Microsatellite markers for the European tree frog <i>Hyla arborea</i>

Arens, Paul; Westende, Wendy van’t; Bugter, Rob; Smulders, M.J.M.; Vosman, Ben

doi:10.1046/j.1365-294x.2000.01095-15.x

Cited by 45 publications

(44 citation statements)

References 5 publications

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“…Allelic frequencies were assessed at 15 autosomal microsatellite loci (Ha-A11, Ha-A119, Ha-A127, Ha-A130, Ha-B5R3, Ha-D3R3, Ha-D115, WHA1-20, WHA1-25, WHA1-67, WHA1-103, Ha-D-104, Ha-D-106 and Ha-H-116, Ha-A-136; Arens et al, 2000;Berset-Brändli et al, 2008a,b). Several measures of heterozygosity exist but one or the other are often used without theoretical justification.…”

Section: Genetic Analysesmentioning

confidence: 99%

Within- and among-population impact of genetic erosion on adult fitness-related traits in the European tree frog Hyla arborea

Luquet

David

et al. 2012

Heredity

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Assessing in wild populations how fitness is impacted by inbreeding and genetic drift is a major goal for conservation biology. An approach to measure the detrimental effects of inbreeding on fitness is to estimate correlations between molecular variation and phenotypic performances within and among populations. Our study investigated the effect of individual multilocus heterozygosity on body size, body condition and reproductive investment of males (that is, chorus attendance) and females (that is, clutch mass and egg size) in both small fragmented and large non-fragmented populations of European tree frog (Hyla arborea). Because adult size and/or condition and reproductive investment are usually related, genetic erosion may have detrimental effects directly on reproductive investment, and also on individual body size and condition that in turn may affect reproductive investment. We confirmed that the reproductive investment was highly size-dependent for both sexes. Larger females invested more in offspring production, and larger males attended the chorus in the pond more often. Our results did not provide evidence for a decline in body size, condition and reproductive effort with decreased multilocus heterozygosity both within and among populations. We showed that the lack of heterozygosity-fitness correlations within populations probably resulted from low inbreeding levels (inferior to ca. 20% full-sib mating rate), even in the small fragmented populations. The detrimental effects of fixation load were either low in adults or hidden by environmental variation among populations. These findings will be useful to design specific management actions to improve population persistence.

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Section: Genetic Analysesmentioning

confidence: 99%

Within- and among-population impact of genetic erosion on adult fitness-related traits in the European tree frog Hyla arborea

Luquet

David

et al. 2012

Heredity

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“…This method has been applied successfully to a number of plant (Van der Schoot et al 2000;Smulders et al 2001) and animal (Arens et al 2000) species. Sonificated DNA was used for enrichment of microsatellites using mixtures of synthetic oligonucleotides: (GT) 12 , (GA) 12 , and (TGT) 9 (pool1); (TCT) 10 , (TGTT) 8 , and (GTAT) 8 (pool 2); and (GAG) 8 , (GTG) 8 , and (GATA) 8 (pool 3).…”

Section: Microsatellite Isolationmentioning

confidence: 99%

Identification of cut rose (Rosa hybrida) and rootstock varieties using robust sequence tagged microsatellite site markers

Esselink¹,

Smulders²,

Vosman³

2003

Theor Appl Genet

138

149

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In this study a DNA fingerprinting protocol was developed for the identification of rose varieties based on the variability of microsatellites. Microsatellites were isolated from Rosa hybrida L. using enriched small insert libraries. In total 24 polymorphic sequenced tagged microsatellite site (STMS) markers with easily scorable allele profiles, from six different linkage groups, were used to characterize 46 Hybrid Tea varieties and 30 rootstock varieties belonging to different species (Rosa canina L., Rosa indica Thory., Rosa chinensis Jacq., Rosa rubiginosa L., and Rosa rubrifolia glauca Pour.). Clones and known flower color mutants were identified as being identical, all other varieties were differentiated by a unique pattern with as few as three STMS markers. The high discriminating power of the loci suggests that a selection of the most-robust STMS markers may be able to differentiate any two varieties within rootstocks or Hybrid Teas except for mutants. The selected STMS markers will be useful as a tool for reference collection management, for assessing essential derivation of varieties and illegal propagation.

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“…Mean H E ranged from 0.088-0.77 in studies reviewed by Newman and Squire (2001). More recently, mean H E was calculated from microsatellite loci in Hyla arborea (0.52, Arens et al 2000), Rana lessonae (0.57, Garner et al 2000;Zeisset et al 2000), R. ridibunda (0.59. Zeisset et al 2000), and R. temporaria (0.76, Berlin et al 2000).…”

Section: Genetic Diversitymentioning

confidence: 99%

Effective Population Sizes and Temporal Stability of Genetic Structure in Rana Pipiens, the Northern Leopard Frog

2004

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Abstract. Although studies of population genetic structure are very common, whether genetic structure is stable over time has been assessed for very few taxa. The question of stability over time is particularly interesting for frogs because it is not clear to what extent frogs exist in dynamic metapopulations with frequent extinction and recolonization, or in stable patches at equilibrium between drift and gene flow. In this study we collected tissue samples from the same five populations of leopard frogs, Rana pipiens, over a 22-30 year time interval (11-15 generations). Genetic structure among the populations was very stable, suggesting that these populations were not undergoing frequent extinction and colonization. We also estimated the effective size of each population from the change in allele frequencies over time. There exist few estimates of effective size for frog populations, but the data available suggest that ranid frogs may have much larger ratios of effective size (N e ) to census size (N c ) than toads (bufonidae). Our results indicate that R. pipiens populations have effective sizes on the order of hundreds to at most a few thousand frogs, and N e /N c ratios in the range of 0.1-1.0. These estimates of N e /N c are consistent with those estimated for other Rana species. Finally, we compared the results of three temporal methods for estimating N e . Moment and pseudolikelihood methods that assume a closed population gave the most similar point estimates, although the moment estimates were consistently two to four times larger. Wang and Whitlock's new method that jointly estimates N e and the rate of immigration into a population (m) gave much smaller estimates of N e and implausibly large estimates of m. This method requires knowing allele frequencies in the source of immigrants, but was thought to be insensitive to inexact estimates. In our case the method may have failed because we did not know the true source of immigrants for each population. The method may be more sensitive to choice of source frequencies than was previously appreciated, and so should be used with caution if the most likely source of immigrants cannot be identified clearly.Key words. Anuran, genetic structure, microsatellite, Rana pipiens, temporal stability, temporal variation, variance effective size. A fundamental goal of population genetics is to understand the relative importance of microevolutionary forces in determining the existing patterns of genetic variation within a species. Consequently, using molecular markers to estimate parameters such as the effective sizes of, and migration rates among, natural populations has become a major focus in the field of evolutionary biology. However, elucidation of past processes and the prediction of future patterns from molecular data requires that a state of equilibrium have developed between genetic drift and gene flow in the populations of interest. Demographic instability can disrupt that equilibrium (Whitlock 1992), rendering single ''snapshot'' estimates of population struc...

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Microsatellite markers for the European tree frog Hyla arborea

Cited by 45 publications

References 5 publications

Within- and among-population impact of genetic erosion on adult fitness-related traits in the European tree frog Hyla arborea

Within- and among-population impact of genetic erosion on adult fitness-related traits in the European tree frog Hyla arborea

Identification of cut rose (Rosa hybrida) and rootstock varieties using robust sequence tagged microsatellite site markers

Effective Population Sizes and Temporal Stability of Genetic Structure in Rana Pipiens, the Northern Leopard Frog

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