Microsatellite markers for European <i>Daphnia</i>

Brede, Nora; Thielsch, Anne; Sandrock, Christoph; Spaak, Piet; Keller, Barbara; Streit, Bruno; Schwenk, Klaus

doi:10.1111/j.1471-8286.2005.01218.x

Cited by 62 publications

(98 citation statements)

References 16 publications

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“…Furthermore, methods enabling indirect determination of Daphnia taxa are also available, especially the recently developed set of 32 microsatellite markers for the D. longispina complex (Brede et al, 2006). Hybridizing species of the complex can be well distinguished when several microsatellite loci are analyzed Thielsch et al, 2009), confirming the suitability of these markers for the identification of hybrid genotypes.…”

Section: Introductionmentioning

confidence: 86%

“…Amplification and length assessment of the fragments mostly followed the protocol by Brede et al (2006) and Thielsch et al (2009). After amplicons had been obtained, they were appropriately diluted and electrophoresed on a CEQ 2000 capillary sequencer (Beckman Coulter, Fullerton, CA, USA) with self-designed size standards based on Lambda virus DNA (Symonds & Lloyd, 2004).…”

Section: Microsatellite Analysismentioning

confidence: 99%

“…Here, we present a comparison of three molecular methods for genetic identification of common species and hybrids of the D. longispina complex (in particular, D. galeata, D. longispina and D. cucullata): allozyme electrophoresis (according to Wolf & Mort, 1986;Gießler, 1997), ITS-RFLP (according to Skage et al, 2007), and the analysis of 12 microsatellite markers (from Brede et al, 2006). In particular, we define the extent to which the results of DNAbased methods deviate from those of allozyme electrophoresis, as this method was employed in most studies on interspecific hybridization within the D. longispina complex.…”

Section: Introductionmentioning

confidence: 99%

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Identifying hybridizing taxa within the Daphnia longispina species complex: a comparison of genetic methods and phenotypic approaches

et al. 2010

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Daphnia galeata Sars, D. longispina O. F. Müller and D. cucullata Sars (Crustacea: Cladocera) are closely related species which often produce interspecific hybrids in natural populations. Several marker systems are available for taxon determination in this hybridizing complex, but their performance and reliability has not been systematically assessed. We compared results from identifications by three molecular methods. More than 1,200 individuals from 10 localities in the Czech Republic were identified as parental species or hybrids by allozyme electrophoresis and the analysis of the restriction fragment length polymorphism of the internal transcribed spacer (ITS-RFLP); over 440 of them were additionally analyzed and identified by 12 microsatellite loci. Identification by microsatellite markers corresponded well with allozyme analyses. However, consistent discrepancies between ITS-RFLP and other markers were observed in two out of 10 studied localities. Although some marker discrepancies may have been caused by occasional recent introgression, consistent deviations between ITS-RFLP and other markers suggest a long-term maintenance of introgressed alleles. These results warn against its use as a sole identification method in field studies. Additionally, we quantitatively evaluated the discriminatory power of geometric morphometric (elliptic Fourier) analysis of body shapes based on photos of over 1,300 individuals pre-classified by allozyme markers. Furthermore, a randomly selected subset of 240 individuals was independently determined from photos by several experts. Despite a tendency for morphological divergence among parental Daphnia species, some taxa (especially D. galeata, D. longispina, and their hybrids) substantially overlapped in their body shapes. This was reflected in different

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Section: Introductionmentioning

confidence: 86%

Section: Microsatellite Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identifying hybridizing taxa within the Daphnia longispina species complex: a comparison of genetic methods and phenotypic approaches

et al. 2010

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“…This technique has shown to be useful in studies on systematics and in the differentiation of strains and breeds within the same species (e.g., Alves et al, 2007) and within cell lineages from the same individual (e.g., Grasela and McIntosh, 2003). DNA was extracted individually from preserved specimens (in 70-80% ethanol) of each population using a standard protocol (Schwenk et al, 1998), which has undergone minor adjustments over time (see Brede et al, 2006). To maximize extraction, only adult females were used and a preliminary step consisting of specimen maceration was included in the extraction procedure.…”

Section: Methodsmentioning

confidence: 99%

Genetic variability in the tolerance of natural populations ofSimocephalus vetulus(Müller, 1776) to lethal levels of sodium chloride

Loureiro

Castro

Claro

et al. 2012

Ann. Limnol. - Int. J. Lim.

