MicroRNAs in Platelets: Should I Stay or Should I Go?

Águila, Sonia; Cuenca-Zamora, Ernesto José; Martı́nez, Constantino; Teruel‐Montoya, Raúl

doi:10.5772/intechopen.93181

Cited by 3 publications

(2 citation statements)

References 112 publications

(109 reference statements)

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“…Since the largest pool of microvesicles is derived from platelets, a substantial ratio of cell-free miRNAs is transferred from activated platelets into other cell types and interfere with gene expression ( Águila et al, 2020 ). Based on former results, platelet-derived miR-223 regulated EFNA1 and FBXW7 gene expression at mRNA level in HUVECs ( Laffont et al, 2013 ), while others reported the inhibitory role of platelet miR-320b on endothelial ICAM-1 expression after myocardial infarction ( Gidlöf et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Platelet Microparticles Enriched in miR-223 Reduce ICAM-1-Dependent Vascular Inflammation in Septic Conditions

et al. 2021

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In the process of sepsis, activated platelets shed microvesicles containing microRNAs (miRNAs), which can be internalized by distinct recipient cells in circulation, consequently eliciting a potent capability to regulate their cellular functions in different diseases. In the present study, activated human platelets transferring miR-223 into endothelial cells via platelet-derived microparticles (PMPs) was investigated in vitro during septic conditions with a proposed mechanism involving in downregulation of the enhanced expression of intercellular adhesion molecule-1 (ICAM-1). The uptake of PMPs encasing miR-223 and the adhesion of peripheral blood mononuclear cells (PBMCs) on human coronary artery endothelial cells (HCAECs) were observed by immunofluorescence microscopy upon co-culture with PMPs isolated from sepsis or control plasma. The expression of miR-223-3p and its gene target ICAM1 in HCAECs were quantified by RT-qPCR and ELISA after the cells were incubated with septic or control PMPs, whose levels were induced with thrombin-receptor activating peptide (TRAP). Leukocyte-depleted platelets (LDPs) from septic patients showed a decreased miR-223 level, while septic plasma and PMPs revealed an elevated miRNA level compared to control samples. Similarly, TRAP-activated LDPs demonstrated a reduced intracellular miR-223 expression, while increased levels in the supernatant and PMP isolates were observed vs. untreated samples. Furthermore, TNF-α alone resulted in decreased miR-223 and elevated ICAM1 levels in HCAECs, while PMPs raised the miRNA level that was associated with downregulated ICAM1 expression at both mRNA and protein levels under TNF-α treatment. Importantly, miR-223 was turned out not to be newly synthesized as shown in unchanged pre-miR-223 level, and mature miR-223 expression was also elevated in the presence of PMPs in HCAECs after transfection with Dicer1 siRNA. In addition, septic PMPs containing miR-223 decreased ICAM1 with a reduction of PBMC binding to HCAECs. In conclusion, septic platelets released PMPs carrying functional miR-223 lower ICAM1 expression in endothelial cells, which may be a protective role against excessive sepsis-induced vascular inflammation.

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Section: Discussionmentioning

confidence: 99%

Platelet Microparticles Enriched in miR-223 Reduce ICAM-1-Dependent Vascular Inflammation in Septic Conditions

et al. 2021

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“…Since the largest pool of microvesicles is derived from platelets, a substantial ratio of cell-free miRNAs is transferred from activated platelets to other cell types and interfere with gene expression. 65 As these regulatory processes can be targeted by silencing RNA molecules as well as mimics, it is important to characterize the miRNA changes during sepsis so that these processes can be appropriately interfered. We have described in a series of septic patients by a TaqMan OpenArray technique that out of 390 platelet miRNAs, 121 were significantly decreased and 61 were upregulated in sepsis versus controls.…”

Section: A Potential Novel Pathway In Interfering With Sepsis Induced...mentioning

confidence: 99%

Inflammation, Sepsis, and the Coagulation System

Kappelmayer,

Debreceni,

Fejes

et al. 2024

Hamostaseologie

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Sepsis has been a major health problem for centuries and it is still the leading cause of hospital deaths. Several studies in the past decades have identified numerous biochemical abnormalities in severe cases, and many of these studies provide evidence of the perturbation of the hemostatic system. This can result in complications, such as disseminated intravascular coagulation that can lead to multiorgan failure. Nevertheless, large clinical studies have demonstrated that the simple approach of inhibiting the coagulation processes by any means fails to provide significant improvement in the survival of septic patients. A cause of this failure could be the fact that in sepsis the major clinical problems result not primarily from the presence of the infective agent or enhanced coagulation but from the complex dysregulated systemic host response to pathogens. If this overt reaction is not fully deciphered, appropriate interference is highly unlikely and any improvement by conventional therapeutic interventions would be limited. Cellular activation in sepsis can be targeted by novel approaches like inhibition of the heterotypic cellular interactions of blood cells by targeting surface receptors or posttranscriptional control of the hemostatic system by noncoding ribonucleic acid (RNA) molecules. Stable RNA molecules can affect the expression of several proteins. Thus, it can be anticipated that modulation of microRNA production would result in a multitude of effects that may be beneficial in septic cases. Here, we highlight some of the recent diagnostic possibilities and potential novel routes of the dysregulated host response.

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Insight into microRNAs’ involvement in hematopoiesis: current standing point of findings

Nassiri,

Ahmadi Afshar,

Almasi

2023

Stem Cell Res Ther

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Hematopoiesis is a complex process in which hematopoietic stem cells are differentiated into all mature blood cells (red blood cells, white blood cells, and platelets). Different microRNAs (miRNAs) involve in several steps of this process. Indeed, miRNAs are small single-stranded non-coding RNA molecules, which control gene expression by translational inhibition and mRNA destabilization. Previous studies have revealed that increased or decreased expression of some of these miRNAs by targeting several proto-oncogenes could inhibit or stimulate the myeloid and erythroid lineage commitment, proliferation, and differentiation. During the last decades, the development of molecular and bioinformatics techniques has led to a comprehensive understanding of the role of various miRNAs in hematopoiesis. The critical roles of miRNAs in cell processes such as the cell cycle, apoptosis, and differentiation have been confirmed as well. However, the main contribution of some miRNAs is still unclear. Therefore, it seems undeniable that future studies are required to focus on miRNA activities during various hematopoietic stages and hematological malignancy.

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MicroRNAs in Platelets: Should I Stay or Should I Go?

Cited by 3 publications

References 112 publications

Platelet Microparticles Enriched in miR-223 Reduce ICAM-1-Dependent Vascular Inflammation in Septic Conditions

Platelet Microparticles Enriched in miR-223 Reduce ICAM-1-Dependent Vascular Inflammation in Septic Conditions

Inflammation, Sepsis, and the Coagulation System

Insight into microRNAs’ involvement in hematopoiesis: current standing point of findings

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