MicroRNAs Regulate Sleep and Sleep Homeostasis in Drosophila

Goodwin, Patricia R.; Meng, Alice; Moore, J K; Hobin, Michael; Fulga, Tudor A.; Vactor, David Van; Griffith, Leslie C.

doi:10.1016/j.celrep.2018.05.078

Cited by 39 publications

(30 citation statements)

References 31 publications

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“…In conclusion, consistent with prior animal experiments suggesting distinct roles for miRNAs in sleep-wake regulation and aging [14,15,17,[24][25][26][27][28], we here report for the first time that wakefulness and sleep affect the blood miRNA expression profile in young and older men. Given the rather small study sample, the reported results need to be considered preliminary.…”

Section: Discussionsupporting

confidence: 92%

“…A new line of research has recently linked miRNAs with sleep-wake regulation. More specifically, studies in Drosophila suggested that distinct miRNAs, particularly let-7, control the structure and amount of sleep, in addition to the sleep-wake dependent (i.e., homeostatic) aspect of sleep-wake regulation [17]. Sleep deprivation is the most pronounced physiological challenge of sleep homeostasis and drastically alters the blood proteome in animals and humans [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

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Prolonged Waking and Recovery Sleep Affect the Serum MicroRNA Expression Profile in Humans

et al. 2018

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MicroRNAs (miRNAs) are small, abundant, non-coding RNA fragments that regulate gene expression and silencing at the post-transcriptional level. The miRNAs each control various downstream targets and play established roles in different biological processes. Given that miRNAs were recently proposed to contribute to the molecular control of sleep–wake regulation in animal models and narcoleptic patients, we investigated the impact of acute sleep deprivation on blood miRNA expression in healthy adult men of two different age groups. Twenty-two young (mean age: 24 ± 3 years) and nine older (65 ± 1 years) volunteers completed a controlled in-lab study, consisting of 8 h baseline sleep, followed by 40 h of extended wakefulness, and a 10-h recovery sleep opportunity. At the same circadian time in all three conditions (at 4:23 p.m. ± 23 min), qPCR expression profiling of 86 miRNAs was performed in blood serum. Thirteen different miRNAs could be reliably quantified and were analyzed using mixed-model ANOVAs. It was found that miR-30c and miR-127 were reliably affected by previous sleep and wakefulness, such that expression of these miRNAs was upregulated after extended wakefulness and normalized after recovery sleep. Together with previous findings in narcolepsy patients, our preliminary data indicate that miR-30c and its target proteins may provide a biomarker of elevated sleep debt in humans.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Prolonged Waking and Recovery Sleep Affect the Serum MicroRNA Expression Profile in Humans

et al. 2018

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“…New epigenetic mechanisms regulating sleep are emerging, with several investigations showing that some miRNAs are related to circadian control (Goodwin et al., ). In our study, computational predictions suggest that selected miRNAs are potentially associated with the circadian rhythm in humans.…”

Section: Discussionmentioning

confidence: 99%

Circulating miRNAs are associated with sleep duration in children/adolescents: Results of the I.Family Study

Iacomino

Lauria

Russo

et al. 2020

Experimental Physiology

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It is commonly recognized that sleep is essential for children's health, and that insufficient sleep duration is associated with negative health outcomes. In humans, sleep duration and quality are influenced by genetic, environmental and social factors. Epigenetic mechanisms, likewise, regulate circadian rhythms and sleep patterns. In the present study, we aimed to identify circulating microRNAs associated with sleep duration in a subsample of normal-weight European children/adolescents (n = 111) participating in the I.Family Study. Subjects were divided into two groups based upon self-reported sleep duration, according to the recommended amount of sleep for paediatric populations. Sleep needs for children <13 years were at least 9 h per day, and for children >13 were at least 8 h per day. There were group differences (short sleepers versus normal sleepers) in circulating levels of miR-26b-3p (mean (95% CI) = 2.0 (1.3-2.7) versus 2.3 (1.9-2.7), P = 0.05) and miR-485-5p (mean (95% CI) = 0.6 (0.3-0.9) versus 0.9 (0.7 -1.0), P < 0.001), adjusting for country of origin, age, sex, pubertal status, screen time and highest educational level of parents. Our findings show for the first time that sleep duration reflects the profile of specific circulating microRNAs in school-aged children and adolescents. It is conceivable that epigenetic modifications, mainly related to circadian rhythm control, may be modulated or interfere with sleep duration.

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“…MiRSPs offer the ability to dissect the role of individual miRNAs with both spatial and temporal precision (Ebert and Sharp 2010;McNeill and Van Vactor 2012). The efficacy of this technique has been demonstrated in a number of studies in Drosophila and other species (Loya et al 2009;Bejarano et al 2012;Pathania et al 2012;Li et al 2013;Loya et al 2014;Lu et al 2014;Busto et al 2015;Fulga et al 2015;Mugat et al 2015;Goodwin et al 2018). More comprehensive comparison of adult viability phenotypes detected using our expanded toolkit of miR-SPs with data from similar screens of null alleles (Chen et al 2014) also shows that lethal phenotypes are rare and that miR-SPs recapitulate the vast majority of null phenotypes .…”

Section: Microrna Motor Behavior Drosophila Melanogastermentioning

confidence: 79%

MicroRNAs Regulate Multiple Aspects of Locomotor Behavior inDrosophila

Donelson

Dixit

Pichardo‐Casas

et al. 2020

G3 Genes|Genomes|Genetics

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Locomotion is an ancient and fundamental output of the nervous system required for animals to perform many other complex behaviors. Although the formation of motor circuits is known to be under developmental control of transcriptional mechanisms that define the fates and connectivity of the many neurons, glia and muscle constituents of these circuits, relatively little is known about the role of post-transcriptional regulation of locomotor behavior. MicroRNAs have emerged as a potentially rich source of modulators for neural development and function. In order to define the microRNAs required for normal locomotion in Drosophila melanogaster, we utilized a set of transgenic Gal4-dependent competitive inhibitors (microRNA sponges, or miR-SPs) to functionally assess ca. 140 high-confidence Drosophila microRNAs using automated quantitative movement tracking systems followed by multiparametric analysis. Using ubiquitous expression of miR-SP constructs, we identified a large number of microRNAs that modulate aspects of normal baseline adult locomotion. Addition of temperature-dependent Gal80 to identify microRNAs that act during adulthood revealed that the majority of these microRNAs play developmental roles. Comparison of ubiquitous and neural-specific miR-SP expression suggests that most of these microRNAs function within the nervous system. Parallel analyses of spontaneous locomotion in adults and in larvae also reveal that very few of the microRNAs required in the adult overlap with those that control the behavior of larval motor circuits. These screens suggest that a rich regulatory landscape underlies the formation and function of motor circuits and that many of these mechanisms are stage and/or parameter-specific.

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MicroRNAs Regulate Sleep and Sleep Homeostasis in Drosophila

Cited by 39 publications

References 31 publications

Prolonged Waking and Recovery Sleep Affect the Serum MicroRNA Expression Profile in Humans

Prolonged Waking and Recovery Sleep Affect the Serum MicroRNA Expression Profile in Humans

Circulating miRNAs are associated with sleep duration in children/adolescents: Results of the I.Family Study

MicroRNAs Regulate Multiple Aspects of Locomotor Behavior inDrosophila

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