Microsatellite analysis of Puccinia triticina from Triticum and Aegilops hosts

Gultyaeva, E. I.; Shaydayuk, E. L.; Казарцев, И. А.; Akhmetova, Aigul; Kosman, Evsey

doi:10.1007/s13313-018-0542-3

Cited by 7 publications

(5 citation statements)

References 19 publications

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“…Currently, studies of genetic diversity in populations of P . triticina conducted by other researchers are based on isolates collected from common wheat, durum wheat, and wild wheat species (Goyeau et al, 2007; Gultyaeva et al, 2016, 2018, 2020, 2021; Kolmer et al, 2013, 2015; Kosman et al, 2014). Thus, no information is available regarding the variability and structure in European populations of P .…”

Section: Discussionmentioning

confidence: 99%

Pathogenic and genetic diversity ofPuccinia triticinafrom triticale in Poland between 2012 and 2015

et al. 2021

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Triticale (×Triticosecale), the first human-made crop species in a hexaploid form (AABBRR), was obtained by crossing tetraploid wheat (Triticum durum or T. turgidum) with cereal rye (Secale cereale or S. montanum) (Ammar et al., 2004). The first commercially available triticale cultivars for farmers became available in the 1960s.Recently, the global cultivated area of triticale has been estimated at approximately 4 million hectares, with the largest areas occurring in Poland, Belarus, Germany, and France. In Poland, triticale is grown on approximately 1.3 million hectares. In general, triticale combines the high grain quality and yield potential of wheat with the resistance to biotic and abiotic stresses of rye (Ammar et al., 2004; Audenaert et al., 2014).Leaf (brown) rust, which is caused by Puccinia triticina, is an important wheat disease in all geographic regions of the world where wheat is cultivated (Bolton et al., 2008). The development of leaf rust is strongly influenced by environmental conditions, among which temperature and moisture play the most significant role (Kaul & Shaner, 1989). In Poland, under favourable disease conditions, a peak of leaf rust development may start in June and last until leaves senesce at the end of July. For many years, triticale was considered

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Section: Discussionmentioning

confidence: 99%

Pathogenic and genetic diversity ofPuccinia triticinafrom triticale in Poland between 2012 and 2015

et al. 2021

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“…The isolates collected from the affected plants of Northern Kazakhstan are similar in virulence to the population of Western Siberia [63]. Extensive studies of the population structure revealed that all pathotypes isolated in the regions of Western Siberia, the Urals, and Northern Kazakhstan were avirulent to the Lr19 and Lr24 genes [64,65]. Earlier, it was also reported about the avirulence of the Kazakhstan South-Eastern population to the genes Lr9, Lr19, Lr24, Lr25, and Lr28, and that of the North Kazakhstani population to Lr19, Lr24, Lr25, Lr28, Lr36 and Lr45 [50].…”

Section: Discussionmentioning

confidence: 92%

Phenotyping and Identification of Molecular Markers Associated with Leaf Rust Resistance in the Wheat Germplasm from Kazakhstan, CIMMYT and ICARDA

Malysheva,

Kokhmetova,

Urazaliev

et al. 2023

Plants

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Leaf rust (LR) is the most widespread disease of common wheat worldwide. In order to evaluate leaf rust resistance, 70 uncharacterized wheat cultivars and promising lines with unknown leaf rust resistance genes (Lr genes) were exposed to Kazakhstani Puccinia triticina (Pt) races at the seedling stage. Field tests were performed to characterize leaf rust responses at the adult plant growth stage in the 2020–2021 and 2021–2022 cropping seasons. The wheat collection showed phenotypic diversity when tested with two virulent races of Pt. Thirteen wheat genotypes (18.6%) showed high resistance at both seedling and adult plant stages. In most cases, breeding material originating from international nurseries showed higher resistance to LR. Nine Lr genes, viz. Lr9, Lr10, Lr19, Lr26, Lr28, Lr34, Lr37, Lr46, and Lr68, either singly or in combination, were identified in 47 genotypes. Known Lr genes were not detected in the remaining 23 genotypes. The most commonly identified resistance genes were Lr37 (17 cultivars), Lr34 (16 cultivars), and Lr46 (10 cultivars), while Lr19, Lr68, Lr26, and Lr28 were the least frequent. Four Lr genes were identified in Keremet and Hisorok, followed by three Lr genes in Aliya, Rasad, Reke, Mataj, Egana and Almaly/Obri. The molecular screening revealed twenty-nine carriers of a single Lr gene, ten carriers of two genes, six carriers of three genes, and two carriers of four genes. Most of these accessions showed a high and moderate level of APR (Adult plant resistance) and may be utilized for the incorporation of Lr genes in well-adapted wheat cultivars. The most effective combination was Lr37, Lr34, and Lr68, the carriers of which were characterized by a low disease susceptibility index. The obtained results will facilitate breeding programs for wheat resistance in Kazakhstan.

