Microsatellite data resolve phylogeographic patterns in European grayling, Thymallus thymallus, Salmonidae

Koskinen, Mikko; Nilsson, Jan; Веселов, А. Е.; Потуткин, А. Г.; Ranta, Esa; Primmer, Craig R.

doi:10.1038/sj.hdy.6800072

Cited by 90 publications

(116 citation statements)

References 35 publications

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“…On the other hand, intrapopulation variability was rather low, as observed previously in other grayling populations. This low variability has been ascribed to the life history characteristics of grayling (Koskinen et al, 2002b). The only exception to this general finding is the observation of high intrapopulation variability in grayling populations from Bavaria, probably as a consequence of stocking activity (Gross et al, 2001).…”

Section: Introgression In Adriatic Graylingmentioning

confidence: 74%

“…According to external appearance, it is a very heterogeneous species, exhibiting several geographically confined and phenotypically distinct populations (Surre et al, 1986;Broughton, 2000). The heterogeneity has been recently revealed also at the DNA level, represented by extremely high levels of intraspecific genetic divergence (F st up to 0.74; Po0.001), even across short geographical distances (Koskinen et al, 2002b).…”

Section: Introductionmentioning

confidence: 99%

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Genetic introgression between wild and stocked salmonids and the prospects for using molecular markers in population rehabilitation: the case of the Adriatic grayling (Thymallus thymallus L. 1785)

Sušnik

Berrebi

Dovč³

et al. 2004

Heredity

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In the north Adriatic basin, a morphologically and genetically distinct lineage of grayling is found, designated as the Adriatic grayling. In Slovenia, the Adriatic grayling is restricted to the Soča river system, where it is critically endangered. The most pertinent threat is stocking with nonnative, highly divergent Sava (Danubian) drainage stock, and this activity has been going on for more than four decades. The present study was designed to characterise the genetic structure of the Adriatic grayling in Slovenia, with particular emphasis on estimating the degree of introgression with nonindigenous stocked grayling. We analysed polymorphism at 154 microsatellite loci in samples representing grayling from the Adriatic and Danubian drainage stock. A relatively high number (12) of alleles, diagnostic for the Adriatic grayling, were identified. However, a correspondence analysis based on individual multilocus genotypes also revealed that there is no distinctive Adriatic group but rather a dispersed multitude of individuals that cannot be unambiguously distinguished from the more homogenous Danubian population. A Bayesian analysis of individual admixture coefficients confirmed this pattern and revealed extensive introgression between the Adriatic grayling and stocked grayling of Danubian origin. Average individual admixture coefficients showed that only between 50 and 60% of the original gene pools remained, and only few non-introgressed indigenous individuals could be identified. Microsatellite-based individual admixture analysis appear to be an important tool for identifying remaining non-introgressed indigenous individuals that could be used for restoring the original populations.

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Section: Introgression In Adriatic Graylingmentioning

confidence: 74%

Section: Introductionmentioning

confidence: 99%

Genetic introgression between wild and stocked salmonids and the prospects for using molecular markers in population rehabilitation: the case of the Adriatic grayling (Thymallus thymallus L. 1785)

Sušnik

Berrebi

Dovč³

et al. 2004

Heredity

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“…A pesar de que los microsatélites poseen altas tasas de mutación, las regiones flanqueantes están más conservadas, y por tanto se pueden utilizar para establecer hipótesis filogenéticas entre especies cercanas. De esta forma se han resuelto filogenias a nivel de familia, para los cícli-dos (Zardoya et al, 1996) y salmónidos (Koskinen et al, 2002), de subfamilia para las avispas Polistinae (Zhu et al, 2000); y a nivel específico para el sapo corredor Bufo calamita (Rowe et al, 1998) o el cangrejo de herradura Limulus polyphemus (Ortí et al, 1997), entre otros.…”

