Microsatellite Analysis of Genetic Diversity in Pekin(Anas platyrhynchos) and Muscovy (Cairina moschata)Duck Populations

Ahmadi, Alireza; Rahimi, G.; Vafaei, Afshin; Sayyazadeh, H.

doi:10.3923/ijps.2007.378.382

Cited by 20 publications

(5 citation statements)

References 13 publications

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“…Among different loci analysed, the locus CAUD024 had the highest observed (0.8351) and expected (0.9211) heterozygosity in most of the duck populations (Table 2). Similar heterozygosity value of less than 0.6 was observed by Paulus and Tiederman (2003), Ahmadi et al (2007), Gaur Generally, a marker to be considered useful for measuring genetic variation in a population should have a heterozygosity value of 0.3 to 0.8. Hence, the markers used in this study are quite suitable for assessing the genetic diversity in duck populations as the range of heterozygosity found in this study fit well within the specified range.…”

Section: Heterozygositymentioning

confidence: 57%

“…Results revealed that the highest mean observed number of alleles ( Ahmadi et al (2007). Among Indian duck populations, less number of observed alleles was reported in Moti (3.4) and Indian Runner (3.3) by Kumar et al (2011), while higher observed number of alleles (8.0) was reported in Assam ducks by Mukesh et al (2011).…”

Section: Microsatellite Allelic Diversitymentioning

confidence: 82%

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Genetic Diversity of Six Duck Populations in India

Veeramani

Prabakaran

Sivaselvam

et al. 2021

IJAR

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Background: The variety of indigenous duck germplasm contribute maximum to the poultry industry in India, besides chicken population. In southern part of India, the available duck genetic resource, particularly Arni ducks (comprises of Sanyasi and Keeri ducks) of Tamil Nadu, has its own characteristics with innate potentiality of higher productivity without any input system of management. Genetic characterization and diversity of indigenous duck genetic resources has not been properly studied. In the present study, the genetic diversity of Arni ducks with other indigenous and exotic duck germplasm were analysed with microsatellite markers. Method: Genomic DNA was isolated from the blood samples of six duck populations. Molecular characterization was carried out with duck specific FAO recommended microsatellite markers. The genotyping of ducks was done based on the size of 4324 PCR amplicons of 23 microsatellite loci, which were subjected to capillary electrophoresis using automatic sequencer.Result: A total of 222 alleles in six duck populations across 23 microsatellite loci with a mean of 9.65 ± 0.95 alleles were found. Kuttanad duck variety had the highest number of alleles (139) followed by Sanyasi (136), Keeri (129), Muscovy (118), Assam (91) and White Pekin (78) ducks. The mean observed number of alleles was 6.04 ± 0.59, 5.91 ± 0.76, 5.61 ± 3.17, 5.13 ± 0.44, 3.96 ± 0.76 and 3.39 ± 0.40 in Kuttanad, Sanyasi, Keeri, Muscovy, Assam and White Pekin ducks respectively. The overall mean polymorphism information content (PIC) values among the six duck populations was 0.6269. In most of the duck populations, the mean PIC value was more than 0.5 except in Assam (0.4815) and White Pekin (0.3725) ducks. The observed heterozygosity was the highest in Keeri ducks (0.5217) and lowest in White Pekin ducks (0.2766), while, the mean expected heterozygosity was the highest in Sanyasi (0.5628) and lowest in White Pekin (0.4038) ducks. The variations in the observed and expected number of alleles, differences in PIC of various microsatellite loci might be attributed to the genetic variability of the duck populations, number and type of microsatellite primers utilised for analysis and the genetic diversity of the duck breeds under study. Higher FST value indicates the substantial degree of breed differentiation among the studied duck populations.

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Section: Heterozygositymentioning

confidence: 57%

Section: Microsatellite Allelic Diversitymentioning

confidence: 82%

Genetic Diversity of Six Duck Populations in India

Veeramani

Prabakaran

Sivaselvam

et al. 2021

IJAR

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“…Carco et al (2018) showed the average observed heterozygosity for the Polish population, 0.692 and 0.652, respectively, while the Italian breeds had lower values: 0.450 for AGV and 0.372 for AMG. Ahmadi et al (2007) also found similar levels of genetic variation within the Peking and Muscovy populations with values of heterozygosity of 0.530 and 0.440, respectively.…”

Section: Genetic Diversity Within the Breedsmentioning

confidence: 53%

Microsatellites and Morphometric Diversity Among Indonesian Local Duck Populations in Lombok Island

