MicroRNAs in pemphigus and pemphigoid diseases

Papara, Cristian; Zillikens, Detlef; Sadik, Christian D.; Baican, Adrian

doi:10.1016/j.autrev.2021.102852

Cited by 7 publications

(5 citation statements)

References 134 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Twelve miRNAs (miR-125b-5p, miR-146a-5p, miR-148a-3p, miR-150-5p, miR-155-5p, miR-181a-5p, miR-181b-5p, miR-326, miR-338-3p, miR-423-5p, miR-424-5p, and miR-584-5p) were selected based on recent studies about autoimmune diseases and miRNAs [ 14 , 22 , 23 ]. The 12 miRNAs were detected by qRT-PCR in the PV and NC groups.…”

Section: Resultsmentioning

confidence: 99%

“…Some studies have revealed miRNAs’ expression profiles in autoimmune blistering diseases. For example, miR-424-5p is overexpressed in the peripheral blood mononuclear cells of pemphigus patients; miR-99, miR-125b, and miR-181 are increased in the keratinocytes of Hailey–Hailey disease (HHD) lesions; some miRNAs, including miR-27a-5p, miR-223, miR-379, miR-423-5p, and miR-1291, are significantly altered in pemphigoid diseased [ 14 ]. However, the studies have mainly focused on pemphigus foliaceus, paraneoplastic pemphigus, HHD or pemphigoid diseases, and there are some knowledge gaps regarding miRNAs in PV, especially in the mucosal-dominant variant.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Identification of Six microRNAs as Potential Biomarkers for Pemphigus Vulgaris: From Diagnosis to Pathogenesis

He¹,

Xing

Li³

et al. 2022

Diagnostics

View full text Add to dashboard Cite

Background: Pemphigus vulgaris (PV) is a potentially fatal autoimmune bullous disease. The role of microRNA (miRNA, miR) in the diagnosis and pathogenesis of PV remains unknown. This study aims to provide potential miRNA biomarkers for PV diagnosis and therapy options. Methods: Serum samples were obtained from 22 PV patients, 15 mucous membrane pemphigoid (MMP) patients, and 10 normal controls (NC). Total RNA was extracted from the serum samples, and 12 selected miRNAs were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Bioinformatic analyses including target gene prediction and enrichment analysis were performed. Results: Twelve miRNAs were increased in the serum of the PV group compared with the NC group, in which six miRNAs had good efficacy to diagnose PV from MMP with the area under the receiver operator characteristic curves of 0.970 to 0.988. A series test for the combination of miR-584-5p and miR-155-5p reached the sensitivity and specificity of 95.5% and 100%. Bioinformatic analysis revealed target gene enrichment in the cell adhesion pathways, immune-relating pathways, and P38 mitogen-activated protein kinases signaling pathway. Conclusion: The study provides new insights and targets of miRNAs for the precise diagnosis and the exploration of pathogenesis for PV, which may serve as a reference for further research into autoimmune bullous diseases.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification of Six microRNAs as Potential Biomarkers for Pemphigus Vulgaris: From Diagnosis to Pathogenesis

He¹,

Xing

Li³

et al. 2022

Diagnostics

View full text Add to dashboard Cite

show abstract

“…В последние годы активно изучается экспрессия ми кроРНК в качестве нового метода диагностики при раз личных дерматологических заболеваниях [44,45], в том числе при аутоиммунной пузырчатке [46]. МикроРНК представляют собой малые некодирующие РНК, контро лирующие экспрессию генов на посттранскрипционном уровне.…”

Section: новые подходы в диагностике и оценке тяжести пузырчатки микр...unclassified