Section: Origen Y Evolución De Los Microsatélitesunclassified

Microsatélites: sus aplicaciones en la conservación de la biodiversidad

González¹

2003

GRAELLSIA

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RESUMENEn los últimos años, la utilización de microsatélites como marcador molecular en estudios de conservación de la biodiversidad ha aumentado considerablemente, debido a dos características principales: su elevada tasa de polimorfismo y la posibilidad de poder trabajar con reducidas cantidades de ADN, que se pueden obtener sin que resulte necesario el sacrificio de ejemplares (es decir, no invasivas). Son particularmente útiles para resolver diversas cuestiones relacionadas con la conservación de la diversidad genómica, como la detección de posibles cuellos de botella, medida del flujo génico e hibridación entre poblaciones, determinación de paternidades, asignación de individuos a su población de origen y determinación de la estructura poblacional, entre otras. En este trabajo se revisa la información existente sobre microsatélites (características, ventajas y limitaciones), y en particular cuándo son más adecuados para resolver determinados problemas planteados para un organismo. Así mismo, se presentan consideraciones futuras de estos y otros marcadores alternativos. Palabras clave: Microsatélites, biodiversidad, conservación, genética de poblaciones, revisión. ABSTRACT Microsatellites: Application to biodiversity conservationMicrosatellites have been increasingly used as molecular marker in biodiversity conservation studies in the last years, due to two main characteristics: high level of polymorphism, and the possibility of working with low quantities of DNA which can be obtained without the sacrificing of the specie to study (non invasive samples). Microsatellite markers appear particularly useful to deal with issues such as detecting bottleneck, measuring gene flow and hybridization between populations, determining paternity, assigning individuals to their population of origin and determining population structure. This article highlights information about microsatellites (characteristics, advantages and limitations) that can be used to determine if they are suitable markers to solve particular problems about an organism. It also outlines future considerations of these and other alternative markers.

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“…The European grayling is widely distributed in northern and continental Europe from the Ural mountains to the British Isles (Northcote 1995), and its phylogeography has been studied intensively analysing both mitochondrial data and microsatellites (Koskinen et al 2000(Koskinen et al , 2002Gum et al 2005). Koskinen et al (2000) found three main assemblages of mitochondrial haplotypes that diverged before the late Pleistocene, and it was concluded that Northern Europe was colonized from at least two ancient refugia.…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial haplotype diversity of Gyrodactylus thymalli (Platyhelminthes; Monogenea): extended geographic sampling in United Kingdom, Poland, and Norway reveals further lineages

Hansen

Bachmann

2007

Parasitol Res

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In recent years, the mitochondrial haplotype diversity of the monogenean ectoparasites Gyrodactylus salaris Malmberg, 1957 on Atlantic salmon and G. thymalli Zitnan, 1960 on grayling has been studied intensively to understand the taxonomy and phylogeography of the two species. According to these studies, neither species can be considered monophyletic, but unfortunately, the geographic sampling has mostly been restricted to Fennoscandia. Only few samples from continental Europe have been analysed, and samples from the United Kingdom have not been included at all. Gyrodactylosis is a notifiable disease in Europe and is in the UK considered the most important exotic disease threat to wild Atlantic salmon populations. In this study, we report six new mitochondrial haplotypes of G. thymalli from England, Poland, and Norway detected by sequencing 745 bp of the cytochrome oxidase I gene. The six new haplotypes add five new clades to a neighbor-joining dendrogram deduced on the basis of the currently known 44 mitochondrial haplotypes for G. thymalli and G. salaris. We conclude that G. thymalli established in the UK along with the immigration of grayling. There is currently no reason to suspect that this parasite is a threat to Atlantic salmon in the UK, although its infectivity to salmon stocks in the UK has not been tested.

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Microsatellite data resolve phylogeographic patterns in European grayling, Thymallus thymallus, Salmonidae

Cited by 90 publications

References 35 publications

Genetic introgression between wild and stocked salmonids and the prospects for using molecular markers in population rehabilitation: the case of the Adriatic grayling (Thymallus thymallus L. 1785)

Genetic introgression between wild and stocked salmonids and the prospects for using molecular markers in population rehabilitation: the case of the Adriatic grayling (Thymallus thymallus L. 1785)

Microsatélites: sus aplicaciones en la conservación de la biodiversidad

Mitochondrial haplotype diversity of Gyrodactylus thymalli (Platyhelminthes; Monogenea): extended geographic sampling in United Kingdom, Poland, and Norway reveals further lineages

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