Microsatellite Analysis of Genetic Diversity and Population Structure of the Iranian Kurdish Horse

Amjadi, Motahareh; Yeganeh, Hassan Mehrbani; Sadeghi, Mostafa; Raza, Sayed Haidar Abbas; Yang, Jinmeng; Najafabadi, Hamed Amirpour; Batool, Uzma; Shoorei, Hamed; Abdelnour, Sameh A.; Ahmed, Jam Zaheer

doi:10.1016/j.jevs.2020.103358

Cited by 6 publications

(5 citation statements)

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“…The values of Na, Ho, and F represent the degree of population genetic differentiation, with higher values of Na and Ho and negative values of F indicating greater genetic diversity [ 28 – 30 ]. When He exceeds Ho, it suggests a lack of heterozygosity within the population [ 31 , 32 ]. Additionally, the polymorphic information content (PIC) was calculated, with higher PIC values indicating greater genetic variation.…”

Section: Methodsmentioning

confidence: 99%

The genetic diversity of Oncomelania hupensis robertsoni, intermediate hosts of Schistosoma japonicum in hilly regions of China, using microsatellite markers

Song,

Wang,

et al. 2024

Parasites Vectors

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Abstract Background The elimination of schistosomiasis remains a challenging task, with current measures primarily focused on the monitoring and control of Oncomelania hupensis (O. hupensis) snail, the sole intermediate host of Schistosome japonicum. Given the emerging, re-emerging, and persistent habitats of snails, understanding their genetic diversity might be essential for their successful monitoring and control. The aims of this study were to analyze the genetic diversity of Oncomelania hupensis robertsoni (O. h. robertsoni) using microsatellite DNA markers; and validate the applicability of previously identified microsatellite loci for O. hupensis in hilly regions. Methods A total of 17 populations of O. h. robertsoni from Yunnan Province in China were selected for analysis of genetic diversity using six microsatellite DNA polymorphic loci (P82, P84, T4-22, T5-11, T5-13, and T6-27). Results The number of alleles among populations ranged from 0 to 19, with an average of 5. The average ranges of expected (He) and observed (Ho) heterozygosity within populations were 0.506 to 0.761 and 0.443 to 0.792, respectively. The average fixation index within the population ranged from – 0.801 to 0.211. The average polymorphic information content (PIC) within the population ranged from 0.411 to 0.757, appearing to be polymorphic for all loci (all PIC > 0.5), except for P28 and P48. A total of 68 loci showed significant deviations from Hardy-Weinberg equilibrium (P < 0.05), and pairwise Fst values ranged from 0.051 to 0.379. The analysis of molecular variance indicated that 88% of the variation occurred within snail populations, whereas 12% occurred among snail populations. Phylogenetic trees and principal coordinate analysis revealed two distinct clusters within the snail population, corresponding to “Yunnan North” and “Yunnan South”. Conclusions O. h. robertsoni exhibited a relatively high level of genetic differentiation, with variation chiefly existing within snail populations. All snail in this region could be separated into two clusters. The microsatellite loci P82 and P84 might not be suitable for classification studies of O. hupensis in hilly regions. These findings provided important information for the monitoring and control of snail, and for further genetic diversity studies on snail populations. Graphical Abstract

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Section: Methodsmentioning

confidence: 99%

The genetic diversity of Oncomelania hupensis robertsoni, intermediate hosts of Schistosoma japonicum in hilly regions of China, using microsatellite markers

Song,

Wang,

et al. 2024

Parasites Vectors

View full text Add to dashboard Cite

show abstract

“…The values of Na, Ho, and F measure the degree of population genetic differentiation, with higher value of Na and Ho and negative values of F, indicating greater genetic diversity [28][29][30] . When He exceeds Ho, it suggests a lack of heterozygosity within population [31,32] . Additionally, the polymorphic information content (PIC) was calculated, with higher PIC values indicating greater genetic variation.…”

Section: Analysis Of Microsatellite Diversitymentioning

confidence: 99%

The genetic diversity of Oncomelania hupensis robertsoni, intermediate hosts Schistosome japonicum in hilly regions of China using microsatellite markers

