MicroRNAs Make a Difference in Cardiovascular Robustness

Bakker, Rachael; Carthew, Richard W.

doi:10.1016/j.devcel.2017.03.009

Cited by 3 publications

(3 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1 B ). Although it is well accepted that miRNAs confer robustness on gene expression (8, 38, 44), it is still not clear what aspects of their inhibitory mechanisms are used to gain efficient noise reduction (45), and previous analysis has focused on miRNAs that are not coexpressed with the target proteins (8). One hypothesis is that coexpression of miRNAs with their targets has a role in increasing robustness of gene expression (36).…”

Section: Discussionmentioning

confidence: 99%

Molecular Filters for Noise Reduction

Laurenti

Csikász‐Nagy

Kwiatkowska

et al. 2018

Biophysical Journal

View full text Add to dashboard Cite

Living systems are inherently stochastic and operate in a noisy environment, yet despite all these uncertainties, they perform their functions in a surprisingly reliable way. The biochemical mechanisms used by natural systems to tolerate and control noise are still not fully understood, and this issue also limits our capacity to engineer reliable, quantitative synthetic biological circuits. We study how representative models of biochemical systems propagate and attenuate noise, accounting for intrinsic as well as extrinsic noise. We investigate three molecular noise-filtering mechanisms, study their noise-reduction capabilities and limitations, and show that nonlinear dynamics such as complex formation are necessary for efficient noise reduction. We further suggest that the derived molecular filters are widespread in gene expression and regulation and, particularly, that microRNAs can serve as such noise filters. To our knowledge, our results provide new insight into how biochemical networks control noise and could be useful to build robust synthetic circuits.

show abstract

Section: Discussionmentioning

confidence: 99%

Molecular Filters for Noise Reduction

Laurenti

Csikász‐Nagy

Kwiatkowska

et al. 2018

Biophysical Journal

View full text Add to dashboard Cite

show abstract

“…17,18 miRNAs are involved in many biological and physiological processes, such as the regulation of disease formation, and embryonic development. [19][20][21][22] miRNAs are abundant in the CNS and are critically involved in all stages of neural differentiation during brain development. 23,24 miR-96 has been reported to repress neural induction from human embryonic stem cells (hESCs) by targeting Pax6, the critical regulator of neural differentiation.…”

Section: Introductionmentioning

confidence: 99%

“…MicroRNAs (miRNAs) are 20‐25 nucleotide (nt) non‐coding RNAs that partially bind to the mRNA 3′ untranslated regions (3′UTRs) to induce the translational repression . miRNAs are involved in many biological and physiological processes, such as the regulation of disease formation, and embryonic development . miRNAs are abundant in the CNS and are critically involved in all stages of neural differentiation during brain development .…”

Section: Introductionmentioning

confidence: 99%

LncRNA Riken‐201 and Riken‐203 modulates neural development by regulating the Sox6 through sequestering miRNAs

et al. 2019

View full text Add to dashboard Cite

Objectives: Long non-coding RNAs (LncRNAs) play important roles in epigenetic regulatory function during the development processes. In this study, we found that through alternative splicing, LncRNA C130071C03Riken variants and are both expressed highly in brain, and increase gradually during neural differentiation. However, the function of Rik-201 and Rik-203 is unknown. Materials and methods: Embryonic stem cells (ESCs); RNA sequencing; gene expression of mRNAs, LncRNAs and miRNAs; over-expression and RNA interference of genes; flow cytometry; real-time quantity PCR; and Western blot were used in the studies. RNA pull-down assay and PCR were employed to detect any miRNA that attached to Rik-201 and Rik-203. The binding of miRNA with mRNA of Sox6 was presented by the luciferase assay. Results: Repression of Rik-201 and Rik-203 inhibited neural differentiation from mouse embryonic stem cells. Moreover, Rik-201 and Rik-203 functioned as the competing endogenous RNA (ceRNA) to repress the function of miR-96 and miR-467a-3p, respectively, and modulate the expression of Sox6 to further regulate neural differentiation. Knockout of the Rik-203 and Rik-201 induced high ratio of brain developmental retardation. Further we found that C/EBPβ might potentially activated the transcription of Rik-201 and Rik-203. Conclusions: These findings identify the functional role of Rik-201 and Rik-203 in facilitating neural differentiation and further brain development, and elucidate the underlying miRNAs-Sox6-associated molecular mechanisms.

show abstract

RNAi-based therapeutics for lung cancer: biomarkers, microRNAs, and nanocarriers

Magalhães

Alvarez‐Lorenzo²,

Figueiras

et al. 2018

Expert Opinion on Drug Delivery

View full text Add to dashboard Cite

Despite the current advances in the discovery of the lung cancer biomarkers and, consequently, in the diagnosis, this pathology continues to be the primary cause of cancer-related death worldwide. In most cases, the illness is diagnosed in an advanced stage, which limits the current treatment options available and reduces the survival rate. Therefore, RNAi-based therapy arises as a promising option to treat lung cancer. Areas covered: This review provides an overview on the exploitation of lung cancer biology to develop RNAi-based therapeutics to be applied in the treatment of lung cancer. Furthermore, the review analyzes the main nanocarriers designed to deliver RNAi molecules and induce antitumoral effects in lung cancer, and provides updated information about current RNAi-based therapeutics for lung cancer in clinical trials. Expert opinion: RNAi-based therapy uses nanocarriers to perform a targeted and efficient delivery of therapeutic genes into lung cancer cells, by taking advantage of the known biomarkers in lung cancer. These therapeutic genes are key regulatory molecules of crucial cellular pathways involved in cell proliferation, migration, and apoptosis. Thereby, the characteristics and functionalization of the nanocarrier and the knowledge of lung cancer biology have direct influence in improving the therapeutic effect of this therapy.

show abstract

MicroRNAs Make a Difference in Cardiovascular Robustness

Cited by 3 publications

References 9 publications

Molecular Filters for Noise Reduction

Molecular Filters for Noise Reduction

LncRNA Riken‐201 and Riken‐203 modulates neural development by regulating the Sox6 through sequestering miRNAs

RNAi-based therapeutics for lung cancer: biomarkers, microRNAs, and nanocarriers

Contact Info

Product

Resources

About