MicroRNAs as prognostic markers for survival in renal cell carcinoma conventional type T 2-4.

Dotan, Zohar; Fridman, Eduard; Spector, Yael; Barshack, Iris; Chajut, Ayelet; Rosenwald, Shai; Gilad, S.; Catane, Rafael; Meiri, Eti; Berger, Rachel L.; Aharonov, Ranit; Ezra, E.; Ramon, J.

doi:10.1200/jco.2011.29.15_suppl.e21115

Cited by 2 publications

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“…In this pathological process, specific miRNAs function as oncomiRs or tumor suppressive miRNAs via their direct targets. Recent studies further demonstrated that miRNA expression signature served as prognostic biomarkers for cancer patients ( 24 - 26 ). However, the role of specific miRNAs, such as miR-210, in the chemotherapy or chemoresistance was not defined.…”

Section: Discussionmentioning

confidence: 99%

Cisplatin chemotherapy-induced miRNA-210 signaling inhibits hepatocellular carcinoma cell growth

Shi²

2019

Transl. Cancer Res

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Background Chemotherapy has improved the survival of hepatocellular carcinoma (HCC) patients, but the underlying mechanisms are still not fully understood. MicroRNAs (miRNAs) are critical regulators in carcinogenesis and involved in response to cancer therapy. However, the correlation between miRNAs and chemotherapy is not well-established, and the detailed mechanisms and responsive targets remain unclear. Here, we investigated the function and mechanism of miR-210 in HCC chemotherapy with cisplatin. Methods This study involved samples from patients after HCC surgery, including tumor and non-tumor liver tissues. Total RNA was extracted from the fresh tissue samples and the levels of miR-210 were assessed by qRT-PCR analysis. Cisplatin treatment was performed in HepG2 and PLC cell lines, and ephrin A3 (EFNA3) levels were determined by Western blotting. Results We observed that miR-210 expression was up-regulated in HCC tissues and correlated with HCC progression. Notably, HCC patients underwent recurrences after chemotherapy showed high levels of miR-210 expression in tumors, indicating that miR-210 might be involved in regulating the chemotherapeutic efficacy. We also demonstrated that cisplatin treatment decreased the expression of miR-210 and increased the expression of miR-210 target EFNA3 in HCC cells. Moreover, miR-210 overexpression prevented the effects of cisplatin and rescued HCC cell growth, and miR-210 inhibition contributed to improved chemosensitivity of cisplatin in HCC cells. Conclusions Our findings defined a novel mechanism underlying the efficacious effects of cisplatin chemotherapy in HCC, and miR-210-induced EFNA3 signaling might be a potential target of cisplatin in HCC treatment.

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Section: Discussionmentioning

confidence: 99%

Cisplatin chemotherapy-induced miRNA-210 signaling inhibits hepatocellular carcinoma cell growth

Shi²

2019

Transl. Cancer Res

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“…18 Previous studies have shown that miR-21 plays an important role in both RCC growth and invasion. 19,20 Studies have shown that the higher the level of expression of miR-21, the greater the invasive ability of RCC cells, 21,22 and at least two studies showed that elevated expression of miR-21 was associated with poor survival in RCC patients. 23 miR-21 and its target programmed cell death 4 (PDCD4) will provide a strong candidate for therapeutic applications in renal cancer in the future.…”

Section: Introductionmentioning

confidence: 99%