Microsatellite markers for the endangered European mink (<i>Mustela lutreola</i>) and closely related mustelids

Cabria, María Teresa; González, Elena; Gómez‐Moliner, Benjamín J.; Zardoya, Rafael

doi:10.1111/j.1471-8286.2007.01825.x

Cited by 21 publications

(16 citation statements)

References 9 publications

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“…A total of 11 polymorphic microsatellite loci were analyzed. Five specific loci (Mlut04, Mlut20, Mlut25, Mlut32 and Mlut35) were isolated directly from Mustela lutreola [ 12 ], whereas the rest were originally developed for Mustela erminea (Mer09, Mer22 and Mer41) and Neovison vison (Mvis22, Mvis72, Mvis75) [ 49 ]. The PCR conditions used are provided in Cabria et al .…”

Section: Methodsmentioning

confidence: 99%

“…In the present study, we combined molecular data (both mtDNA and nuclear microsatellites) from Michaux et al [ 10 ] with new molecular data obtained from additional specimens from Russia, Belarus, Romania, and Spain. We also used five new species-specific microsatellite loci isolated from European mink [ 12 ] on all samples. With these new data and recently developed Bayesian analysis methods, our goals were to gain further insight into: i) the current genetic structure of the European mink; ii) the phylogeographic processes associated with the glacial refuge and post-glacial colonization of the species, and the origin of the W population; iii) the role of river drainages in shaping the current species distribution; and iv) the existence of population genetic bottlenecks caused by the effects of human impact.…”

Section: Introductionmentioning

confidence: 99%

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Patterns of genetic variation in the endangered European mink (Mustela lutreola L., 1761)

et al. 2015

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BackgroundThe European mink (Mustela lutreola, L. 1761) is a critically endangered mustelid, which inhabits several main river drainages in Europe. Here, we assess the genetic variation of existing populations of this species, including new sampling sites and additional molecular markers (newly developed microsatellite loci specific to European mink) as compared to previous studies. Probabilistic analyses were used to examine genetic structure within and between existing populations, and to infer phylogeographic processes and past demography.ResultsAccording to both mitochondrial and nuclear microsatellite markers, Northeastern (Russia, Estonia and Belarus) and Southeastern (Romania) European populations showed the highest intraspecific diversity. In contrast, Western European (France and Spain) populations were the least polymorphic, featuring a unique mitochondrial DNA haplotype. The high differentiation values detected between Eastern and Western European populations could be the result of genetic drift in the latter due to population isolation and reduction. Genetic differences among populations were further supported by Bayesian clustering and two main groups were confirmed (Eastern vs. Western Europe) along with two contained subgroups at a more local scale (Northeastern vs. Southeastern Europe; France vs. Spain).ConclusionsGenetic data and performed analyses support a historical scenario of stable European mink populations, not affected by Quaternary climate oscillations in the Late Pleistocene, and posterior expansion events following river connections in both North- and Southeastern European populations. This suggests an eastern refuge during glacial maxima (as already proposed for boreal and continental species). In contrast, Western Europe was colonised more recently following either natural expansions or putative human introductions. Low levels of genetic diversity observed within each studied population suggest recent bottleneck events and stress the urgent need for conservation measures to counteract the demographic decline experienced by the European mink.Electronic supplementary materialThe online version of this article (doi:10.1186/s12862-015-0427-9) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Patterns of genetic variation in the endangered European mink (Mustela lutreola L., 1761)

et al. 2015

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“…Samples were individually genotyped with a novel multiplex panel of 23 autosomal microsatellite markers comprising 13 recently described species-specific microsatellites (Mf 1.1, Mf 1.11, Mf 1.18, Mf 1.3, Mf 2.13, Mf 3.2, Mf 3.7, Mf 4.10, Mf 4.17, Mf 6.5, Mf 8.7, Mf 8.8, Mf 8.10; [ 45 ]) and 10 additional markers described in closely related mustelids (Ma1 and Ma2 [ 46 ]; Mel1 [ 47 ]; MLUT27 [ 48 ]; Mvis072 [ 49 ]; Mvi57 [ 50 ]; Lut615 [ 51 ]; MP0059 [ 52 ]; and Lut435 and Lut453 [ 53 ]). The forward primers labelled with the dyes 6-FAM, NED, PET and VIC, were used in four PCR multiplex reactions (multiplex A, B, C and D; S2 Table ).…”