Song,

Wang,

et al. 2024

Preprint

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Background The elimination of schistosomiasis remains a challenging task, with current measures primarily focused on the monitoring and control of Oncomelania hupensis (O. hupensis) snail, the sole intermediate host Schistosome japonicum. Given the emerging, re-emerging, and persistent habitats of snails, a study on the genetic diversity of snails might be an essential. Objective The aims of this study were to (1) analyze the genetic diversity of O. hupensis robertsoni Yunnan strain (O. h. r. Yunnan strain) using microsatellite DNA markers; and (2) validate the applicability of previously identified microsatellite loci for O. hupensis in hilly regions. Methods A total of 17 populations of O. hupensis from Yunnan Province in China were selected for analysis of genetic diversity by 6 microsatellite DNA polymorphic loci (i.e., P82, P84, T4-22, T5-11, T5-13, T6-27). Results the number of alleles among populations ranged from 0 to 19, with an average of 5. The average ranges of expected (He) and observed (Ho) heterozygosity within populations were 0.506 to 0.761 and 0.443 to 0.792, respectively. The average fixation index within population ranged from − 0.801 to 0.211. The average polymorphic information content (PIC) within population ranged from 0.411 to 0.757, appearing polymorphic for all loci (all PIC > 0.5), except for P28 and P48. A total of 68 loci showed significant deviations from Hardy-Weinberg equilibrium (P < 0.05), and pairwise Fst values ranged from 0.051 to 0.379. The analysis of molecular variance indicated that 88% of the variation exists within snail populations, while 12% exists among snail populations. Phylogenetic trees and principal coordinate analysis revealed two distinct clusters within the snail population, corresponding to "Yunnan North" and "Yunnan South". Conclusion O. h. r. Yunnan strain exhibits a relatively high level of genetic differentiation, with variation chiefly existing within snail populations. All snail could be separated into two clusters. The microsatellite loci P82 and P84 may not be suitable for the classification study of O. hupensis in hilly regions. These findings provide the important information for monitoring and control of snail, and further genetic diversity studies on snail populations.

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“…The prominent forehead, muscular body, short and strong forelimbs and hindlimbs are the specific morphological indicators of this breed. According to the last official statistics published, the total number of horses in the country is approximately 155,000, of which the Kurdish horse has a population of approximately 2700 (Ala‐Amjadi et al., 2017). Both natural breeding and artificial insemination with frozen–thawed semen are used by breeders and owners.…”

Section: Introductionmentioning

confidence: 99%

Morphology and morphometry of sperm in Kurdish stallions, a local breed from western Iran

Nooroozian,

Goodarzi,

Tafti

2024

Reprod Domestic Animals

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The present work was designed for a thorough investigation into the sperm morphology and morphometry of Kurdish stallions. The semen samples were collected from 10 Kurdish stallions. Three preparations from each ejaculate were stained with eosin–nigrosin (EN), Diff‐Quik (DQ) and Rose Bengal (RB). The area, perimeter, length and width of the sperm head as well as tail length and total sperm length were measured. The parameters ellipticity, elongation, roughness and regularity were calculated. The morphology of sperm was also investigated under scanning and transmission electron microscopes. DQ and RB provided more clarified images for examining sperm structures compared to the EN method. The head length, head width, area and perimeter in EN were significantly higher than those in DQ and RB (p ≤ .05). Furthermore, the difference in head width, head area and head perimeter between DQ and RB was not significant (p ≥ .05). The tail length and total sperm length in all methods were close together (p ≥ .05). The highest percentage of normal sperm was seen in DQ and RB methods (82.55 ± 2.88 and 88.31 ± 5.19) respectively. The highest values for ellipticity, elongation and regularity were found in RB, whereas the highest value for roughness was measured in EN. Tail defects including coiled tails, and folded midpieces were the most frequent. Scanning electron microscope revealed two types of head shapes: heads with round anterior border, and heads with flat anterior border. The results indicated that despite the routine use of EN for morphological assessment of stallion sperm, RB and DQ can be considered for more clarified details of sperm structure including acrosome and midpiece. Furthermore, the Kurdish stallion sperm has morphometric traits in the normal range established for stallions; yet, some traits were larger than those reported for other breeds. It seems that the sperm of the Kurdish stallion has a longer head and tail in comparison with other horse breeds.

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Microsatellite Analysis of Genetic Diversity and Population Structure of the Iranian Kurdish Horse

Cited by 6 publications

References 5 publications

The genetic diversity of Oncomelania hupensis robertsoni, intermediate hosts of Schistosoma japonicum in hilly regions of China, using microsatellite markers

The genetic diversity of Oncomelania hupensis robertsoni, intermediate hosts of Schistosoma japonicum in hilly regions of China, using microsatellite markers

The genetic diversity of Oncomelania hupensis robertsoni, intermediate hosts Schistosome japonicum in hilly regions of China using microsatellite markers

Morphology and morphometry of sperm in Kurdish stallions, a local breed from western Iran

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