MicroRNAs in spent blastocyst culture medium are derived from trophectoderm cells and can be explored for human embryo reproductive competence assessment

Capalbo, Antonio; Ubaldi, Filippo Maria; Cimadomo, Danilo; Noli, Laila; Khalaf, Yakoub; Farcomeni, Alessio; Ilić, Duško; Rienzi, Laura

doi:10.1016/j.fertnstert.2015.09.014

Cited by 148 publications

(150 citation statements)

References 56 publications

(59 reference statements)

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“…Specific miRNAs are also detectable in spent blastocyst culture medium, with correlations to oocyte insemination method, embryo ploidy, and live birth (Rosenbluth et al ., 2014). Recently, Capalbo et al (2016) have comprehensively characterized the population of miRNAs secreted from human blastocysts into spent culture medium, and two miRNAs (miR-20a, miR-30c) were positively correlated with blastocyst implantation.…”

Section: Introductionmentioning

confidence: 99%

miR-142-3p as a biomarker of blastocyst implantation failure - A pilot study

Borges¹,

Setti²,

Braga³

et al. 2016

JBRA Assisted Reproduction

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ObjectiveThis study aims to find whether microRNAs (miRNAs) detected in the culture medium of embryos produced in vitro could be potential biomarkers of embryo implantation.MethodsCulture media samples from 36 embryos, derived from patients undergoing intracytoplasmic sperm injection (ICSI) in a private university-affiliated IVF center, were collected between January/2015 and November/2015. Samples were collected on day three and embryo transfers were performed on day five and all embryos reached the blastocyst stage. Samples were split into groups according to the embryo implantation result: Positive-Implantation-Group (n=18) or Negative-Implantation-Group (n=18). For the first analysis, samples were pooled in three sets for each group (6-7 spent media per pool). MicroRNAs were extracted from spent media and cDNA was synthesized. C. elegans miR-39 was used as RNA spike-in to normalize the gene expression analysis. The expression of microRNAs into the spent media from the Positive-Implantation-Group was compared with those from the Negative-Implantation-Group. A set of seven miRNAs (miR-21, miR-142-3p, miR-19b, miR-92a, miR-20b, miR-125a and miR148a) selected according with the literature, was tested. To check whether miRNAs could be detected in individual samples of culture media, in a second analysis, ten more samples were tested for miR-21 and miR-142-3p.ResultsFrom the sevens tested miRNAs, a significant increased expression of miR-142-3p could be noted in the Negative-Implantation-Group (P<0.001). For other three miRNAs (miR-21, miR-19b and miR-92a) a difference in expression was observed, however it did not reach a statistical significance. In addition, when ten non-redundant samples were tested to check if miRNAs could be detected in individual samples of culture media, the highly specific amplification of mature miRNAs, including miR-142-3p, could be noted.ConclusionOur findings suggest that miR-142-3p, previously described as a tumor suppressor and cell cycle inhibitor, may be a potential biomarker of blastocyst implantation failure. The identification of miRNAs on individual culture medium samples offers unique opportunities for non-invasive early diagnosis of blastocyst implantation.

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Section: Introductionmentioning

confidence: 99%

miR-142-3p as a biomarker of blastocyst implantation failure - A pilot study

Borges¹,

Setti²,

Braga³

et al. 2016

JBRA Assisted Reproduction

View full text Add to dashboard Cite

show abstract

“…Nevertheless, this strategy need to be further explored, analyzing more types of miRNAs in larger experimental setting, before to being introduced in clinical routine. A prospective multicenter study is currently ongoing where a panel of 46 selected miRNAs will be used in order to confirm these preliminary findings on a higher and defined sample size (24).…”

Section: Micrornamentioning

confidence: 94%

“…Another pioneering embryo selection strategy could involve the characterization of extracellular microRNAs (miRNAs) secreted in spent culture media and their possible use as biomarkers (23,24). Over 1,000 miRNAs have been identified in human genome: they are stable, noncoding, single-strained RNA molecules and they are considered to be the major negative transcriptional regulators of gene expression.…”

Section: Micrornamentioning

confidence: 99%

“…In addition, one of them (miR-191) was also found in higher concentration in culture media from aneuploid compared to euploid embryos (28). Later, in a prospective cohort study, the miRNAs in the spent culture media from 25 implanted and 28 not implanted blastocysts after 12 hours of culture after warming, obtained in 44 consecutive IVF cycle, were analyzed (24). First of all, it was found that the majority of miRNAs detected in more than three TE biopsies and/or spent culture media samples were shared among all samples examined.…”

Section: Micrornamentioning

confidence: 99%

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Unconventional embryo selection strategies: a look into the future

Maria¹

2016

CCE

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SummaryThe assessment of an unequivocal method able to identify the embryo with the higher possibility to implant, leading to a single live birth, is crucial for the success of an infertility treatment. Standard morphology evaluation, morphokinetic analysis and preimplantation genetic screening are the more employed embryo selection strategies in reproductive medicine centers. However, the results and the applicability of these methodologies are still controversial. Therefore, there is the need to find additional technologies to clinically select developing embryos. The challenge is to set-up a predictive, not invasive, reproducible, objective and effective method able to select the best embryo. In this review, we present several unconventional methodologies of embryo evaluation, analyzing their possible future application in human reproduction.

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“…Based on bioinformatic analysis, miR-20a is predicted to regulate two transcripts (SOS1, TCF7L1) involved in endometrial cell proliferation and growth through the modulation of five genes (PTEN, NRAS, MAPK1, MYC, and CCND1). Also, miR30c is predicted to regulate five distinct transcripts (APC, KRAS, PIK3CD, SOS1, and FOXO3) that are involved in endometrial cell proliferation and growth [34].…”

Section: (Ii) Predictive Factors For Pregnancy Using Spent Culture Mementioning

confidence: 99%

Towards Further Optimization of Preimplantation Embryo Culture Media: from the Viewpoint of Omics and Somatic Cell Nuclear Transfer (SCNT) Studies

Yamada

Hamatani

Akutsu

et al. 2016

Journal of Mammalian Ova Research

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MicroRNAs in spent blastocyst culture medium are derived from trophectoderm cells and can be explored for human embryo reproductive competence assessment

Abstract: MicroRNAs secreted from human blastocysts in culture media can be profiled with high reproducibility, and this approach can be further explored for noninvasive embryo selection.

Cited by 148 publications

References 56 publications

miR-142-3p as a biomarker of blastocyst implantation failure - A pilot study

miR-142-3p as a biomarker of blastocyst implantation failure - A pilot study

Unconventional embryo selection strategies: a look into the future

Towards Further Optimization of Preimplantation Embryo Culture Media: from the Viewpoint of Omics and Somatic Cell Nuclear Transfer (SCNT) Studies

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