MicroRNAs responding to southern rice black-streaked dwarf virus infection and their target genes associated with symptom development in rice

Xu, Donglin; Mou, Guiping; Wang, Kang; Zhou, Guohui

doi:10.1016/j.virusres.2014.07.007

Cited by 37 publications

(26 citation statements)

References 51 publications

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“…Recently, Xu et al . () used microarray technology to analyse miRNA expression patterns in black‐streaked dwarf virus (SRBSDV)‐infected rice and found 56 miRNAs, as well as 24 target genes, that are associated with symptom development. Next‐generation sequencing technology, which could rapidly identify almost all RNAs in cells, has been widely used for miRNA investigation, especially in finding novel or rice‐specific stress‐response miRNAs.…”

Section: Stress Tolerance‐related Micrornasmentioning

confidence: 99%

Application of microRNA gene resources in the improvement of agronomic traits in rice

Zheng

2015

Plant Biotechnology Journal

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Summary microRNAs (miRNAs) are important nonprotein-coding genes that are involved in almost all biological processes, including cell differentiation and fate determination, developmental regulation, and immune responses. Investigations have shown that some miRNAs can highly affect plant agricultural traits, including virus resistance, nematode resistance, drought and salinity tolerance, heavy metal detoxification, biomass yield, grain yield, fruit development and flower development. Therefore, these miRNAs are considered a newly identified gene resource for the genetic improvement of crops. In this review, we will summarize the recent findings of the rice miRNA-directed regulatory network, which controls agronomic traits such as yield, quality and stress tolerance, and explore the outlook for the uses of these miRNA-associated traits in rice biotechnology.

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Section: Stress Tolerance‐related Micrornasmentioning

confidence: 99%

Application of microRNA gene resources in the improvement of agronomic traits in rice

Zheng

2015

Plant Biotechnology Journal

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“…Viruses are economically important plant pathogens that typically cause symptoms of yellowing, mosaic, twisting and even necrosis of leaves, and plant death in many cases. Studies with many different viruses have shown that virus‐infected plants generally have a changed expression profile of miRNAs (Abreu et al ., ; Amin et al ., ; Bazzini et al ., ; Chen et al ., , ; Cillo et al ., ; Du et al ., ; Feng et al ., , ; Guo et al ., ; Lang et al ., , ; Liu et al ., ; Naqvi et al ., ; Pradhan et al ., ; Sun et al ., ; Wang et al ., ; Xu et al ., ; Yang et al ., ) and, in some cases, these changes are associated with viral symptoms (Gao et al ., ; Lian et al ., ; Romanel et al ., ). Bazzini et al.…”

Section: Introductionmentioning

confidence: 99%

Suppression of nbe‐miR166h‐p5 attenuates leaf yellowing symptoms of potato virus X on Nicotiana benthamiana and reduces virus accumulation

Wang

Cui

et al. 2018

Molecular Plant Pathology

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MicroRNAs (miRNAs) play essential roles in plant development. There is increasing evidence that changed expression of miRNAs in virus-infected plants contributes to the development of viral symptoms. Here, we analysed the altered expression of miRNAs of Nicotiana benthamiana in response to Potato virus X (PVX) by Illumina Solexa sequencing. One of the 21 miRNAs significantly affected, nbe-miR166h-p5, was closely associated with viral symptoms. Using the Tobacco rattle virus-based miRNA suppression (VbMS) system, we found that the suppression of nbe-miR166h-p5 in plants caused leaves to turn dark green with increased chlorophyll. When PVX was inoculated on nbe-miR166h-p5-suppressed plants, the leaf yellowing symptom of PVX was largely attenuated with less reduction in chlorophyll content, and the accumulation of PVX was decreased. nbe-miR166h-p5 was also up-regulated in plants infected by Turnip mosaic virus (TuMV), and its suppression attenuated the leaf yellowing symptom of TuMV and decreased viral accumulation. Three potential targets of nbe-miR166h-p5 were identified. The results indicate the association of nbe-miR166h-p5 with symptoms of PVX and also with those of TuMV, providing useful information on the relationship between miRNA and viral infection.

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“…and discussed correlations between the accumulation of the miRNAs and pathogenesis. Xu et al [33] investigated rice miRNAs responding to southern rice black-streaked dwarf virus (SRBSDV) infection and found that the expression levels of 56 miRNAs were altered in the diseased plants and that the miR164, miR396, miR530, miR1846 and miR1858 families in particular were associated with the development of symptoms. There have also been reports regarding the role of miRNAs in diseased cucumber.…”

Section: Introductionmentioning

confidence: 99%

Expression profiling and regulatory network of cucumber microRNAs and their putative target genes in response to cucumber green mottle mosaic virus infection

et al. 2019

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Cucumber green mottle mosaic virus (CGMMV) is an important pathogen of cucumber (Cucumis sativus). The molecular mechanisms mediating host-pathogen interactions are likely to be strongly influenced by microRNAs (miRNAs), which are known to regulate gene expression during the disease cycle. This study focused on 14 miRNAs (miR159, miR169, miR172, miR838, miR854, miR5658, csa-miRn1-3p, csa-miRn2-3p, csa-miRn3-3p, csa-miRn4-5p, csa-miRn5-5p, csa-miRn6-3p, csa-miRn7-5p and csa-miRn8-3p) and their target genes. The data collected was used to construct a regulatory network of miRNAs and target genes associated with cucumber-CGMMV interactions, which identified 608 potential target genes associated with all of the miRNAs except csa-miRn7-5p. Five of the miRNAs (miR159, miR838, miR854, miR5658 and csa-miRn6-3p) were found to be mutually linked by target genes, while another eight (miR169, miR172, csa-miRn1-3p, csa-miRn2-3p, csa-miRn3-3p, csa-miRn4-5p, csa-miRn5-5p and csa-miRn8-3p) formed subnetworks that did not display any connectivity with other miRNAs or their target genes. Reverse transcription quantitative real-time PCR (RT-qPCR) was used to analyze the expression levels of the different miRNAs and their putative target genes in leaf, stem and root samples of cucumber over a 42-day period after inoculation with CGMMV. A positive correlation was found between some of the miRNAs and their respective target genes, although for most, the response varied greatly depending on the time point, indicating that additional factors are likely to be involved in the interaction between cucumber miRNAs and their target genes. Several miRNAs, including miR159 and csa-miRn6-3p, were linked to target genes that have been associated with plant responses to disease. A model linking miRNAs, their targets and downstream biological processes is proposed to indicate the roles of these miRNAs in the cucumber-CGMMV pathosystem.Electronic supplementary materialThe online version of this article (10.1007/s00705-019-04152-w) contains supplementary material, which is available to authorized users.

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MicroRNAs responding to southern rice black-streaked dwarf virus infection and their target genes associated with symptom development in rice

Cited by 37 publications

References 51 publications

Application of microRNA gene resources in the improvement of agronomic traits in rice

Application of microRNA gene resources in the improvement of agronomic traits in rice

Suppression of nbe‐miR166h‐p5 attenuates leaf yellowing symptoms of potato virus X on Nicotiana benthamiana and reduces virus accumulation

Expression profiling and regulatory network of cucumber microRNAs and their putative target genes in response to cucumber green mottle mosaic virus infection

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