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-Using several clonal lineages of Simocephalus vetulus (Cladocera, Daphniidae) as a random sample, we investigated the genetic component of the halotolerance of one brackish and two freshwater populations of this littoral filter feeder. We hypothesized that genotypes from the brackish population were more tolerant than freshwater ones, via adaptation to local environmental conditions. Clonal identity was established by a cost-effective molecular fingerprinting technique (microsatellite-primed polymerase chain reaction (MSP-PCR)). Two distinct methodologies were used to assess cladoceran sensitivity to syntheticgrade sodium chloride (NaCl): (i) standard 48-h acute assays and (ii) 12-h survival time (ST) trials. No correlation was found between acute EC 50 and ST values. The sensitivity of brackish and freshwater clones was comparable in terms of acute EC 50 (varied from 2.28 to 3.83 g.L x1). On the contrary, genetically determined differential tolerance to NaCl among populations was found for ST: all brackish genotypes, except one, were more resilient (ST > 120 min) than freshwater clones (ST < 120 min). Bearing in mind that these results were obtained with isolates from the extant population, it is surprising that the range of acute sensitivity of the freshwater and brackish genotypes was similar, and that the only difference between them was the ability of brackish clones to survive longer under high salinity stress (6 g.L x1, in ST trials). We must conclude that the effect of salinity (original environment context) on the selection of genotypes was weaker than we had expected and than other authors have shown for other stressors.

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“…Specific banding patterns allow identification of the taxa: D. galeata (100, 320, 380 and 490 bp), D. hyalina (100, 520 and 680 bp) and their hybrid who displays an additive banding pattern in the RFLP analysis. ITS RFLP analyses were compared with microsatellite analyses (six loci: DaB 10/ 15, DaB 17/17, DaB 17/16, DaB 10/14, Dp512 and Dp519; see Brede et al, 2005) and resulted in similar taxon classifications.…”

Section: Genetic Analysismentioning

confidence: 99%

The contribution of differential hatching success to the fitness of species and interspecific hybrids

et al. 2007

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Resting egg banks of microcrustaceans have been used to reconstruct the evolutionary and ecological history of species. However, recent studies provided evidence for a discrepancy between dormant propagules in the sediment and the planktonic population. This pattern raises two questions: First, what is the value of data on resting egg banks for population dynamics over time and second, which component of the reproductive cycle causes the observed inconsistency? In our study we focussed on the second question by comparing the taxon composition of a resting egg bank with the reproductive success of ex-ephippial hatchlings. Species and interspecific hybrid identification of dormant and hatched stages was achieved through the application of restriction fragment length polymorphism analysis of an internal transcribed spacer region. We found no significant deviation between the proportion of hatched Daphnia galeata, D. galeata · hyalina and D. hyalina individuals and the observed taxon composition of the resting egg bank. However, species and hybrids differed in their mode and relative success of reproduction. We conclude that the components of reproductive success in Daphnia contribute differentially to the fitness of species and interspecific hybrids. The discrepancy between resting egg banks and ''active'' planktonic populations results not from differential hatching of species but from the reproductive success of ex-ephippial females and the timing and frequency of sexual reproduction of the different taxa.

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Microsatellite markers for European Daphnia

Cited by 62 publications

References 16 publications

Identifying hybridizing taxa within the Daphnia longispina species complex: a comparison of genetic methods and phenotypic approaches

Identifying hybridizing taxa within the Daphnia longispina species complex: a comparison of genetic methods and phenotypic approaches

Genetic variability in the tolerance of natural populations ofSimocephalus vetulus(Müller, 1776) to lethal levels of sodium chloride

The contribution of differential hatching success to the fitness of species and interspecific hybrids

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