MICROSATELLITE MARKERS FOR THE MOLECULAR CHARACTERISATION OF POTENTIALLY COMMERCIAL MANGO (Mangifera indica) PROGENIES

The breeding of tropical fruit trees for improving fruit traits is complicated, due to the long juvenile phase, generation cycle, parthenocarpy, polyploidy, polyembryony, heterozygosity and biotic and abiotic factors, as well as a lack of good genomic resources. Many molecular techniques have recently evolved to assist and hasten conventional breeding efforts. Molecular markers linked to fruit development and fruit quality traits such as fruit shape, size, texture, aroma, peel and pulp colour were identified in tropical fruit crops, facilitating Marker-assisted breeding (MAB). An increase in the availability of genome sequences of tropical fruits further aided in the discovery of SNP variants/Indels, QTLs and genes that can ascertain the genetic determinants of fruit characters. Through multi-omics approaches such as genomics, transcriptomics, metabolomics and proteomics, the identification and quantification of transcripts, including non-coding RNAs, involved in sugar metabolism, fruit development and ripening, shelf life, and the biotic and abiotic stress that impacts fruit quality were made possible. Utilizing genomic assisted breeding methods such as genome wide association (GWAS), genomic selection (GS) and genetic modifications using CRISPR/Cas9 and transgenics has paved the way to studying gene function and developing cultivars with desirable fruit traits by overcoming long breeding cycles. Such comprehensive multi-omics approaches related to fruit characters in tropical fruits and their applications in breeding strategies and crop improvement are reviewed, discussed and presented here.

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Validation of genome-data based simple sequence repeats for ascertaining hybridity in mango (Mangifera indica) hybrids

MEGHA

Singh

Srivastav

et al. 2022

Indian J Agri Sci

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The present study was carried to identify polymorphic SSRs derived from the whole draft genome data of mango (Mangifera indica L.) for their polymorphism between Amrapali and Sensation genotypes (parents) and their validation on the derived hybrid progenies. Fifty new hypervariable mango SSRs (HMSSRs) were screened against the parental genotypes. Out of which, 11 HMSSRs were polymorphic, 18 were monomorphic and 21 did not amplify at all. These polymorphic HMSSRs were then used for ascertaining the hybridity of the hybrid progenies. The genetic diversity indices of each SSR locus among mango hybrids were calculated using Power Marker ver. 3.25. The genetic diversity indices such as the number of alleles ranged from 2 (HMSSR35) to 5 (HMSSR57), gene diversity from 0.36 (HMSSR54) to 0.71 (HMSSR57 & HMSSR58), and PIC values from 0.35 (HMSSR69) to 0.66 (HMSSR57). The dendrogram generated using Neighbor joining tree divided the 22 genotypes into three distinct clades, viz. Clade ‘A’, Clade ‘B’ and Clade ‘C’. The hybrids, viz. H-3-1, H-3-2, and H-7-4 were identified as true hybrids, since they were genetically more close to female parent (Amrapali), while hybrids H-3-3, H-3-11, H-11-1, H-12-5, H-1-6, H-1-13, H-12-8, H-12-10, H-3-12, H-1-1 and H-4-10 were more close to the male donor parent (Sensation) and also had red peel colour. In the present study, the 20 hybrids were confirmed to be the true hybrids since the bands unique to the two parents were present in them.

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MICROSATELLITE MARKERS FOR THE MOLECULAR CHARACTERISATION OF POTENTIALLY COMMERCIAL MANGO (Mangifera indica) PROGENIES

Cited by 3 publications

References 13 publications

Application of handheld NIR spectrometer for simultaneous identification and quantification of quality parameters in intact mango fruits

Application of handheld NIR spectrometer for simultaneous identification and quantification of quality parameters in intact mango fruits

Genomic Approaches for Improvement of Tropical Fruits: Fruit Quality, Shelf Life and Nutrient Content

Validation of genome-data based simple sequence repeats for ascertaining hybridity in mango (Mangifera indica) hybrids

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