MicroRNAs in large herpesvirus DNA genomes: recent advances

Sorel, Océane; Dewals, Benjamin G

doi:10.1515/bmc-2016-0017

Cited by 24 publications

(19 citation statements)

References 111 publications

(121 reference statements)

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“…microRNAs (miRNAs/miRs) are a class of endogenous and highly conserved single-stranded non-coding small RNAs of 19-22 nucleotides in length. They are able to inhibit target gene expression at the post-transcriptional level through specifically binding with the 3'-untranslated region (3'-UTR) of their target mRNAs, thus exerting biological functions (4,5). Studies have verified that miRNAs have important regulatory roles in cell proliferation, apoptosis, development and apoptosis, processes that are also closely associated with tumor pathogenesis, development and prognosis (6,7).…”

Section: Introductionmentioning

confidence: 99%

miR‑940 inhibits cell proliferation and promotes apoptosis in esophageal squamous cell carcinoma cells and is associated with post‑operative prognosis

Wang¹,

Song²,

Qiao³

et al. 2019

Exp Ther Med

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The present study aimed to examine microRNA (miR)-940 expression in esophageal squamous cell carcinoma (ESCC) tissues and cells, analyze its association with the clinicopathological features and prognosis of patients, and explore the potential underlying mechanisms. miR-940 expression in ESCC cell lines and a normal esophageal cell line was detected using reverse transcription-quantitative (RT-q)PCR. Furthermore, 210 resected ESCC tissue and para-carcinoma tissue specimens were collected, and miR-940 expression in those tissues was detected by RT-qPCR. In addition, the association of miR-940 with the clinicopathological features and prognosis of patients was analyzed. In an in vitro experiment, miR-940 mimics were transduced into ESCC cells by the liposome method. An MTT assay was used to detect the effect of miR-940 on the viability of ESCC cells. The influence of miR-940 on the cell cycle and apoptotic rate of ESCC cells was detected by flow cytometry. The present results indicated that the expression levels of miR-940 in human ESCC tissues and cell lines were markedly downregulated, and that low expression of miR-940 in ESCC tissues was significantly associated with a poor degree of differentiation, positive lymph node metastasis and advanced clinical stage. Kaplan-Meier survival analysis suggested that low miR-940 expression was associated with poor prognosis. Cox regression analysis revealed that lymph node metastasis, clinical stage and miR-940 expression were independent risk factors affecting the prognosis of patients. Overexpression of miR-940 in ESCC cells markedly reduced the cell viability, blocked the cell cycle at G0/G1 phase and promoted cell apoptosis. These results suggest that miR-940 is downregulated in ESCC, which is linked to the occurrence and progression of ESCC. Conversely, overexpression of miR-940 reduced the cell viability and promoted apoptosis of ESCC cells. Therefore, miR-940 may be a promising novel prognostic marker and anti-cancer target in ESCC.

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Section: Introductionmentioning

confidence: 99%

miR‑940 inhibits cell proliferation and promotes apoptosis in esophageal squamous cell carcinoma cells and is associated with post‑operative prognosis

Wang¹,

Song²,

Qiao³

et al. 2019

Exp Ther Med

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“…In latent stage, KSHV also encodes 12 pre‐miRNAs that produce 25 mature microRNAs in addition to a handful of latent proteins, and these miRNAs are highly expressed during viral latency and in KS tumors, implying their essential functions in the viral life cycle and the development of KS tumors. The KSHV miRNAs, including miR‐K5, miR‐K7‐5p, miR‐K9‐5p, miR‐K3, and miR‐K4, have been shown to promote latency by directly or indirectly targeting KSHV lytic switch protein (RTA) . Another viral miRNA that also contributes to latency is miR‐K1, which leads to NF‐κB‐dependent viral latency by targeting IκBα .…”

Section: Introductionmentioning

confidence: 99%

Kaposi's sarcoma‐associated herpesvirus (KSHV)‐encoded microRNAs promote matrix metalloproteinases (MMPs) expression and pro‐angiogenic cytokine secretion in endothelial cells

