Microsatellite DNA markers for <i>Podocnemis unifilis</i>, the endangered yellow‐spotted Amazon River turtle

Fantin, Cleiton; Carvalho, Cecília F.; Hrbek, Tomas; Sites, Jack W.; Monjeló, Luiz Alberto Santos; Astolfi-Filho, Spártaco; Farias, Izeni Pires

doi:10.1111/j.1471-8286.2007.01842.x

Cited by 16 publications

(13 citation statements)

References 8 publications

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“…The number of homozygote genotypes observed in the nestlings was important to infer the maternal genotype for some of the nests, and also to estimate how many adult individuals could be contributing genetically to each nest. Fantin et al, 2007). H E = expected heterozygosity; Q = probability of paternity exclusion; I = probability of genetic identity; Joint probability of paternal exclusion at all loci (QC); Joint probability of genetic identity at all loci (IC).…”

Section: Resultsmentioning

confidence: 99%

“…To assess paternity, we chose eight microsatellite loci from those characterized by Fantin et al (2007) for P. unifilis that gave us best potential for relatedness analysis based on the estimated of the probability of paternity exclusion at an individual locus (Q or Pei), the total probability of paternal exclusion at all loci (QC or P et ) following Weir (1996) the estimated probability of genetic identity at an individual locus (I ), and the joint probability of genetic identity at all loci (IC), according to Paetkau et al (1995) (table 1). Total genomic DNA was extracted using GFX DNA extraction kit (GE-Healthcare).…”

Section: Methodsmentioning

confidence: 99%

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Polyandry in Podocnemis unifilis (Pleurodira; Podocnemididae), the vulnerable yellow-spotted Amazon River turtle

et al. 2008

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We used eight microsatellite loci to investigate the mating system in P. unifilis. DNA samples were collected from 66 hatchlings sampled from six nests on a central Amazon River beach near Barreirinha, Amazonas, Brazil. We found that P. unifilis females exhibited a promiscuous mating system (polyandry) with all nests being fathered by at least two males. The presence of multiple males contributing to each clutch was ascertained by the presence of at least two extra alleles in multilocus genotypes in each nest. Our data demonstrate that polyandry seems to be the prevalent system of mating in this endangered species.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Polyandry in Podocnemis unifilis (Pleurodira; Podocnemididae), the vulnerable yellow-spotted Amazon River turtle

et al. 2008

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“…Genomic DNA of each individual was amplified by polymerase chain reaction (PCR) using the technique of Schuelke (2000). Of the four microsatellite primers used in PCR, two were devel-oped by Fantin et al (2007) (Puni_1D9 and Puni_1E1), and two by Valenzuela (2000) (PE344 and PE519). The PCR products were diluted in a proportion of 1:100, and the size marker ROX pUC-19, modified from Dewoody et al (2004), was added to determine sizes of observed alleles.…”

Section: Methodsmentioning

confidence: 99%

Short Communication Polyandry in the red-headed river turtle Podocnemis erythrocephala (Testudines, Podocnemididae) in the Brazilian Amazon

Fantin¹,

Farias²,

Monjeló³

et al. 2010

Genet. Mol. Res.

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ABSTRACT. The genus Podocnemis comprises six living species, including P. erythrocephala (irapuca -red-headed river turtle). Data are available concerning the reproductive biology of the species of the genus Podocnemis, but little is known about their reproductive strategies. Considering the total lack of such data for P. erythrocephala, and with the goal of contributing information on their mode of reproduction, we examined the relationships among individuals of nests of this turtle, using microsatellite markers. Using four microsatellite loci, we analyzed the progeny in six nests from two localities in the Brazilian Amazon (Santa Isabel do rio Negro and Parintins). All juveniles from each nest were analyzed. The genotypes of each juvenile from each nest were identified, and because a sample of female DNA was not available, the maternal genotype was inferred from homozygous individuals in each nest. We found that this species is promiscuous; there was multiple paternity in five of the six nests analyzed. In addition to being important for the understanding of evolutionary and genetic processes, this type of information will be useful for chelonian management projects. Our data suggest one possible difference between reproductive patterns of the different populations. This multi-paternal condition may be a natural reproductive strategy for the preservation of the genetic diversity of this species.

