2009
DOI: 10.1007/s10592-009-9904-2
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Microsatellite markers for Dictyochloropsis reticulata (Trebouxiophyceae), the symbiotic alga of the lichen Lobaria pulmonaria (L.)

Abstract: We isolated and characterized eight microsatellite markers for Dictyochloropsis reticulata, the primary photosynthetic partner of the epiphytic lichen Lobaria pulmonaria. These are the first microsatellite loci reported for a lichen symbiotic alga. These polymorphic markers will be useful for investigating spatial genetic structure, biogeography and dispersal of this eukaryotic alga and will generally shed light on the coevolution of the green-algal lichen symbioses.

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Cited by 28 publications
(30 citation statements)
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“…Eight fungal-specific and seven algal-specific microsatellite markers were amplified from total lichen DNA (for RTL, RVZ and RXS seven fungal specific and seven alga specific markers were used) [42], [47]–[49]. Fragment lengths were detected on a 3730xl DNA Analyzer (Life Technologies), and electropherograms were analyzed with GENEMAPPER 3.7 (Life Technologies) using LIZ500 as size standard.…”
Section: Methodsmentioning
confidence: 99%
“…Eight fungal-specific and seven algal-specific microsatellite markers were amplified from total lichen DNA (for RTL, RVZ and RXS seven fungal specific and seven alga specific markers were used) [42], [47]–[49]. Fragment lengths were detected on a 3730xl DNA Analyzer (Life Technologies), and electropherograms were analyzed with GENEMAPPER 3.7 (Life Technologies) using LIZ500 as size standard.…”
Section: Methodsmentioning
confidence: 99%
“…We also analyzed seven photobiont microsatellite loci (LPh1–LPh7) following Dal Grande et al [25]. We ran fragment analyses on an automated capillary sequencer (3130 Genetic Analyzer, Applied Biosystems, Rotkreuz, Switzerland), and typed alleles using an internal size standard (LIZ500, Applied Biosystems, Rotkreuz, Switzerland).…”
Section: Methodsmentioning
confidence: 99%
“…Microsatellites are most useful for comparing closely related individuals and populations, although they have also in some cases been used to study populations situated on different continents, on a phylogeographic scale. The first microsatellite markers reported for lichens were developed for the mycobiont of Lobaria pulmonaria ; more recently, this method was also adopted for investigation of its green algal photobiont, Dictyochloropsis reticulata (Dal Grande et al 2009, 2012Widmer et al 2012). They offered a significant advantage over previous markers in providing a means by which a large number of individuals could be genotyped at a moderate cost per individual and have been employed successfully in studying genetic differentiation between populations and small-scale geographic variability within populations.…”
Section: Experimental Approachesmentioning
confidence: 99%