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“…Many studies on genetic diversity have reported the significant correlation between the virulence phenotypes and molecular genotypes of P. triticina in Pakistan, Europe, Central Asia, and the Caucasus regions (Halkett et al, 2005;Kolmer and Ordoñez, 2007;Mantovani et al, 2010;Kolmer et al, 2012;Kolmer et al, 2017). However, Park et al (2000); Prasad et al (2017), and Gultyaeva et al (2018) thought that there was no significant correlation between virulence polymorphism and molecular polymorphism of the P. triticina population diversity in Western Europe, South Asia, or Russia and Kazakhstan. There was also no significant correlation between virulence polymorphism and molecular polymorphism of the P. triticina population in China (Xu et al, 2002;Pu et al, 2004;Xu et al, 2013;Zhao, 2013), which was consistent with the results of this study.…”

Section: Discussionmentioning

confidence: 99%

“…(2000) ; Prasad et al. (2017) , and Gultyaeva et al. (2018) thought that there was no significant correlation between virulence polymorphism and molecular polymorphism of the P. triticina population diversity in Western Europe, South Asia, or Russia and Kazakhstan.…”

Section: Discussionmentioning

confidence: 99%

Virulence and molecular genetic diversity, variation, and evolution of the Puccinia triticina population in Hebei Province of China from 2001 to 2010

Zhang

Zhang²,

Meng³

et al. 2023

Front. Plant Sci.

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Wheat leaf rust, caused by Puccinia triticina, is one of the most important fungal diseases of wheat in China. However, little is known about the dynamic changes of population structure and genetic diversity of P. triticina during a period of time. In this study, 247 isolates of P. triticina collected from Hebei Province from 2001 to 2010 were tested on 36 Thatcher near-isogenic lines for virulence diversity and detected by 21 pairs of Expressed Sequence Tag derived Simple Sequence Repeat (EST-SSR) primers for genetic diversity. A total of 204 isolates were successfully identified as 164 races, and THTT, THST, PHRT, THTS, and PHTT were the most common races in Hebei Province from 2001 to 2010. The cluster analysis based on virulence showed that P. triticina has a rich virulence polymorphism, which had a certain correlation with the years, while the cluster analysis based on EST-SSR showed that the genetic diversity of the P. triticina population was significantly different between years in Hebei Province from 2001 to 2010. In addition, the population structure of P. triticina may have changed greatly in 2007 and 2009, which was significantly different from that of 2001–2006 on either virulence or genetic characteristics. The variation frequency of the population structure had an increasing trend during this period. From 2001 to 2010, there was a certain degree of gene flow among the P. triticina populations. No significant correlation was found between virulence and molecular polymorphism. The genetic differentiation analysis of the 10 tested populations (each year as a population) showed that the coefficient of genetic differentiation (Gst) was 0.27, indicating that there was a certain genetic differentiation among or within populations of P. triticina in Hebei Province. The genetic variation within populations (73.08%) was higher than that among populations (26.92%), which indicated that the genetic variations were mainly found within populations. Our study provides the foundation for a better understanding of the population structure change and genetic diversity of P. triticina over a period in Hebei Province of China.

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Microsatellite analysis of Puccinia triticina from Triticum and Aegilops hosts

Cited by 7 publications

References 19 publications

Pathogenic and genetic diversity ofPuccinia triticinafrom triticale in Poland between 2012 and 2015

Pathogenic and genetic diversity ofPuccinia triticinafrom triticale in Poland between 2012 and 2015

Phenotyping and Identification of Molecular Markers Associated with Leaf Rust Resistance in the Wheat Germplasm from Kazakhstan, CIMMYT and ICARDA

Virulence and molecular genetic diversity, variation, and evolution of the Puccinia triticina population in Hebei Province of China from 2001 to 2010

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