Microsatellite-based Genotyping of Candida albicans Isolated from Patients with Superficial Candidiasis

Abstract

This study aimed to examine the genotype distribution of Candida albicans and the major genotypes involved in superficial candidiasis. The genotypes of C. albicans isolated from the infection sites of patients with superficial candidiasis (referred to as infection isolates) were analyzed by fragment analysis using 4 microsatellite markers (HIS3, CDC3, CAI and CAIII). Genotypes of the infection isolates were compared with those of C. albicans isolated from oral mucosa of non-candidiasis patients (referred to as oral isolates). Isolates of C. albicans showed 4 major genotypes for HIS3/CAI (" a " for 148 : 148 / 23 : 23," b " for 148 : 160 / 33 : 41," c " for 148 : 164 / 32 : 41 and " d " for 152 : 152 / 18 : 27). The genotypes " a "," b " and " d " were commonly found in oral (4.7, 8.8 and 7.6%, respectively) and infection (6.6, 9.2 and 15.4%, respectively) isolates. No isolates of genotype " c " were isolated from infection sites. The genotype " a " was found in the isolates from patients with genitalia candidiasis. Genotyping of multiple isolates from an individual patient showed that C. albicans from infection sites was genetically homogenous as compared with that of oral isolates, even in the same patient with candidiasis.

OralCandida albicanscolonization in healthy individuals: prevalence, genotypic diversity, stability along time and transmissibility

“…In hospitalized patients without oral infections, up to nine different C. albicans genotypes in the same individual were identified by using two molecular markers (Hist3 and CAI) [42]. Analysing only the CAI marker in the profile of isolates from [42] study, 74% of those patients (n = 17) had only one CAI genotype in the different clones, while 22% of the patients presented two CAI genotypes (n = 5). In the five cases with two CAI genotypes, only one could be explained by LOH, a simple mutational step, while the other four cases consisted of different CAI genotypes.…”

Genotypic diversity and antifungal susceptibility pattern of Candida albicans species isolated from hospitalized paediatric patients with urinary tract infection in Iran

“…Among the different techniques described for Candida albicans typing are multilocus enzyme electrophoresis, random amplification of polymorphic DNA, amplified fragment length polymorphism, repetitive element sequence‐based PCR, multilocus sequence typing, internal transcribed spacer sequencing and microsatellite length polymorphism (MLP) (Shimizu et al . 2011). MLP typing is based on short tandem repeats (STRs) that are highly variable and polymorphic (Sampaio et al .…”

Molecular Epidemiology of Japanese Isolates of <i>Microsporum canis</i> Based on Multilocus Microsatellite Typing Fragment Analysis

“…MS fragment analysis has recently been utilized in molecular epidemiology, with MS analysis of several different loci, called multilocus microsatellite typing (MLMT), possibly being the most sensitive method currently available for detection of intraspecies polymorphisms (10). MLMT has been used to genotype Trichophyton rubrum (11) and Candida albicans (12), and for the analysis of M. canis strains isolated from individuals with zoonosis (13,14). This study utilized MLMT to detect intraspecies polymorphisms of M. canis strains isolated from both humans and domesticated cats in Japan.…”