Microsatellite analysis of Aegilops tauschii germplasm

Pestsova, Elena; Korzun, Viktor; Гончаров, Н. П.; Hammer, Karl; Ganal, Martin W.; Röder, Marion S.

doi:10.1007/s001220051456

Cited by 100 publications

(78 citation statements)

References 15 publications

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“…Comparing the number of alleles detected in the accessions of common wheat in our study of T. dicoccoides (Fahima et al 2002) and of Ae. tauschii (Pestsova et al 2000) for the commonly used microsatellite markers (Table 7), it is evident that almost the same allele distribution and number for single loci was found in the accessions of common wheat, of wild emmer wheat and of Ae. tauschii, respectively, though the number of investigated accessions was different.…”

Section: Discussionmentioning

confidence: 99%

“…Some accessions possessing rare alleles for many different loci could be used for broadening the allelic diversity and the potential for cultivar improvement of wheat. Fahima et al (2002) and Pestsova et al (2000), respectively…”

Section: Discussionmentioning

confidence: 99%

“…Ganal tifying QTLs (Parker et al 1998), marker-assisted selection in wheat (Korzun et al 1998;Huang et al 2000a) and verifying the integrity and genetic stability of gene bank accessions (Börner et al 2000a). Such markers also revealed a high level of polymorphism among diploid species , in the accessions of tetraploid wild wheat Triticum dicoccoides (Fahima et al 2002) and of Aegilops tauschii, the D-genome donor of bread wheat (Pestsova et al 2000), and as well as in hexaploid wheat varieties (Plaschke et al 1995;Donini et al 1998;Prasad et al 2000;Stachel et al 2000).…”

Section: Introductionmentioning

confidence: 99%

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Assessing genetic diversity of wheat (Triticum aestivum L.) germplasm using microsatellite markers

et al. 2002

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A set of 24 wheat microsatellite markers, representing at least one marker from each chromosome, was used for the assessment of genetic diversity in 998 accessions of hexaploid bread wheat ( Triticum aestivum L.) which originated from 68 countries of five continents. A total of 470 alleles were detected with an average allele number of 18.1 per locus. The highest number of alleles per locus was detected in the B genome with 19.9, compared to 17.4 and 16.5 for genomes A and D, respectively. The lowest allele number per locus among the seven homoeologous groups was observed in group 4. Greater genetic variation exists in the non-centromeric regions than in the centromeric regions of chromosomes. Allele numbers increased with the repeat number of the microsatellites used and their relative distance from the centromere, and was not dependent on the motif of microsatellites. Gene diversity was correlated with the number of alleles. Gene diversity according to Nei for the 26 microsatellite loci varied from 0.43 to 0.94 with an average of 0.77, and was 0.78, 0.81 and 0.73 for three genomes A, B and D, respectively. Alleles for each locus were present in regular two or three base-pair steps, indicating that the genetic variation during the wheat evolution occurred step by step in a continuous manner. In most cases, allele frequencies showed a normal distribution. Comparative analysis of microsatellite diversity among the eight geographical regions revealed that the accessions from the Near East and the Middle East exhibited more genetic diversity than those from the other regions. Greater diversity was found in Southeast Europe than in North and Southwest Europe. The present study also indicates that microsatellite markers permit the fast and high throughput fingerprinting of large numbers of accessions from a germplasm collection in order to assess genetic diversity.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Assessing genetic diversity of wheat (Triticum aestivum L.) germplasm using microsatellite markers

et al. 2002

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“…Based on SSR analysis, the genetic polymorphism among species has been extensively investigated, and the cytological markers, including the chromosome-, genomic-or species specific markers, were also effectively developed in plants (Cuadrado & Schwarzacher 1998;Fuchs et al 1998;Schmidt & Heslop-Harrison, 1996), while chromosome identification and genome organization are also observed by SSR analysis such as Aegilops tauschii (Pestsova 2000), Lophopyrum elongatum (Mullan et al 2005) and Dasypyrum ). In our previous study, 102 SSR primer pairs distributing in all chromosomes of T. aestivum, were investigated on Secale, wheat -Secale amphiploid and its derivatives using Chinese Spring wheat as control.…”

