Microsatellite DNA markers of Ezo abalone (Haliotis discus hannai): a preliminary assessment of natural populations sampled from heavily stocked areas

Sekino, Masashi; Saido, Takahiro; Fujita, Tsuneo; Kobayashi, Toshimasa; Takami, Hideki

doi:10.1016/j.aquaculture.2004.10.013

Cited by 45 publications

(30 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Microsatellite analysis: The source of microsatellite markers used in this study was as follows: two markers suffixed with Hdd in Sekino and Hara (2001), six markers with Hd in Hara and Sekino (2005), 111 markers with Afa and Awb in Sekino et al (2005Sekino et al ( , 2006, and 15 markers with Ahdh in Sekino and Hara (2007). These markers turned out to be informative in at least one of the three mapping families on the basis of our preliminary marker screening.…”

Section: Methodsmentioning

confidence: 99%

“…In both cases, Cy5-fluorescent dye was conjugated to the 59 end of the primers (Sigma Genosys, Hokkaido, Japan) so that amplified alleles could be detected on an ALFexpress/ALFexpress II DNA sequencer (GE Healthcare Bio-Sciences, Piscataway, NJ). PCR assays were performed as described in Sekino et al (2005) for the dye-primer method and in Sekino et al (2006) for the 59-tailed primer method.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Linkage Maps for the Pacific Abalone (Genus Haliotis) Based on Microsatellite DNA Markers

Sekino

Hara

2007

Genetics

Self Cite

View full text Add to dashboard Cite

This study presents linkage maps for the Pacific abalone (Haliotis discus hannai) based on 180 microsatellite DNA markers. Linkage mapping was performed using three F 1 outbred families, and a composite linkage map for each sex was generated by incorporating map information from the multiple families. A total of 160 markers are placed on the consolidated female map and 167 markers on the male map. The numbers of linkage groups in the composite female and male maps are 19 and 18, respectively; however, by aligning the two maps, 18 linkage groups are formed, which are consistent with the haploid chromosome number of H. discus hannai. The female map spans 888.1 cM (Kosambi) with an average spacing of 6.3 cM; the male map spans 702.4 cM with an average spacing of 4.7 cM. However, we encountered several linkage groups that show a high level of heterogeneity in recombination rate between families even within the same sex, which reduces the precision of the consolidated maps. Nevertheless, we suggest that the composite maps are of significant potential use as a scaffold to further extend the coverage of the H. discus hannai genome with additional markers.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Linkage Maps for the Pacific Abalone (Genus Haliotis) Based on Microsatellite DNA Markers

Sekino

Hara

2007

Genetics

Self Cite

View full text Add to dashboard Cite

show abstract

“…The Pacific abalone (Haliotis discus), which distributes along the coasts of East Asia, is one of the most important species in both fisheries and aquaculture (Wan et al, 2004;Sekino et al, 2005). Due to its high economic values and great potential demands in markets, a series of breeding programs have been carried out (Wan et al, 2004;Sekino et al, 2005) and stock enhancement attempts have been made. In order to answer questions at the levels of individual, population and species, microsatellite markers should be developed due to their preeminent advantages, such as co-dominance, high polymorphism and even distribution in the genome.…”

Section: Introductionmentioning

confidence: 99%

Development and characterization of microsatellite markers for the Pacific abalone (Haliotis discus) via EST database mining

Zhan

Bao

Wang

et al. 2008

J. Ocean Univ. China

View full text Add to dashboard Cite

The EST database of the Pacific abalone (Haliotis discus) was mined for developing microsatellite markers. A total of 1476 EST sequences were registered in GenBank when data mining was performed. Fifty sequences (approximately 3.4%) were found to contain one or more microsatellites. Based on the length and GC content of the flanking regions, cluster analysis and BLASTN, 13 microsatellite-containing ESTs were selected for PCR primer design. The results showed that 10 out of 13 primer pairs could amplify scorable PCR products and showed polymorphism. The number of alleles ranged from 2 to 13 and the values of H o and H e varied from 0.1222 to 0.8611 and 0.2449 to 0.9311, respectively. No significant linkage disequilibrium (LD) between any pairs of these loci was found, and 6 of 10 loci conformed to the Hardy-Weinberg equilibrium (HWE). These EST-SSRs are therefore potential tools for studies of intraspecies variation and hybrid identification.

show abstract

“…PCR examination and allele scoring was carried out according to Hara and Sekino (2005) and Sekino et al (2005). Primer sequences and annealing temperature of each primer set optimized for H. discus hannai are shown in Table 1.…”

Section: Abalone Samples and Dna Experimentsmentioning

confidence: 99%

Individual assignment tests proved genetic boundaries in a species complex of Pacific abalone (genus Haliotis)

Sekino

Hara

2006

Conserv Genet

View full text Add to dashboard Cite

Abstracts We conducted this study to find genetic evidence to distinguish the members of Pacific abalone species complex (Haliotis discus hannai, H. discus discus, H. madaka, and H. gigantea) based on microsatellite DNA markers, illustrating the potential of microsatellites for species-assignment. First, we addressed the transferability of H. discus hannai microsatellites to the three other members of Pacific abalone and five additional species (H. diversicolor aquatilis, H. midae, H. corrugata, H. fulgens, and H. rubra). Second, using the microsatellites we applied two types of individual assignment testing (the distance-based assignment and Bayesian modelbased clustering) to individuals from the Pacific abalone species. A total of 24 microsatellites were subjected to PCR trials for nine Haliotis species, and the cross-species amplification performance of these markers turned out to drop precipitously even for less divergent congeners. Within the Pacific abalone species complex, four of the 24 markers were not transferable to H. gigantea, suggesting a solid genetic boundary between H. gigantea and H. discus hannai, H. discus discus, and H. madaka. Among the three latter abalones, both assignment tests achieved approximately 90% or more success rate of assignment. The feasibility of the microsatellite markers to classify species sheds light on the genetic management of the Pacific abalone species complex.

show abstract

Microsatellite DNA markers of Ezo abalone (Haliotis discus hannai): a preliminary assessment of natural populations sampled from heavily stocked areas

Cited by 45 publications

References 46 publications

Linkage Maps for the Pacific Abalone (Genus Haliotis) Based on Microsatellite DNA Markers

Linkage Maps for the Pacific Abalone (Genus Haliotis) Based on Microsatellite DNA Markers

Development and characterization of microsatellite markers for the Pacific abalone (Haliotis discus) via EST database mining

Individual assignment tests proved genetic boundaries in a species complex of Pacific abalone (genus Haliotis)

Contact Info

Product

Resources

About