Microsatellite loci for <i>Euglossa annectans</i> (Hymenoptera: Apidae) and their variability in other orchid bees

Paxton, Robert J.; Zobel, Marion U.; Steiner, Josefina; Zillikens, Anne

doi:10.1111/j.1755-0998.2009.02612.x

Cited by 25 publications

(27 citation statements)

References 10 publications

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“…The E. dilemma individuals for the genetic distance study were genotyped at three different microsatellite loci: ann02 (GenBank accession no. BV728898), ann08 (BV728902) (Paxton et al 2009) and Egc17 (EF451846) (Souza et al 2007). Fragment analyses of PCR products were carried out with an ABI Prism 310 TM Sequencer (PE Applied Biosystems) at the University of Düsseldorf (BMFZ).…”

Section: Dna Extraction and Microsatellite Analysismentioning

confidence: 98%

Population genetic structure of orchid bees (Euglossini) in anthropogenically altered landscapes

et al. 2011

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Habitat degradation and fragmentation are widespread phenomena in tropical regions. Negative effects on the biota are numerous, ranging from interruption of gene flow among populations, to the loss of genetic diversity within populations, to a decline in species richness over time. Orchid bees (Hymenoptera: Apidae: Euglossini) are of major conservation interest due to their function as pollinators of numerous orchid species and other tropical plants. Here, we used microsatellite markers to investigate the effects of geographic distance and habitat fragmentation on gene flow among populations. Populations of Euglossa dilemma in three geographic regionsthe Yucatán peninsula (Mexico), Veracruz (Mexico), and Florida (USA)-were genetically structured predominantly across the regions, with the strength of differentiation among populations being positively correlated with geographic distance. Within geographic regions only little substructure was found, suggesting that dispersal is substantial in the absence of geographic or ecological barriers. In a second study, patterns of genetic differentiation among eight species of Euglossa were not related to habitat fragmentation following deforestation in southern Mexico (Veracruz). Specifically, most bee populations in the 9,800 ha forest remnant of Los Tuxtlas (Volcano San Martin) were neither differentiated from, nor had less genetic diversity than, populations in near-continuous forest separated from Los Tuxtlas by 130 km of agricultural land. Either occasional long distance dispersal across open areas has buffered the expected genetic effects of fragmentation, or the history of fragmentation in southern Mexico is too recent to have caused measurable shifts in allelic composition.

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Section: Dna Extraction and Microsatellite Analysismentioning

confidence: 98%

Population genetic structure of orchid bees (Euglossini) in anthropogenically altered landscapes

et al. 2011

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“…bombiformis males at 9 microsatellite loci: Eln2J, Eln5J, Eln7J, Eln10J, Eln 12a, Eln 12b, Eln 12J, Eln18J, Eln19J (Souza et al 2007); and genotyped the Eug. championi males at 10 microsatellite loci: Egc 17, Egc 18, Egc 24, Egc 26, Egc 30a, Egc 30b, Egc 35, Egc 37, Egc 51 (Souza et al 2007), and ann28 (Paxton et al 2009). Forward primers were labeled with fluorescent dyes (Applied Biosystems).…”

Section: Molecular Protocolmentioning

confidence: 99%

Population genetics of orchid bees in a fragmented tropical landscape

Suni

Brosi

2011

Conserv Genet

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The prospects for persistence of bees living in fragmented landscapes is a topic of considerable interest due to bees' importance as pollinators of agricultural crops and wild plants, coupled with the ubiquity of native habitat loss and evidence that bees may be declining worldwide. Population persistence in fragmented areas depends on dispersal potential and maintenance of gene flow among fragments of habitat. Here we used population genetic techniques to characterize, for two equally abundant orchid bee species that differ in their physiology and ecology, levels of genetic differentiation among fragments of tropical forest in southeastern Costa Rica in a *200 km 2 landscape. We measured population differentiation with / PT (an analogue to the traditional summary statistic Fst), as well as two measures that may more accurately reflect the level of differentiation when highly variable loci are used: G'st and D est . We also calculated pairwise genetic distances among individuals and conducted Mantel tests to test the correlation of genetic and geographic distance, for each species. We found strong differences in genetic structure between the species. Contrary to our expectations, each measure of genetic structure revealed that the largerbodied species, Eulaema bombiformis, had higher levels of differentiation than the smaller species, Euglossa championi. Furthermore, for Eulaema bombiformis there was a significant positive correlation of genetic and geographic distance while for Euglossa championi there was no significant positive correlation. Our results demonstrate that bee species can have strikingly different levels of gene flow in fragmented habitats, and that body size may not always act as a useful proxy for dispersal, even in closely related taxa.

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“…One of the causes for an increase of diploid males in populations of Euglossini and other groups of bees is the reduction in population size, with consequent loss of allelic diversity at the sex locus (Cook 1993;Roubik et al 1996;Zayed et al 2004;van Wilgenburg et al 2006;Paxton et al 2009). Thus, apparently, except for Eg.…”

Section: Discussion/ Conclusionmentioning

confidence: 99%

“…pleosticta and Eg. annectans were based on protocols of Souza et al (2007), Paxton et al (2009) andFreiria et al (2012), with some modifications. PCR reactions were produced in a final volume of 5 μL, including: 1x GoTaq® Colorless Master Mix 2x (Promega), 0.5 μM of tag-F primer+0.5 μM of R-primer mixture, 5 μM M13-primer labelled with fluorochrome, 1 % of glycerol, 5 ng of DNA and 1.15 μL nuclease-free water (Promega).…”

Section: Dna Extraction and Molecular Analysismentioning

confidence: 99%

Orchid bees: a new assessment on the rarity of diploid males in populations of this group of Neotropical pollinators

et al. 2015

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-The incidence of diploid males among 1457 individuals of different populations of six euglossine bee species (Eufriesea violacea , Eulaema cingulata , Euglossa annectans , Euglossa iopoecila , Euglossa pleosticta and Euglossa truncata ) was investigated with microsatellite markers. Bees were surveyed in Atlantic Forest fragments showing different sizes and degrees of human interference. Our analyses revealed that, although the frequencies of diploid males remained below 10 % for five out of six species studied, the frequencies of these males in three populations of Eg. annectans were above 15.0 %. For this species, while the average estimate of diploid males was around 11.0 %, the highest percentage (27 %) of such males was found for the population from an island. Our findings indicate that the frequency of diploid males can vary substantially among species and this fact should be taking into consideration in studies and conservation actions involving different euglossine bees. microsatellites / Euglossini / euglossine bees / Euglossa annectans / forest fragments

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Microsatellite loci for Euglossa annectans (Hymenoptera: Apidae) and their variability in other orchid bees

Cited by 25 publications

References 10 publications

Population genetic structure of orchid bees (Euglossini) in anthropogenically altered landscapes

Population genetic structure of orchid bees (Euglossini) in anthropogenically altered landscapes

Population genetics of orchid bees in a fragmented tropical landscape

Orchid bees: a new assessment on the rarity of diploid males in populations of this group of Neotropical pollinators

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