Maskur¹,

Muhsinin²,

Lestari³

et al. 2021

AAVS

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The genetic diversity of three breeds of Indonesian local duck in Lombok island West Nusa Tenggara province locus was investigated based on morphometric traits and four microsatellites marker. Experimental ducks consisted of 75 Sasak ducks, 75 Khaki Campbell ducks and 70 Mojosari ducks. Morphometric traits were recorded and statistically analyzed, whereas genetic parameters of microsatellite loci were calculated using PopGene32. In this study, the mean PIC of the 4 microsatellite loci in all populations was 0.472, whereas AY287 was the most polymorphic locus with a PIC value of 0.735 and APL579 was a monomorphic locus with a PIC value of 0 (zero). The mean value of observed heterozygosity (Ho) is lower than expected (He), with the values 0.393 and 0.472 (Khaki Campbell); 0.433 and 0.532 (Mojosari); and 0.330 and 0.451 (Sasak), respectively. This indicates that the population is heading towards a heterozygosity deficit and proves that the three populations are not in Hardy-Weinberg equilibrium. The inbreeding coefficient in the three populations was quite high with a positive Fis value ranging from 0.000 -0.422. Meanwhile, the value of the genetic differentiation index (Fst) in all populations is zero (0), indicating that there is no differentiation between breeds at the observed loci.

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“…Маркери SMO7, SMO8 і SMO13 були мономорфними для обох популяцій. Показник загальної гетерозиготності для пекінської породи качок становив 0,53, а для мускусної 0,44 (Khan Ahmadi et al, 2007).…”

Section: результати та їх обговоренняunclassified

Shaoxing breed duck polymorphism by microsatellite loci

Chepiha¹,

Kostenko²,

Doroshenko³

et al. 2018

SMLNUVMBA

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Microsatellite markers are now been widely used for the detection and description of micropopulation processes occurring in the populations of domestic animals for the effects of various factors of breeding pressure. Microsatellite loci distributed throughout eukaryotic genomes, making them the preferred genetic marker for high resolution genetic mapping. In recent years, rapid advances have been made in the development of molecular genetic maps. High-density linkage maps are now available for many farm animals, such as cattle, pigs, and goats. In contrast, mapping studies in avian species are much less advanced except in the chicken. According to FAO about 70% of ducks are bred in China. This country is a leader in growing ducks. The Shaoxing breed is one of the three major duck breeds in China. Ducks of this breed are characterized by high performance. According to the Bureau of Product Quality, the age of maturity (the beginning of egg laying) in these birds occurs at 130-140 days. The characteristics of the Shaoxing breed include the fact that the peak period of laying eggs lasts from eight to ten months. On average, one duck in 500 days gives from 290 to 310 eggs, which is one of the highest rates for egg breeds. That is why the purpose of our study was the microsatellite analysis of two populations of Shaoxing breed with 9 locuses was conducted. The selection of birds for the study were carried out on a duck farms in Zhejiang Generation Biological Science and Technology Co., Ltd. and Zhuji Guowei Poultry Development Co, Ltd., and at the laboratory of the Jjejiang Academy of Sciences Institute. Samples collection and DNA preparation: Venous blood samples were collected from 480 ducks (240 ducks of population I and 240 ducks of population II of the Shaoxing breeds) of both populations into 3 ml tubes containing EDTA as anticoagulant agent. In total of 9 investigated loci in the Shaoxing breed population, only one locus was monomorphic (SMO10). The number of different alleles (Na) for each polymorphic locus ranged from 2 (SMO12) to 13 (APL79, CMO11) in population I and from 2 (APL78, SMO12) to 7 (APL79) in population II. On average, one locus had 5.889 alleles in population I and 3.889 of alleles in the population II. The effective number of alleles (Nе) was 1.735 in population I and 1.599 in population II. The number of alleles and the expected heterozygosity (Hexp) values can provide important information for the discrimination of individuals and breeds. The index of expected heterozygosity in population I was 0.336 and 0.307 in population II. The information index (I) was 0,702 in population I and 0,576 in population II. For each population was found private alleles, in population I 6 alleles and in population II just 4 alleles. The results show high level of polymorphism of the studied populations of ducks. The obtained results can be used in the creation of new lines of ducks.

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Microsatellite Analysis of Genetic Diversity in Pekin(Anas platyrhynchos) and Muscovy (Cairina moschata)Duck Populations

Cited by 20 publications

References 13 publications

Genetic Diversity of Six Duck Populations in India

Genetic Diversity of Six Duck Populations in India

Microsatellites and Morphometric Diversity Among Indonesian Local Duck Populations in Lombok Island

Shaoxing breed duck polymorphism by microsatellite loci

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