MicroRNAs in colorectal cancer: Role in metastasis and clinical perspectives

Muhammad, Shan; Kaur, Kavanjit; Huang, Rui; Zhang, Qian; Kaur, Parvinder; Yazdani, Hamza O.; Bilal, Muhammad; Jiang, Zheng; Zheng, Liduan; Wang, Xishan

doi:10.3748/wjg.v20.i45.17011

Cited by 51 publications

(57 citation statements)

References 92 publications

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“…In the past, miRNAs have been found to be frequently deregulated in CRC, and some miRNAs were found to be associated with particular clinicopathological features of CRC, such as metastasis, recurrence and prognosis [17][18][19]. Moreover, compelling evidence indicates that miRNAs may play important roles in CRC progression and may directly contribute to the proliferation, avoidance of apoptosis and metastasis of CRC [20,21].…”

Section: Discussionmentioning

confidence: 99%

miR-142-3p Suppresses Cell Growth by Targeting CDK4 in Colorectal Cancer

Zhu

Yang

et al. 2018

Cell Physiol Biochem

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Background/Aims: Deregulation of microRNAs (miRNAs) has been associated with a variety of cancers, including colorectal cancer (CRC). Here, we investigated anomalous miR-142-3p expression and its possible functional consequences in primary CRC samples. Methods: The expression of miR-142-3p was measured by quantitative RT-PCR in 116 primary CRC tissues and adjacent non-tumor tissues. The effect of miR-142-3p up- or down-regulation in CRC-derived cells was evaluated in vitro by cell viability and colony formation assays and in vivo by growth assays in xenografted nude mice. Results: Using quantitative RT-PCR, we found that miR-142-3p was down-regulated in 78.4 % (91/116) of the primary CRC tissues tested when compared to the adjacent non-tumor tissues. We also found that the miR-142-3p mimic reduced in vitro cell viability and colony formation by inducing cell cycle arrest in CRC-derived cells, and inhibited in vivo tumor cell growth in xenografted nude mice. Inversely, we found that the miR-142-3p inhibitor increased the viability and colony forming capacity of CRC-derived cells and tumor cell growth in xenografted nude mice. In addition, we identified CDK4 as a potential target of miR-142-3p by predictions and dual-luciferase reporter assays. Concordantly, we found that miR-142-3p mimics and inhibitors could decrease and increase CDK4 protein levels in CRC-derived cells, respectively. Conclusion: From our results we conclude that miR-142-3p may act as a tumor suppressor in CRC and may serve as a tool for miRNA-based CRC therapy.

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Section: Discussionmentioning

confidence: 99%

miR-142-3p Suppresses Cell Growth by Targeting CDK4 in Colorectal Cancer

Zhu

Yang

et al. 2018

Cell Physiol Biochem

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“…Increasing evidence has indicated that the deregulation and dysfunction of miRNAs is key in the development and progression of CRC (22,23). Increased expression of oncomiRs and decreased expression of tsmiRs have been observed in CRC tissues as compared with adjacent non-tumor tissues (24).…”

Section: Discussionmentioning

confidence: 99%

miR-517a is an independent prognostic marker and contributes to cell migration and invasion in human colorectal cancer

Jiang

et al. 2016

Oncology Letters

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Abstract. Colorectal cancer (CRC) is a highly invasive tumor that is frequently associated with distant metastasis, which is the primary cause of poor prognosis. However, the mechanisms of metastasis remain poorly understood. MicroRNAs (miRNAs/miRs) have been considered to be implicated in CRC progression. In particular, miR-517a is proposed as a novel tumor-associated miRNA and has a potential role in tumor metastasis. The expression of miR-517a in CRC specimens was detected by reverse transcription-quantitative polymerase chain reaction. Transwell assays were performed to determine the migration and invasion of CRC cells. The putative target genes of miR-517a were disclosed using publicly available databases and western blot analysis. The present study identified that the expression of miR-517a was significantly higher in CRC tissues as compared with adjacent non-tumor tissues. Clinical analysis indicated that increased expression of miR-517a was correlated with poor prognostic features and poor long-term survival of CRC patients. In vitro evidences demonstrated that downregulation of miR-517a inhibited cell migration and invasion in HCT-116 cells. By contrast, upregulation of miR-517a increased the number of migrated and invaded SW480 cells. Notably, miR-517a expression was inversely regulated by forkhead box J3 (FOXJ3) abundance in CRC cells. Furthermore, an inverse correlation between miR-517a and FOXJ3 expression was observed in CRC tissues. In conclusion, miR-517a appears to be an independent prognostic marker for predicting survival of CRC patients, and may promote cell migration and invasion by inhibiting FOXJ3.

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“…12 In CRC, several studies have reported that miRNAs are involved in tumor progression and metastasis and are therefore suggested as potential prognostic biomarkers. 13,14 It has been demonstrated that the transcription factor TWIST-1 induces miR-10b which in turn inhibits the translation of a member of the homeobox (HOX) gene family, HOXD10. The expression of HOXD10 is lost during the malignant progression of breast cancer, resulting in the increased levels of the pro-metastatic gene RHOC.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of miR-10b in colorectal cancer patients: Correlation with TWIST-1 and E-cadherin expression

et al. 2017

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MicroRNAs are emergent players of epigenetics that function as oncogenes or tumor suppressors and that have been implicated in regulating diverse cellular pathways. MiR-10b is an oncogenic microRNA involved in tumor invasion and metastasis in various cancers. Our data have shown that miR-10b is overexpressed in colorectal cancer samples in comparison with non-tumorous adjacent mucosa (p = 0.0025) and that it is associated with severe features such as tumor size >5 cm (p = 0.023), distant metastasis (p = 0.0022), non-differentiated tumors (p = 0.016), and vascular invasion (p = 0.01). Regarding the regulation of its expression, positive correlation between the loss of miR-10b and aberrant DNA methylation (p = 0.02) as well as a loss of TWIST-1 messenger RNA (p = 0.018) have been observed. Furthermore, expression analysis of the downstream miR-10b targets has shown that there are associations between low HOXD10 messenger RNA and E-cadherin protein levels (p < 0.0001, p = 0.0008, respectively) and overexpression of miR-10b. Our data suggests that overexpression of miR-10b results from high levels of TWIST-1 and may induce a decrease of E-cadherin membranous protein levels, thus contributing to the acquisition of metastatic phenotypes in colorectal cancer.

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MicroRNAs in colorectal cancer: Role in metastasis and clinical perspectives

Cited by 51 publications

References 92 publications

miR-142-3p Suppresses Cell Growth by Targeting CDK4 in Colorectal Cancer

miR-142-3p Suppresses Cell Growth by Targeting CDK4 in Colorectal Cancer

miR-517a is an independent prognostic marker and contributes to cell migration and invasion in human colorectal cancer

Overexpression of miR-10b in colorectal cancer patients: Correlation with TWIST-1 and E-cadherin expression

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