellana</jats:italic> and from 5 to 22 overall (average = 13.32). With the exception of CAC-B110, di-nucleotide SSRs were characterized by a relatively large number of alleles per locus (≥5), high average observed and expected heterozygosity (H<jats:sub>o</jats:sub> and H<jats:sub>e</jats:sub> &gt; 0.6), and a high mean polymorphic information content (PIC ≥ 0.6) in <jats:italic>C. avellana</jats:italic>. In contrast, tri-nucleotide microsatellites were more homozygous (H<jats:sub>o</jats:sub> = 0.4 on average) and less informative than di-nucleotide simple sequence repeats (SSRs) as indicated by a lower mean number of alleles per locus (4.5), H<jats:sub>e</jats:sub> (0.59), and PIC (0.54). Cross-species amplification in <jats:italic>Corylus</jats:italic> was demonstrated. These microsatellite markers were highly heterozygous and polymorphic and differentiated among genotypes of <jats:italic>C. avellana</jats:italic> irrespective of geographical origin. They will aid in fingerprinting genotypes of the european hazelnut and other <jats:italic>Corylus</jats:italic> species, genome mapping, and genetic diversity assessments.</jats:p>

Transferability of rice SSR markers to bamboo

“…Intrageneric cross-amplification of fescue SSR markers in grass gave 3.98 alleles per SSR locus (Saha et al 2006). These results suggest that our use of more distantly related bamboo species may have resulted in a higher number of alleles per SSR locus (Bassil et al 2005) and that the flanking region of the rice SSR markers may have been conserved and duplicated in these bamboo species (Chen et al 2002). Transferability, the ratio of amplified markers/tested markers Effect of SSR markers with different motif types Peakall et al (1998) reported that SSR markers can be classified into three different motif types, namely, pure, compound, and interrupted.…”

Genetic diversity revealed by morphological traits and ISSR markers in hazelnut germplasm from northern Spain

“…Compared with other species, the number of microsatellites described for hazelnut is limited (18 microsatellites were described by Boccacci et al. 2005 and 33 microsatellites by Bassil et al. 2005a,b), indicating a need for continued efforts to develop new molecular markers.…”

“…2005). In addition, a set of 51 SSR markers has been developed (Bassil et al. 2005a,b, Boccacci et al.…”

Characterization of European hazelnut (Corylus avellana) cultivars using SSR markers

“…(Cabe et al 2005;Hokanson et al 2001), apricot (Prunus armeniaca L.) (Romero et al 2003), peach [Prunus persica (L.) Batsch] (Aranzana et al 2002;Testolin et al 2000) grape (Vitis vinifera L.) (Dangl et al 2001;Fatahi et al 2003;Martin et al 2003), and pistachio (Pistacia vera L.) (Ahmad et al 2003). In hazelnut, simple sequence repeat (SSR) markers have been developed by Bassil et al (2005) and Boccacci et al (2005).…”