Pemphigus: New approaches to diagnosis and disease severity assessment

Teplyuk,

Kolesova,

Mak

et al. 2023

Russian Journal of Skin and Venereal Diseases

View full text Add to dashboard Cite

Pemphigus represents a group of severe and potentially fatal autoimmune bullous diseases. Pemphigus clinical findings represents skin and/or mucous membranes keratinocytes acantholysis with intraepithelial blisters and erosions formation which is life-threatening and, if relapsed, significantly reduce the patients quality of life. The on-time disease diagnosis is a key to successful pemphigus management and timely treatment onset allowing intensive disease progression avoidance. The article reviews up-to-date diagnosing methods and assessment measure for monitoring pemphigus severity. In addition to standard diagnosis methods, such as clinical findings, histological examination, direct and indirect immunofluorescence, and enzyme immunoassay (ELISA), the article focuses on revieing new diagnostic and disease severity prediction approaches, such as microRNAs expression and skin and mucous membranes microbiome analysis since false positive and false negative results in histological and immunohistochemical analysis as well as the laboratories insufficient equipment makes finding pemphigus new diagnostic and prognostic methods a relevant challenge.

show abstract

“…Dysregulated expression of miR‐17, miR‐21, miR‐146a and miR‐155 has been reported in cutaneous disorders, either in the lesional skin or within peripheral blood mononuclear cells (PBMCs) from patients with atopic dermatitis (AD), psoriasis and cutaneous lupus 16–18 . However, data regarding miRNA characterization in pemphigus and pemphigoid are scarce 19,20 …”

Section: Introductionmentioning

confidence: 99%

“…[16][17][18] However, data regarding miRNA characterization in pemphigus and pemphigoid are scarce. 19,20 In this study, we aimed to gain deeper insights into the potential role of miRNAs as biomarkers for PF by characterizing the expression profiles of miR-17, miR-21, miR-146a, miR-155 and miR-338 using quantitative PCR in PBMCs and lesional skin samples from categorized PF patients and healthy controls (HCs). Additionally, we conducted a clinical follow-up to investigate miRNA expression changes during therapy.…”

mentioning

confidence: 99%

miRNA implication in the pathogenesis and the outcome of Tunisian endemic pemphigus foliaceus

Khabou

Fakhfakh

Tahri

et al. 2023

Experimental Dermatology

View full text Add to dashboard Cite

Pemphigus foliaceus (PF) is a bullous autoimmune skin disease diagnosed through sera and skin analyses. PF severity is associated with maintained anti‐Dsg1 sera levels and its prognosis is unpredictable. MicroRNA (miRNA), dynamic regulators of immune function, have been identified as potential biomarkers for some autoimmune diseases. This study aimed to assess the miRNA expression of miR‐17‐5p, miR‐21‐5p, miR‐146a‐5p, miR‐155‐5p and miR‐338‐3p using quantitative real‐time PCR in peripheral blood mononuclear cells (PBMC) and lesional skin samples from untreated and treated PF patients (both remittent and chronic) over 3 months. Overall, miRNA expression was significantly higher in PBMC than in biopsy samples. Blood miR‐21 expression was increased in untreated patients compared to controls and had a diagnostic value with an AUC of 0.78. After 6 weeks, it decreased significantly, similar to anti‐Dsg1 antibodies and the PDAI score. In addition, a positive correlation was observed between cutaneous miR‐21 expression and the disease activity score. Conversely, cutaneous expressions of miR‐17, miR‐146a and miR‐155 were significantly higher in treated chronic patients compared to remittent ones. The cutaneous level of miR‐155 positively correlated with pemphigus activity, making it a potential predictive marker for patients' clinical stratification with an AUC of 0.86.These findings suggest that blood miR‐21 and cutaneous miR‐155 can be used as supplemental markers for PF diagnosis and activity, respectively in addition to classical parameters.

show abstract

MicroRNAs in pemphigus and pemphigoid diseases

Cited by 7 publications

References 134 publications

Identification of Six microRNAs as Potential Biomarkers for Pemphigus Vulgaris: From Diagnosis to Pathogenesis

Identification of Six microRNAs as Potential Biomarkers for Pemphigus Vulgaris: From Diagnosis to Pathogenesis

Pemphigus: New approaches to diagnosis and disease severity assessment

miRNA implication in the pathogenesis and the outcome of Tunisian endemic pemphigus foliaceus

Contact Info

Product

Resources

About