Microsatellite markers for the roman, <i>Chrysoblephus laticeps</i> (Teleostei: Sparidae), an overexploited seabream from South Africa

Teske, Peter R.; Cowley, Paul D.; Forget, Fabien R. G.; Beheregaray, Luciano B.

doi:10.1111/j.1755-0998.2009.02595.x

Cited by 8 publications

(7 citation statements)

References 7 publications

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“…Previous studies in patients with heart failure [18], chronic PH [19], arrhythmogenic right ventricular disease [20] and in patients referred for cardiac surgery [21] have found that strain imaging is more precise in the quantification of RV function than the global parameters RVFAC and TAPSE. The complex shape of the right ventricle and the monoplane measurement are one reason why RVFAC has to be used with caution.…”

Section: Discussionmentioning

confidence: 99%

Right Ventricular Function Quantification in Takotsubo Cardiomyopathy Using Two-Dimensional Strain Echocardiography

et al. 2014

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AimsThis study sought to characterize global and regional right ventricular (RV) myocardial function in patients with Takotsubo cardiomyopathy (TC) using 2D strain imaging.MethodsWe compared various parameters of RV and left ventricular (LV) systolic function between 2 groups of consecutive patients with TC at initial presentation and upon follow-up. Group 1 had RV involvement and group 2 did not have RV involvement.ResultsAt initial presentation, RV peak systolic longitudinal strain (RVPSS) and RV fractional area change (RVFAC) were significantly lower in group 1 (−13.2±8.6% vs. −21.8±5.4%, p = 0.001; 30.7±9.3% vs. 43.5±6.3%, p = 0.001) and improved significantly upon follow-up. Tricuspid annular plane systolic excursion (TAPSE) did not differ significantly at initial presentation between both groups (14.8±4.1 mm vs. 17.9±3.5 mm, p = 0.050). Differences in regional systolic RV strain were only observed in the mid and apical segments. LV ejection fraction (LVEF) and LV global strain were significantly lower in group 1 (36±8% vs. 46±10%, p = 0.006 and −5.5±4.8% vs. −10.2±6.2%, p = 0.040) at initial presentation. None of the parameters were significantly different between the 2 groups upon follow-up. A RVPSS cut-off value of >−19.1% had a sensitivity of 85% and a specificity of 71% to discriminate between the 2 groups.ConclusionIn TC, RVFAC, RVPSS, LVEF and LV global strain differed significantly between patients with and without RV dysfunction, whereas TAPSE did not. 2 D strain imaging was feasible for the assessment of RV dysfunction in TC and could discriminate between patients with and without RV involvement in a clinically meaningful way.

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Section: Discussionmentioning

confidence: 99%

Right Ventricular Function Quantification in Takotsubo Cardiomyopathy Using Two-Dimensional Strain Echocardiography

et al. 2014

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“…2009) of which 41 (36%) are commonly found around the southern African coast (Heemstra & Heemstra 2004). Although sparids are a prominent feature to the South African fishery, microsatellite markers have only been developed for one species, the roman Chrysoblephus laticeps (Teske et al. 2009).…”

Section: Introductionmentioning

confidence: 99%

“…The polymorphic markers isolated were tested with four previously isolated microsatellites from other studies, (Piñera et al. 2006; Teske et al. 2009), across nine additional species.…”

Section: Introductionmentioning

confidence: 99%

High‐throughput microsatellite marker development in two sparid species and verification of their transferability in the family Sparidae

Reid

Hoareau

Bloomer

2012

Molecular Ecology Resources

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Recently, 454 sequencing has emerged as a popular method for isolating microsatellites owing to cost-effectiveness and time saving. In this study, repeat-enriched libraries from two southern African endemic sparids (Pachymetopon blochii and Lithognathus lithognathus) were 454 GS-FLX sequenced. From these, 7370 sequences containing repeats (SCRs) were identified. A brief survey of 23 studies showed a significant difference between the number of SCRs when enrichment was performed first before 454 sequencing. We designed primers for 302 unique fragments containing more than five repeat units and suitable flanking regions. A fraction (<11%) of these loci were characterized with 18 polymorphic microsatellite loci (nine in each of the focal species) being described. Sanger sequencing of alleles confirmed that size variation was because of differences in the number of tandem repeats. However, a case of homoplasy and sequencing errors in the 454 sequencing were identified. These newly developed and four previously isolated loci were successfully used to identify polymorphic markers in nine other economically important species, representative of sparid diversity. The combination of newly developed markers with data from previous sparid cross-species studies showed a significant negative correlation between genetic divergence to focal species and microsatellite transferability. The high level of transferability we described (48% amplification success and 32% polymorphism) suggests that the 302 microsatellite loci identified represent an excellent resource for future studies on sparids. Microsatellite marker development should commonly include tests of transferability to reduce costs and increase feasibility of population genetics studies in nonmodel organisms.

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“…Fifteen microsatellite markers were used in this study; LLt002,LLt005,LLt006,LLt007,LLt011,LLt014,LLt020,LLt024 and LLtr004 were isolated in white steenbras (from Reid et al 2012), while PBt003, PBt007, PBt013, PBt018, PB106 (from Reid et al 2012 and Clat11 (from Teske et al 2009) were isolated from related species. These loci were designed into multiplexes and amplified using the Quantitect ® Multiplex PCR Kit (QIAGEN ® ).…”

Section: Mitochondrial Control Region Sequencing and Microsatellite Gmentioning

confidence: 99%

Genetic stock structure of white steenbrasLithognathus lithognathus(Cuvier, 1829), an overexploited fishery species in South African waters

Bennett

Reid

Gouws

et al. 2017

African Journal of Marine Science

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White steenbras (Lithognathus lithognathus, Sparidae) is an overexploited fishery species endemic to South Africa. Overexploitation in recreational, subsistence and commercial fisheries has resulted in stock collapse, and the need for improved management of the species. Adults are thought to undertake large scale annual spawning migrations, yet movement studies indicate low levels of connectivity among coastal regions. To address this, mitochondrial DNA sequencing and genotyping of microsatellite loci in the nuclear genome were conducted to determine the genetic stock structure and level of gene flow within this species. Genetic diversity was high throughout the species' core distribution, with no evidence of isolation by distance or localised spawning. Low, non-significant pairwise fixation indices (FST, RST and Jost's Dest) indicated low genetic differentiation and high levels of gene flow. The observed results, and agreement between mitochondrial and microsatellite DNA, confirm that white steenbras exists as a single genetic stock with high levels of gene flow throughout its distribution.

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Microsatellite markers for the roman, Chrysoblephus laticeps (Teleostei: Sparidae), an overexploited seabream from South Africa

Cited by 8 publications

References 7 publications

Right Ventricular Function Quantification in Takotsubo Cardiomyopathy Using Two-Dimensional Strain Echocardiography

Right Ventricular Function Quantification in Takotsubo Cardiomyopathy Using Two-Dimensional Strain Echocardiography

High‐throughput microsatellite marker development in two sparid species and verification of their transferability in the family Sparidae

Genetic stock structure of white steenbrasLithognathus lithognathus(Cuvier, 1829), an overexploited fishery species in South African waters

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