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“…Genomic DNA was extracted using a Qiagen DNA blood extraction kit (Qiagen Benelux B.V., Venlo, The Netherlands), following the manufacturer's instructions. Polymerase Chain Reaction (PCR) with fluorescently labelled primers (6-FAM, HEX, PET, NED, Biomers, Ulm, Germany) was used to individually amplify 13 microsatellites originally designed for P. unifilis (Fantin et al 2007) and five additional ones for P. expansa (Sites et al 1999;Valenzuela 2000). Seven markers for P. unifilis (Puni1B2, Puni1B10, Puni1B11, Puni1F10, Puni2A9, Puni2C11, Puni2D9, Fantin et al 2007) and three for P. expansa (Sat62, Sat128, Sites et al 1999;PE519, Valenzuela 2000) proved to be useful for P. lewyana by successful cross-amplification and appropriate levels of polymorphism; the presence of microsatellite repeats was confirmed by sequencing on an ABI 3130xl Genetic Analyzer (Applied Biosystems, Foster City, CA, USA).…”

Section: Sampling and Laboratory Proceduresmentioning

confidence: 99%

Extremely low genetic diversity and weak population differentiation in the endangered Colombian river turtle Podocnemis lewyana (Testudines: Podocnemididae)

Vargas‐Ramírez¹,

Stuckas²,

Castaño-Mora

et al. 2011

Conserv Genet

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Podocnemis lewyana is an endangered endemic river turtle of Colombia. Using ten unlinked polymorphic microsatellite loci and a 691-bp-long DNA fragment corresponding to the more variable portion of the mitochondrial control region, we investigated genetic diversity and population structure throughout its range. Both neutral markers showed extremely low diversity and weak population differentiation. Our data indicate that the genetic history of P. lewyana has been impacted by multiple bottlenecks and population expansion since the Pleistocene. The observed differentiation pattern is most likely the result of historically low genetic variation resulting from restricted geographic range and aggravated by recent anthropogenically induced bottlenecks. Based on slight differences in allele frequencies among populations, we suggest that three regions should be treated as demographically independent Management Units in order to preserve maximal genetic diversity: (1) the Upper Magdalena River Basin, (2) the Lower Magdalena ? Lower Cauca ? San Jorge River Basins, and (3) the Sinú River Basin. Among the Management Units, only low to moderate levels of gene flow were detected; these are largely unidirectional from Management Units 1 and 3 into Management Unit 2.Resumen Podocnemis lewyana es una tortuga endémica y amenazada de Colombia. Usando diez loci polimórficos de microsatélites y un fragmento de 691 pares de bases correspondiente a la porción más variable de la región control mitocondrial, investigamos la diversidad genética y estructura poblacional a través de su rango de distribución. Ambos marcadores neutrales revelaron una diversidad extremadamente baja y débil diferenciación poblacional. Nuestros datos indican que la historia genética de P. lewyana ha sido impactada por múltiples cuellos de botella y expansión poblacional desde el Pleistoceno. El patrón de diferenciación observado es más probable el resultado de la variación genética historica baja derivada del rango de distribución restringido, agravada por cuellos de botella recientes resultado de intervención antrópica. Basados en diferencias tenues de frecuencias alélicas entre poblaciones, sugerimos que tres regiones deben ser tratadas como Unidades de Manejo (UM) demográficamente independientes con el fin de preservar al máximo la diversidad genética. (1) Cuenca del alto río Magdalena, (2) Cuencas del bajo río Magdalena ? Bajo río Cauca ? río San Jorge, y (3) Cuenca del río Sinú. Entre las UM se detectaron niveles bajos a moderados de flujo genético que es principalmente unidireccional de las UM 1 y 3 a la UM 2.

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Microsatellite DNA markers for Podocnemis unifilis, the endangered yellow‐spotted Amazon River turtle

Cited by 16 publications

References 8 publications

Polyandry in Podocnemis unifilis (Pleurodira; Podocnemididae), the vulnerable yellow-spotted Amazon River turtle

Polyandry in Podocnemis unifilis (Pleurodira; Podocnemididae), the vulnerable yellow-spotted Amazon River turtle

Short Communication Polyandry in the red-headed river turtle Podocnemis erythrocephala (Testudines, Podocnemididae) in the Brazilian Amazon

Extremely low genetic diversity and weak population differentiation in the endangered Colombian river turtle Podocnemis lewyana (Testudines: Podocnemididae)

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