MicroRNAs modulate core-clock gene expression in pancreatic islets during early postnatal life in rats

Jacovetti, Cécile; Rodríguez-Trejo, Adriana; Guay, Claudiane; Sobel, Jonathan; Gattesco, Sonia; Petrenko, Volodymyr; Saini, Camille; Dibner, Charna; Regazzi, Romano

doi:10.1007/s00125-017-4348-6

Cited by 29 publications

(25 citation statements)

References 53 publications

(73 reference statements)

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“…Indeed, these oscillators are not coupled even when cells are establishing tight physical contacts in confluent cell culture, as was demonstrated by co‐culture experiments between fibroblast cells bearing distinct oscillatory characteristics . In line with the reported cell‐autonomous nature of the peripheral oscillators, pronounced circadian oscillations were registered not only in isolated intact rodent and human pancreatic islets, but also in dispersed islet cells cultured in monolayer . These findings imply that physical interactions between neighbouring cells within the 3‐dimensional islet architecture may be dispensable for cellular oscillator function.…”

Section: Molecular Characterization Of Pancreatic Islet Circadian Clockssupporting

confidence: 54%

“…7 In line with the reported cell-autonomous nature of the peripheral oscillators, pronounced circadian oscillations were registered not only in isolated intact rodent and human pancreatic islets, but also in dispersed islet cells cultured in monolayer. 47,75,77,78 These findings imply that physical interactions between neighbouring cells within the 3-dimensional islet architecture may be dispensable for cellular oscillator function. Noteworthy, crosstalk between islet cellular oscillators might nevertheless be maintained via paracrine signalling molecules, secreted by adjacent endocrine cells, without the need of physical contact, as discussed in section 4.2.…”

Section: Body Metabolism Is Orchestrated By Circadian Clocks In Rodmentioning

confidence: 99%

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Time zones of pancreatic islet metabolism

Petrenko

Philippe

Dibner

2018

Diabetes Obesity Metabolism

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Most living beings possess an intrinsic system of circadian oscillators, allowing anticipation of the Earth's rotation around its own axis. The mammalian circadian timing system orchestrates nearly all aspects of physiology and behaviour. Together with systemic signals originating from the central clock that resides in the hypothalamic suprachiasmatic nucleus, peripheral oscillators orchestrate tissue-specific fluctuations in gene transcription and translation, and posttranslational modifications, driving overt rhythms in physiology and behaviour. There is accumulating evidence of a reciprocal connection between the circadian oscillator and most aspects of physiology and metabolism, in particular as the circadian system plays a critical role in orchestrating body glucose homeostasis. Recent reports imply that circadian clocks operative in the endocrine pancreas regulate insulin secretion, and that islet clock perturbation in rodents leads to the development of overt type 2 diabetes. While whole islet clocks have been extensively studied during the last years, the heterogeneity of islet cell oscillators and the interplay between α- and β-cellular clocks for orchestrating glucagon and insulin secretion have only recently gained attention. Here, we review recent findings on the molecular makeup of the circadian clocks operative in pancreatic islet cells in rodents and in humans, and focus on the physiologically relevant synchronizers that are resetting these time-keepers. Moreover, the implication of islet clock functional outputs in the temporal coordination of metabolism in health and disease will be highlighted.

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Section: Molecular Characterization Of Pancreatic Islet Circadian Clockssupporting

confidence: 54%

Section: Body Metabolism Is Orchestrated By Circadian Clocks In Rodmentioning

confidence: 99%

Time zones of pancreatic islet metabolism

Petrenko

Philippe

Dibner

2018

Diabetes Obesity Metabolism

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“…Several pathways previously related to the maturation process such as insulin secretion or circadian rhythms are under the control of these accessible cis-regulatory elements. Interestingly, several components of the core clock are not rhythmic in 10 days old pups but are consistently oscillating in β-cells after weaning (33). These cis-regulatory elements may be controlled by transcriptional enhancers or repressors.…”

Section: Discussionmentioning

confidence: 99%

Chromatin accessibility variations across pancreatic islet maturation

Sobel

Guay

Rodríguez-Trejo

et al. 2019

Preprint

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Glucose-induced insulin secretion, a peculiar property of fully mature β-cells, is only achieved after birth and is preceded by a phase of intense proliferation. These events occurring in the neonatal period are decisive for the establishment of an appropriate functional β-cell mass that provides the required insulin throughout life. However, key regulators of gene expression involved in cellular reprogramming along pancreatic islet maturation remain to be elucidated. The present study addressed this issue by mapping open chromatin regions in newborn versus adult rat islets using the ATAC-seq assay. Accessible regions were then correlated with the expression profiles of mRNAs to unveil the regulatory networks governing functional islet maturation. This led to the identification of Scrt1, a novel transcriptional repressor controlling β-cell proliferation. ATAC-seq | Scrt1 | Pancreatic Islet maturation | β-cellCorrespondence: Romano.Regazzi@unil.ch Sobel et al. | bioRχiv | September 25, 2019 | 1-17

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“…82 Beyond the circadian regulation at gene transcription level, microRNAs are subject to regulation by the clock in mouse liver and macrophages. 83,84 This connection is reciprocal, with microRNAs influencing clock maturation during development, 85 and components of the core clock machinery in the adult organism. 86,87 Regarding the clock regulation of protein synthesis, 88,89 circadian proteomic analyses unravel that a large fraction of rhythmic proteins are encoded by mRNAs whose accumulation pattern is arrhythmic.…”

Section: Of Mammalian Physiology and Metabolismmentioning

confidence: 99%

The importance of being rhythmic: Living in harmony with your body clocks

Dibner

2019

Acta Physiologica

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OSCILLATORSThe time-keeping system, dubbed "circadian" from Latin circa diem (about a day), allows light-sensitive beings, including humans, to coordinate their physiology and behaviour to the daily changes in geophysical time (Figure 1). The mammalian network of body clocks is organized in a strictly hierarchical manner. A master oscillator, residing in the paired suprachiasmatic nuclei (SCN) of the hypothalamus ( Figure 1A), synchronizes a myriad of peripheral oscillators situated in each organ ( Figure 1B) on a daily basis. In turn, rhythmicity in the SCN is entrained by external timecues or Zeitgebers (German term for time givers). Daily changes in light intensity, detected by classical photoreceptors and intrinsically light-sensitive retinal ganglion cells expressing AbstractCircadian rhythms have developed in all light-sensitive organisms, including humans, as a fundamental anticipatory mechanism that enables proactive adaptation to environmental changes. The circadian system is organized in a highly hierarchical manner, with clocks operative in most cells of the body ensuring the temporal coordination of physiological processes. Circadian misalignment, stemming from modern life style, draws increasing attention due to its tight association with the development of metabolic, cardiovascular, inflammatory and mental diseases as well as cancer.This review highlights recent findings emphasizing the role of the circadian system in the temporal orchestration of physiology, with a particular focus on implications of circadian misalignment in human pathologies. K E Y W O R D Scircadian clock, circadian misalignment, continuous recording of circadian bioluminescence, human physiology How to cite this article: Dibner C. The importance of being rhythmic: Living in harmony with your body clocks. Acta Physiol. 2020;228:e13281. https ://doi.

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MicroRNAs modulate core-clock gene expression in pancreatic islets during early postnatal life in rats

Cited by 29 publications

References 53 publications

Time zones of pancreatic islet metabolism

Time zones of pancreatic islet metabolism

Chromatin accessibility variations across pancreatic islet maturation

The importance of being rhythmic: Living in harmony with your body clocks

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