Section: Methodsmentioning

confidence: 99%

Inferring Population Genetic Structure in Widely and Continuously Distributed Carnivores: The Stone Marten (Martes foina) as a Case Study

et al. 2015

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The stone marten is a widely distributed mustelid in the Palaearctic region that exhibits variable habitat preferences in different parts of its range. The species is a Holocene immigrant from southwest Asia which, according to fossil remains, followed the expansion of the Neolithic farming cultures into Europe and possibly colonized the Iberian Peninsula during the Early Neolithic (ca. 7,000 years BP). However, the population genetic structure and historical biogeography of this generalist carnivore remains essentially unknown. In this study we have combined mitochondrial DNA (mtDNA) sequencing (621 bp) and microsatellite genotyping (23 polymorphic markers) to infer the population genetic structure of the stone marten within the Iberian Peninsula. The mtDNA data revealed low haplotype and nucleotide diversities and a lack of phylogeographic structure, most likely due to a recent colonization of the Iberian Peninsula by a few mtDNA lineages during the Early Neolithic. The microsatellite data set was analysed with a) spatial and non-spatial Bayesian individual-based clustering (IBC) approaches (STRUCTURE, TESS, BAPS and GENELAND), and b) multivariate methods [discriminant analysis of principal components (DAPC) and spatial principal component analysis (sPCA)]. Additionally, because isolation by distance (IBD) is a common spatial genetic pattern in mobile and continuously distributed species and it may represent a challenge to the performance of the above methods, the microsatellite data set was tested for its presence. Overall, the genetic structure of the stone marten in the Iberian Peninsula was characterized by a NE-SW spatial pattern of IBD, and this may explain the observed disagreement between clustering solutions obtained by the different IBC methods. However, there was significant indication for contemporary genetic structuring, albeit weak, into at least three different subpopulations. The detected subdivision could be attributed to the influence of the rivers Ebro, Tagus and Guadiana, suggesting that main watercourses in the Iberian Peninsula may act as semi-permeable barriers to gene flow in stone martens. To our knowledge, this is the first phylogeographic and population genetic study of the species at a broad regional scale. We also wanted to make the case for the importance and benefits of using and comparing multiple different clustering and multivariate methods in spatial genetic analyses of mobile and continuously distributed species.

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“…All 446 sampled individuals were genotyped using 13 microsatellite loci. Of these, six microsatellites (MLUT04, MLUT20, MLUT25, MLUT27, MLUT 32 and MLUT35) were isolated from the European mink (Cabria et al. 2007), whereas the remaining were originally developed for either the American mink, N. vison (MVIS22, MVIS72, MVIS75 and MVIS99) or the stoat, Mustela erminea (MER09, MER22 and MER41) (Fleming et al.…”

Section: Methodsmentioning

confidence: 99%

Bayesian analysis of hybridization and introgression between the endangered european mink (Mustela lutreola) and the polecat (Mustela putorius)

et al. 2011

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Human-mediated global change will probably increase the rates of natural hybridization and genetic introgression between closely related species, and this will have major implications for conservation of the taxa involved. In this study, we analyse both mitochondrial and nuclear data to characterize ongoing hybridization and genetic introgression between two sympatric sister species of mustelids, the endangered European mink (Mustela lutreola) and the more abundant polecat (M. putorius). A total of 317 European mink, 114 polecats and 15 putative hybrid individuals were collected from different localities in Europe and genotyped with 13 microsatellite nuclear markers. Recently developed Bayesian methods for assigning individuals to populations and identifying admixture proportions were applied to the genetic data. To identify the direction of hybridization, we additionally sequenced mtDNA and Y chromosomes from 78 individuals and 29 males respectively. We found that both hybridization and genetic introgression occurred at low levels (3% and 0.9% respectively) and indicated that hybridization is asymmetric, as only pure polecat males mate with pure European mink females. Furthermore, backcrossing and genetic introgression was detected only from female first-generation (F1) hybrids of European mink to polecats. This latter result implies that Haldane's rule may apply. Our results suggest that hybridization and genetic introgression between the two species should be considered a rather uncommon event. However, the current low densities of European mink might be changing this trend.

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Microsatellite markers for the endangered European mink (Mustela lutreola) and closely related mustelids

Cited by 21 publications

References 9 publications

Patterns of genetic variation in the endangered European mink (Mustela lutreola L., 1761)

Patterns of genetic variation in the endangered European mink (Mustela lutreola L., 1761)

Inferring Population Genetic Structure in Widely and Continuously Distributed Carnivores: The Stone Marten (Martes foina) as a Case Study

Bayesian analysis of hybridization and introgression between the endangered european mink (Mustela lutreola) and the polecat (Mustela putorius)

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