Microsatellite Genotyping of Candida parapsilosis Clinical Isolates

Abstract

Background and Purpose: Candida parapsilosis is a predominant species found in nosocomial infection, particularly in hospitalized patients. The molecular epidemiology of the clinical strains of this species has not been well studied. The present study was performed with the aim of investigating the microsatellite genotyping of Candida parapsilosis among the Iranian clinical isolates.Materials and Methods:This study was conducted on 81 independent clinical C. parapsilosis isolates that were genotyped by using a panel of six microsatellite markers.Results:The short tandem repeat (STR) typing of clinical C. parapsilosis isolates demonstrated 68 separate genotypes, among which 57 genotypes were observed once and the remaining 11 cases were identified for multiple times. The Simpson’s diversity index for the panel of combined six markers yielded a diversity index of 0.9951. The heterogeneity was observed among the Iranian and the Netherlands clinical C. parapsilosis isolates.Conclusion:As the findings indicated, the clinical C. parapsilosis isolates from Iran showed a high genetic diversity. It can be concluded that molecular epidemiology could be useful for screening during outbreak investigation where C. parapsilosis is involved.

Molecular Characterization of Candida parapsilosis by Microsatellite Typing and Emergence of Clonal Antifungal Drug Resistant Strains in a Multicenter Surveillance in China

“…In the present study, we used microsatellite genotyping method to study the molecular epidemiology of C. parapsilosis, cultured from patients with invasive fungal infections, as part of the national surveillance program CHIF-NET 16-17. We chose to use microsatellite typing as this method has emerged as a powerful tool to study the genetic relationship between C. parapsilosis isolates in the outbreak setting (Vaz et al, 2011;Diab-Elschahawi et al, 2012;Pulcrano et al, 2012;Reiss et al, 2012;Romeo et al, 2013;Delfino et al, 2014;Sabino et al, 2015;Wang et al, 2016;Badali et al, 2017;Magobo et al, 2017;Desnos-Ollivier et al, 2018).…”

Investigation of a candidemia outbreak caused by C. parapsilosis in a pediatric hematology/oncology center

“…В связи с этим интересна возможность идентификации отдельных штаммов в рамках эпидеми ологических расследований. Один из наиболее точных и распространенных методов, используемых для иден тификации штаммов C. рarapsilosis, -генотипирование мик росателлитов (STRмаркеров) [4][5][6][7]. Метод осно ван на амплификации нескольких коротких тандемных повторов, расположенных в разных геномных локусах.…”