Microsatellite DNA Marker Analysis Revealed Low Levels of Genetic Variability in the Wild and Captive Populations of Cirrhinus cirrhosus (Hamilton) (Cyprinidae: Cypriniformes)

Assessment of genetic structure of wild populations of mrigal carp,Cirrhinus cirrhosusby microsatellite DNA markers

“…Their reported average number of alleles was 2.0. The results of our study are consistent with the findings of Hasanat et al () in most aspects, except the detection of polymorphism for the same five loci (16 for the six loci) whereas they detected polymorphism in three loci, findings of monomorphic alleles at locus Bgon17 , and slightly higher average number of alleles (2.4–2.8). The number of alleles obtained for these loci in our study is closer to those in the study of Lal et al (), who obtained 2–4 alleles using five markers ( MFW1 , MFW2 , MFW17 , Bgon22 , Barb54 ) from five populations of C. cirrhosus in India.…”

“…The H o and H e values indicated the high genetic diversity in the Padma population which might be due to the enormous size of this river with an area of 120 km in length and 4–8 km in width that provides the opportunity to form a vast gene pool, combination of populations from different geological points, natural selection supporting heterozygosity, or subdivision accompanied by genetic drift (Begum et al, ; Toro & Maki‐Tanila, ). But interestingly, when we compare the results with Hasanat et al () we can see three H o values out of nine were higher than the H e . In the present study, H o and H e of three populations ranged from 0.37 to 0.40 and 0.39 to 0.45 which are lower than those values of 0.44 and 0.54, respectively, obtained from the same five loci of C. cirrhosus by Hasanat et al ().…”

“…But interestingly, when we compare the results with Hasanat et al () we can see three H o values out of nine were higher than the H e . In the present study, H o and H e of three populations ranged from 0.37 to 0.40 and 0.39 to 0.45 which are lower than those values of 0.44 and 0.54, respectively, obtained from the same five loci of C. cirrhosus by Hasanat et al (). This data indicated that the river originated wild populations of mrigal have lost genetic variability over the 5 years for which specific reasons are difficult to explain.…”

“…Data from stock structure assessments can be crucial for appropriate planning of carps breeding programmes aimed at the conservation and maintenance of natural genetic variety. Hasanat et al () analysed C. cirrhosus collected from same three rivers using five of the six microsatellite loci ( MFW1 , MFW2 , MFW17 , Bgon22 , Barb54 ) used in this study and obtained 10 alleles ranging in size from 97 to 225 bp. Their reported average number of alleles was 2.0.…”

“…Now‐a‐days, various types of DNA markers are available, but microsatellites seem to be especially powerful for quantifying genetic variations within and between populations (O'Connell & Wright, ). However, Hasanat, Mollah, and Alam () conducted genetic structure analysis of this species from same rivers but from different locations. Constant monitoring of genetic structure baseline values is crucial to forecast long‐term trends and mitigate problems before they cause irreversible damage to natural stocks.…”