Guo

Wan

Qin

et al. 2017

Journal of Medical Virology

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The human oncogenic virus Kaposi's sarcoma-associated herpesvirus (KSHV) is linked to Kaposi's sarcoma (KS), a tumor of endothelial cells characterized by angiogenesis and invasiveness. KSHV genome encodes 25 mature microRNAs (miRNAs), but their roles in KSHV-induced tumor dissemination and angiogenesis are not fully understood. In this study, we constructed the sensor reporters of KSHV miRNAs and used a luciferase reporter assay to demonstrate the function of the mimics of KSHV miRNAs. Then, we examined the expression of matrix metalloproteinases (MMPs) and pro-angiogenic cytokines that are related to cell migration and angiogenesis in the KSHV 25 miRNAs transfected endothelial cells. We found that all KSHV miRNAs increased the expression of the transcripts of MMP1, MMP13, VEGFA, and VEGFR2 in different degrees, as well as the secretion of VEGFA protein in the supernatant of endothelial cells. Our results reveal that KSHV miRNAs contribute to regulating MMPs and expression of pro-angiogenic factors, thus, suggesting that these miRNAs might play a crucial role in KSHV-induced cell motility and angiogenesis.

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“…MicroRNAs (miRNAs) represent small RNA species composed by~20-14 nucleotides found in virtually all living organisms (Grey 2015;Sorel and Dewals 2016). Mature miRNAs are able to cause either mRNA degradation or translational repression regulating physiological process by target genes after their transcription (Glazov et al 2009).…”

Section: Introductionmentioning

confidence: 99%

“…Recently, induction of miR-155 was associated to HSV1 acute encephalitis in a mouse model (Majer et al 2017). Considering the finding described for HSV1 encephalitis (Shivkumar et al 2013;Bhela et al 2014;Grey 2015;Sorel and Dewals 2016;Majer et al 2017), it would be worth to investigate miRNA expression in the CNS during BHV5 infection.…”

Section: Introductionmentioning

confidence: 99%

Expression of miR-155 associated with Toll-like receptors 3, 7, and 9 transcription in the olfactory bulbs of cattle naturally infected with BHV5

Oliveira

Vieira

et al. 2017

J. Neurovirol.

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Bovine herpesvirus 5 (BHV5) infection of young cattle is frequently associated with fatal neurological disease and, as such, represents an attractive model for studying the pathogenesis of viral-induced meningoencephalitis. Following replication in the nasal mucosa, BHV5 invades the central nervous system (CNS) mainly through the olfactory pathway. The innate immune response triggered by the host face to virus replication through the olfactory route is poorly understood. Recently, an upregulation of conserved pathogen-associated molecular pattern, as Toll-like receptors (TLRs), has been demonstrated in the CNS of BHV5 experimentally infected cows. A new perspective to understand host-pathogen interactions has emerged elucidating microRNAs (miRNAs) network that interact with innate immune response during neurotropic viral infections. In this study, we demonstrated a link between the expression of TLRs 3, 7, and 9 and miR-155 transcription in the olfactory bulbs (OB) of 16 cows suffering from acute BHV5-induced neurological disease. The OBs were analyzed for viral antigens and genome, miR-155 and TLR 3, 7, and 9 expression considering three major regions: olfactory receptor neurons (ORNs), glomerular layer (GL), and mitral cell layer (ML). BHV5 antigens and viral genomes, corresponding to glycol-C gene, were detected in all OBs regions by fluorescent antibody assay (FA) and PCR, respectively. TLR 3, 7, and 9 transcripts were upregulated in ORNs and ML, yet only ORN layers revealed a positive correlation between TLR3 and miR-155 transcription. In ML, miR-155 correlated positively with all TLRs studied. Herein, our results evidence miR-155 transcription in BHV5 infected OB tissue associated to TLRs expression specifically ORNs which may be a new window for further studies.

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MicroRNAs in large herpesvirus DNA genomes: recent advances

Cited by 24 publications

References 111 publications

miR‑940 inhibits cell proliferation and promotes apoptosis in esophageal squamous cell carcinoma cells and is associated with post‑operative prognosis

miR‑940 inhibits cell proliferation and promotes apoptosis in esophageal squamous cell carcinoma cells and is associated with post‑operative prognosis

Kaposi's sarcoma‐associated herpesvirus (KSHV)‐encoded microRNAs promote matrix metalloproteinases (MMPs) expression and pro‐angiogenic cytokine secretion in endothelial cells

Expression of miR-155 associated with Toll-like receptors 3, 7, and 9 transcription in the olfactory bulbs of cattle naturally infected with BHV5

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