Microsatellite analysis of Fasciola spp. in Egypt

Abstract

Recently, the topic of diversity in Fasciola population in Egypt is controversial. The present study was performed to study the genetic diversity of isolated flukes based on microsatellites markers. Fasciola worms were collected from different hosts and geographical locations in Egypt. Control samples of Fasciola hepatica from France as well as Fasciola gigantica from Cameroon were included in the study. Collected flukes were identified morphologically and subjected for analysis using four microsatellite markers. Results of microsatellite profile (FM1 and FM2) proved that both species of Fasciola are distributed in Egypt irrespective of geographical location and host. Nevertheless, the microsatellite profile of some analyzed loci (FM2 and FM3) proved that Egyptian flukes showed more alleles compared to the reference ones. Differences of microsatellite profile in Egyptian isolates than that of corresponding reference samples indicate the remarkable diversity of these isolates. The present results highlighted the utility of microsatellite profile to discriminate between Fasciola species and to elucidate the diversity within the species. To our knowledge, this is the first time to study microsatellite polymorphism in Fasciola populations in Egypt.

Characterisation of a novel panel of polymorphic microsatellite loci for the liver fluke, Fasciola hepatica, using a next generation sequencing approach

“…In particular one study showed that the F . hepatica loci identified by Hurtrez-Boussès et al (2004) could be used for the related species Fasciola gigantica ( Dar et al, 2011 ), indicating the potential of the microsatellite panel described within this study to be used for other Fasciola species. Nevertheless, with the expansion of next generation sequencing technologies, large genomic datasets are more readily available for mining, particularly for genetic markers, providing potential for a F .…”

“…Studies have shown that microsatellites developed for one species can often be used in related species ( Jarne and Lagoda, 1996; Gemmell et al, 1997; Dar et al, 2011 ). In particular one study showed that the F .…”

“…hepatica populations involve the application of mitochondrial markers and/or small panels of microsatellites (reviewed by Hodgkinson et al, 2013 ). To date only five polymorphic microsatellites have been developed and applied to Fasciola populations in Spain and Eqypt ( Hurtrez-Boussès et al, 2004; Dar et al, 2011, 2013; Vilas et al, 2012 ). With the advancement of next generation technologies, trematode genome datasets are now being mined for microsatellite markers to develop panels of polymorphic markers; for example Schistosoma japonicum ( Xiao et al, 2011 ) and more recently Fascioloides magna ( Minarik et al, 2014 ).…”

Dinámica de la infestación por Fasciola hepatica en el sur de la provincia de Entre Ríos

“…Es entonces necesario, en algunos casos, el empleo de marcadores de mayor polimorfismo. En este sentido, una opción son los marcadores microsatélites o secuencias repetidas en tándem (SRT) (Hurtrez-Boussès et al, 2004;Dar et al, 2011Dar et al, , 2013Vilas et al, 2012;Cwiklinsky et al, 2015,). Los SRT son secuencias cortas repetidas de bases, situadas al azar en el genoma eucariota (Toth et al, 2000).…”

“…Los SRT son secuencias cortas repetidas de bases, situadas al azar en el genoma eucariota (Toth et al, 2000). Por su mayor polimorfismo son valorados para evaluar la diversidad genética y la estructura de poblaciones y subpoblaciones (Dar et al, 2011;Hurtrez-Bousses et al, 2004). Un…”