MicroRNAs in equine veterinary science

Hollis, A. R.; Starkey, Mike

doi:10.1111/evj.12954

Cited by 10 publications

(4 citation statements)

References 109 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Physiological and pathological roles of miRNA in animal diseases has been the subject of intensive research for only a few years. MicroRNAs are involved in a broad spectrum animal disease, among which the following ones should be mentioned above all: cancer, metabolic and infection diseases and immune defense [76,77] (Table 2). Cancer.…”

Section: Micrornas As Potential Biomarkers For Veterinary Researchmentioning

confidence: 99%

The role of microRNAs in animal physiology and pathology

Szczepanek

Pareek

Tretyn

2018

TRVS

View full text Add to dashboard Cite

MicroRNAs are a class of small, evolutionarily conserved, endogenous RNAs, capable of controlling gene expression. MicroRNAs are transcribed by RNA polymerases II and III, generating precursors that undergo a series of cleavage events to form mature microRNA. They play an important regulatory role in animals at the posttranscriptional levels by targeting mRNAs for direct cleavage of mRNAs or repression of mRNA translation. The main biological function of miRNA is the post-translation regulation of cells, like: proliferation and differentiation, cell death, fat metabolism, neuronal patterning and angiogenesis. These molecules are the main regulators of biological features of economic

show abstract

Section: Micrornas As Potential Biomarkers For Veterinary Researchmentioning

confidence: 99%

The role of microRNAs in animal physiology and pathology

Szczepanek

Pareek

Tretyn

2018

TRVS

View full text Add to dashboard Cite

show abstract

“…Some miRNAs can be excreted in the plasma, packed in exosomes, or bound to proteins. 23 Similar to the measurement of gene expression, the measurement of the cell-free miRNAs expression in the plasma seems suitable for doping control, especially because the starting material, plasma, is easy to collect and store. In humans, circulating miRNAs were demonstrated as potential doping markers particularly for erythropoietin (EPO), 24 AAS, 25 GH 26 administrations as well as for blood doping.…”

Section: Introductionmentioning

confidence: 99%

“…The miRNAs are small non‐coding RNAs that regulate gene expression. Some miRNAs can be excreted in the plasma, packed in exosomes, or bound to proteins 23 . Similar to the measurement of gene expression, the measurement of the cell‐free miRNAs expression in the plasma seems suitable for doping control, especially because the starting material, plasma, is easy to collect and store.…”

Section: Introductionmentioning

confidence: 99%

miRNAs detection in equine plasma by quantitative polymerase chain reaction for doping control: Assessment of blood sampling and study of eca‐miR‐144 as potential erythropoiesis stimulating agent biomarker

Loup¹,

André²,

Avignon³

et al. 2021

Drug Testing and Analysis

View full text Add to dashboard Cite

Short half‐life doping substances are, quickly eliminated and therefore difficult to control with traditional analytical chemistry methods. Indirect methods targeting biomarkers constitute an alternative to extend detection time frames in doping control analyses. Gene expression analysis (i.e., transcriptomics) has already shown interesting results in both humans and equines for erythropoietin (EPO), growth hormone (GH), and anabolic androgenic steroid (AAS) misuses. In humans, circulating cell‐free microRNAs in plasma were described as new potential biomarkers for control of major doping agent (MDA) abuses. The development of a quantitative polymerase chain reaction (qPCR) method allowing the detection of circulating miRNAs was carried out on equine plasma collected on different type of tubes (EDTA, lithium‐heparin [LiHep]). Although analyzing plasma collected in EDTA tubes is a standard method in molecular biology, analyzing plasma collected in LiHep tubes is challenging, as heparin is a reverse transcription (RT) and a PCR inhibitor. Different strategies were considered, and attention was paid on both miRNAs extraction quality and detection sensitivity. The detection of endogenous circulating miRNAs was performed and compared between the different types of tubes. In parallel, homologs of human miRNAs characterized as potential biomarkers of doping were sought in equine databases. The miRNA eca‐miR‐144, described as potential erythropoiesis stimulating agents (ESAs) administration candidate biomarker was retained and assessed in equine post‐administration samples. The results about the qPCR method development and optimization are exposed as well as the equine miRNAs detection. To our knowledge, this work is the first study and the proof of concept of circulating miRNAs detection in plasma dedicated to equine doping control.

show abstract

“…By targeting the three prime untranslated region (3′-UTR) region of protein-coding genes, miRNAs affect the expression of target genes, thereby influencing cell growth, proliferation, differentiation, apoptosis, and other biological behaviors. [9,10] Numerous studies have demonstrated that miRNAs are implicated in the pathogenesis of ALI. [11] Reportedly, miRNA-1246 suppresses ALI-induced inflammation and apoptosis via the NF-κB and Wnt/β-catenin pathways.…”

Section: Introductionmentioning

confidence: 99%

Hesperetin ameliorates lipopolysaccharide‐induced acute lung injury via the miR‐410/SOX18 axis

Dong

Zhou

Zhao

et al. 2020

J Biochem & Molecular Tox

View full text Add to dashboard Cite

Hesperetin (Hesp), a dihydroflavone, has a wide range of pharmacological activities, including antioxidant, anti-inflammatory, and antitumor effects, as well as cardiovascular protection. It also has protective effects against acute lung injury (ALI); however, the exact mechanism remains unclear. In the present study, the protective effects and mechanism of Hesp in the lungs were investigated. Hematoxylin and eosin staining was used to examine pathological changes in the lungs. Enzyme-linked immunosorbent assay was used to detect proinflammatory cytokine levels. In addition, reverse transcriptionquantitative polymerase chain reaction and Western blot analysis were used to observe the transcription and translation changes in the related genes, respectively. The results indicate that Hesp not only improves histopathological changes in the lungs but decreases the wet/dry ratio. In addition, total cell counts and the number of neutrophils and macrophages were lower in the bronchoalveolar fluid after Hesp treatment, consistent with the change in proinflammatory cytokine levels. MicroRNA-410 (miR-410) levels were significantly lower in the lung tissues of ALI mice and were reversed after Hesp treatment. Furthermore, miR-410 overexpression due to injection with agomiR-410 produced similar protective effects as Hesp. However, blocking miR-410 inhibited the protective effects of Hesp in the lungs of ALI mice. In addition, miR-410 has been shown to target the inhibition of sex determining region Y-box 18 (SOX18), indicating that Hesp might alleviate inflammatory secretion by blocking the miR-410/SOX18 axis. Thus, Hesp might be a potential agent for the treatment of ALI.

show abstract

MicroRNAs in equine veterinary science

Cited by 10 publications

References 109 publications

The role of microRNAs in animal physiology and pathology

The role of microRNAs in animal physiology and pathology

miRNAs detection in equine plasma by quantitative polymerase chain reaction for doping control: Assessment of blood sampling and study of eca‐miR‐144 as potential erythropoiesis stimulating agent biomarker

Hesperetin ameliorates lipopolysaccharide‐induced acute lung injury via the miR‐410/SOX18 axis

Contact Info

Product

Resources

About