MicroRNAs modulating nutrient homeostasis: a sustainable approach for developing biofortified crops

Jamla, Monica; Joshi, Shrushti; Patil, Suraj; Tripathi, Bhumi Nath; Kumar, Vinay

doi:10.1007/s00709-022-01775-w

Cited by 4 publications

(1 citation statement)

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“…The demand for P fertilizers worldwide in 2022 was predicted as high as 49.1 million tons 8 ; however excessive application of P fertilizer also caused severe problems such as environmental pollution 7 . Therefore, the development of a new crop variety with efficient P use is required for agricultural production in P-deficit soils 9,10 .…”

Section: Overexpression Of Soybean Microrna156b Enhanced Tolerance To...mentioning

confidence: 99%

Overexpression of soybean microRNA156b enhanced tolerance to phosphorus deficiency and seed yield in Arabidopsis

Tian

Hao

et al. 2023

Sci Rep

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AbstractmicroRNAs (miRNAs) are endogenous small RNAs that are key regulatory factors participating in various biological activities such as the signaling of phosphorus deficiency in the plant. Previous studies have shown that miR156 expression was modulated by phosphorus starvation in Arabidopsis and soybean. However, it is not clear whether the over-expression of soybean miR156b (GmmiR156b) can improve a plant’s tolerance to phosphorus deficiency and affect yield component traits. In this study, we generated Arabidopsis transgenic lines overexpressing GmmiR156b and investigated the plant’s response to phosphorus deficiency. Compared with the wild type, the transgenic Arabidopsis seedlings had longer primary roots and higher phosphorus contents in roots under phosphorus-deficit conditions, but lower fresh weight root/shoot ratios under either phosphorus-deficient or sufficient conditions. Moreover, the GmmiR156b overexpression transgenic lines had higher phosphorus content in shoots of adult plants and grew better than the wide type under phosphorus-deficient conditions, and exhibited increased seed yields as well as strong pleiotropic developmental morphology such as dwarfness, prolonged growth period, bushy shoot/branching, and shorter silique length, suggesting that the transgenic lines were more tolerant to phosphorus deficiency. In addition, the expression level of four SQUAMOSA PROMOTER BINDING PROTEIN LIKE (SPL) genes (i.e., AtSPL4/5/6/15) were markedly suppressed in transgenic plants, indicating that they were the main targets negatively regulated by GmmiR156b (especially AtSPL15) and that the enhanced tolerance to phosphorus deficiency and seed yield is conferred mainly by the miR156-mediated downregulation of AtSPL15.

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Section: Overexpression Of Soybean Microrna156b Enhanced Tolerance To...mentioning

confidence: 99%