Section: Discussionmentioning

confidence: 99%

Isolation and chromosomal localization of new MITE-like sequences from Secale

Zeng

Liu

et al. 2012

Biologia

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A species-specific DNA sequence (marker) that can detect the presence of Secale cereale chromatin in common wheat background was developed by using wheat microsatellite primer Xgwm614. One rye-specific DNA amplified fragment of 416bp (pSa614416) was obtained from Secale africanum and a wheat -S. africanum amphiploid. The primer Xgwm614 also gave rise to specific bands in all Chinese Spring-Imperial rye addition lines 1R to 7R. Sequence analysis revealed that pSa614416 was strongly homologous to a miniature inverted transposable element (MITE) stowaway-like element. Results of fluorescence in situ hybridization showed that the signal of pSa614416 was distributed along all S. cereale. cv Jingzhou chromosomes, but the signal strengths were unbalanced on the seven rye genome chromosomes. This repetitive element may be useful as a molecular marker for the introgression of rye germplasm into the wheat genome.

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“…Equal amounts of genomic DNA from 10 extremely short plants and 10 extremely tall plants from the F 2 mapping population were pooled to construct the dwarf bulk and tall bulk, respectively. SSR markers were synthesized based on sequence information from IPK Gatersleben (prefix: gwm and gdm; Röder et al, 1998;Pestsova et al, 2000), the Wheat Microsatellite Consortium (prefix: wmc; Gupta et al, 2002), the John Innes Centre (prefix: psp; Bryan et al, 1997), DuPont (prefix: dupw;Eujayl et al, 2002), R. Ward (prefix: barc; Song et al, 2005), INRA Clermont-Ferrand (prefix: cfd, cfa, and gpw;Guyomarc'h et al, 2002;Sourdille et al, 2003Sourdille et al, , 2005. PCR was performed in a volume of 10 μL in a Bio-Rad thermocycler.…”

Section: Dna Extraction and Molecular Marker Analysismentioning

confidence: 99%

Characterization and molecular mapping of a dwarf mutant in wheat

Jin

Sun

Li³

et al. 2013

Genet. Mol. Res.

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ABSTRACT.A spontaneous dwarf mutant of wheat was found in an F 5 generation line derived from a cross between Huamai No. 9 and Een No. 1 in 1998; it was named Huaai 01. We characterized the genetic pattern of Huaai 01 and mapped the gene controlling the dwarf trait. This dwarf mutant was found insensitive to exogenous gibberellic acid treatment, based on the length of the first leaf and the coleoptile at the seedling stage, suggesting that it plays a crucial role in the gibberellin response pathway. Genetic analysis revealed that a single gene that is partially recessive controls the dwarf phenotype in Huaai 01. We named the dwarfing gene Rht-B2. Simple sequence repeats (SSR) were examined as identifying markers linked to the Rht-B2 gene in an F 2 population. We screened 904 pairs of primers and identified 5 SSR markers linked to the Rht-B2 gene. Two markers, barc1096 and xgwm495, were located on the flanking region of the Rht-B2 gene at genetic distances of 2.9 and 3.3 cM, respectively. Based on published SSR linkage data for wheat, the Rht-B2 gene was mapped to the long arm of chromosome 4B. This identification and characterization of the Rht-B2 dwarfing gene will facilitate its utilization in wheat breeding.

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Microsatellite analysis of Aegilops tauschii germplasm

Cited by 100 publications

References 15 publications

Assessing genetic diversity of wheat (Triticum aestivum L.) germplasm using microsatellite markers

Assessing genetic diversity of wheat (Triticum aestivum L.) germplasm using microsatellite markers

Isolation and chromosomal localization of new MITE-like sequences from Secale

Characterization and molecular mapping of a dwarf mutant in wheat

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