MicroRNAs <i>miR-146a1, miR-155_2,</i> and <i>miR-200a1</i> Are Regulated in Autoimmune Thyroid Diseases

Bernecker, C.; Lenz, Luisa; Ostapczuk, Martin; Schinner, Sven; Willenberg, Holger S.; Ehlers, Margret; Vordenbäumen, Stefan; Feldkamp, Joachim; Schott, M.

doi:10.1089/thy.2012.0277

Cited by 48 publications

(38 citation statements)

References 4 publications

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“…Circulating miR-146a levels markedly declined in SLE compared with healthy controls [24]. MiR-146a was significantly decreased in the thyroid tissue of GD [25]. By contrast our results indicated that there was no significant difference in circulating miR-146a expression between GD group and control group in plasma, and that the circulating miR-146a expression in GO patients was not only significantly reduced but also negatively correlated with the clinical activity of GO.…”

Section: Figcontrasting

confidence: 69%

Circulating levels of miR-146a and IL-17 are significantly correlated with the clinical activity of Graves’ ophthalmopathy

et al. 2014

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Section: Figcontrasting

confidence: 69%

Circulating levels of miR-146a and IL-17 are significantly correlated with the clinical activity of Graves’ ophthalmopathy

et al. 2014

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“…Wang et al indicated that circulating miR-146a levels were markedly decreased in systemic lupus erythematosus compared with healthy controls [29]. Bernecker et al indicated that miR-146a was significantly decreased in the thyroid tissue of Graves diseases (GD) [30]. Intriguingly, Wei et al indicated that the miR-146a expression in plasma of GD group was not notably different from that of the control group, and the miR-146a expression in GO patients was not only significantly reduced but also negatively correlated with the clinical activity of GO [3].…”

Section: Discussionmentioning

confidence: 99%

Mechanism of MicroRNA-146a/Notch2 Signaling Regulating IL-6 in Graves Ophthalmopathy

Wang

Chen

Long

2017

Cell Physiol Biochem

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Background/Aims: We intended to investigate the significance of microRNA-146a, Notch2 and IL-6 on Graves ophthalmopathy (GO) and the relationships among them. Methods: About 27 GO patients were incorporated in this study, including 13 patients with inactive GO and14 patients with active GO. Another 15 patients who had previously received strabismus orthopedics or ophthalmectomy due to trauma were selected as the control population. QRT-PCR assay was used to detect microRNA-146a and Notch2 expression levels in plasma. MTT assay and flow cytometry were respectively used to assess the viability and mitosis of the fibroblasts isolated from orbital connective tissue. Double antibody sandwich enzyme-linked immunosorbent assay (ELISA) was employed to detect serum IL-6 levels. The dual luciferase reporter gene assay was used to verify the targeting relationship between microRNA-146a and Notch2. Results: Compared with the control group, the relative expression of miR-146a was significantly increased whereas the relative expression of Notch2 was significantly decreased (all P < 0.05) in GO patients compare with the control. Notch2 can be directly targeted by microRNA-146a. The over-expression of miR-146a markedly facilitated Orbital Fibroblasts (OFs) viability and mitosis whereas markedly suppressed cell apoptosis (all P < 0.05). Exogenous microRNA-146a mimics could down-regulat the expression of Notch2 and up-regulate IL-6 (P < 0.05). The inhibition of microRNA-146 resulted in the elevated expression of Notch2 and decreased expression of IL-6 (P < 0.05). Conclusion: MicroRNA-146a may increase the IL-6 levels and exacerbate GO by directly targeting Notch2.

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“…Studies have indicated that miR-146a plays an important role in the pathogenesis of several autoimmune disorders, such as rheumatoid arthritis, systemic lupus erythematosus, and multiple sclerosis [16-18]. miR-146a was also proven to be associated with the development of autoimmune thyroid diseases [19, 20]. miR-146a targets multiple genes in the Toll-like receptor 4 (TLR4)/nuclear factor-κB (NF-κB) pathway, an important immune and inflammatory response pathway, that regulates the inflammatory response [21].…”

Section: Introductionmentioning

confidence: 99%

Circulating MiR-146a May be a Potential Biomarker of Coronary Heart Disease in Patients with Subclinical Hypothyroidism

Xiaohui

Zhang

et al. 2018

Cell Physiol Biochem

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Background/Aims: Subclinical hypothyroidism (SCH) plays a crucial role in the development and progression of coronary heart disease (CHD). However, any associated changes in the circulating microRNAs (miRNAs) levels and slightly elevated thyroid stimulating hormone (TSH) levels in CHD patients are unknown. miR-146a is a well known miRNA associated with inflammatory autoimmune diseases. Here, we evaluated miR-146a expression in patients, with the goal of re-evaluating the effect of SCH on CHD. Methods: A total of 192 study subjects who underwent coronary angiography for either suspected or confirmed CHD were enrolled in 3 groups: CHD with SCH, CHD alone, and healthy controls. The circulating levels of miR-146a were quantified using qRT-PCR. Results: Levels of miR-146a were positively correlated with CHD severity, as indicated by the Gensini score (r=0.354). The relative expression of miR-146a in the CHD+SCH, CHD and healthy control groups was 2.223±0.827, 1.588±0.726 and 0.632±0.309, respectively. Plasma TSH levels were positively correlated with miR-146a levels (r=0.321). According to multivariate logistic regression analyses, miR-146a levels were associated with the incidence of CHD in patients with SCH. For diagnosing CHD, the area under the ROC curve (AUC) of miR-146a and TSH was 0.779 and 0.752, respectively. When the TSH and miR-146a levels were combined to form a composite panel, the AUC of the panel was 0.858. Conclusion: Plasma miR-146a levels correlated with the severity of coronary atherosclerosis and increased with TSH slightly elevated in patients with CHD. Thus, miR-146a may have good predictive value for CHD among individuals with elevated TSH levels.

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MicroRNAs miR-146a1, miR-155_2, and miR-200a1 Are Regulated in Autoimmune Thyroid Diseases

Cited by 48 publications

References 4 publications

Circulating levels of miR-146a and IL-17 are significantly correlated with the clinical activity of Graves’ ophthalmopathy

Circulating levels of miR-146a and IL-17 are significantly correlated with the clinical activity of Graves’ ophthalmopathy

Mechanism of MicroRNA-146a/Notch2 Signaling Regulating IL-6 in Graves Ophthalmopathy

Circulating MiR-146a May be a Potential Biomarker of Coronary Heart Disease in Patients with Subclinical Hypothyroidism

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