MicroRNAs as immune regulators and biomarkers in tuberculosis

Wang, Lulu; Xiong, Yan; Fu, Binying; Guo, Dong; Zaky, Mohamed Y.; Lin, Xiaoyuan; Wu, Haibo

doi:10.3389/fimmu.2022.1027472

Cited by 12 publications

(9 citation statements)

References 249 publications

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“…Additionally, miRNAs can be delivered into or out of cells by vesicles, which could be detected in body fluids ( 44 ). In addition, miRNA profiles can be related to disease development or division among diseases with identical symptoms ( 45 , 46 ), demonstrating their clinical significance.…”

Section: The Functions Of Exosomal Ncrnasmentioning

confidence: 99%

Functions of exosomal non-coding RNAs to the infection with Mycobacterium tuberculosis

Wang

et al. 2023

Front. Immunol.

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Tuberculosis (TB) is a major infectious disease induced by Mycobacterium tuberculosis (M. tb) which causes the world’s dominant fatal bacterial contagious disease. Increasing studies have indicated that exosomes may be a novel option for the diagnosis and treatment of TB. Exosomes are nanovesicles (30-150 nm) containing lipids, proteins and non-coding RNAs (ncRNAs) released from various cells, and can transfer their cargos and communicate between cells. Furthermore, exosomal ncRNAs exhibit diagnosis potential in bacterial infections, including TB. Additionally, differential exosomal ncRNAs regulate the physiological and pathological functions of M. tb-infected cells and act as diagnostic markers for TB. This current review explored the potential biological roles and the diagnostic application prospects of exosomal ncRNAs, and included recent information on their pathogenic and therapeutic functions in TB.

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Section: The Functions Of Exosomal Ncrnasmentioning

confidence: 99%

Functions of exosomal non-coding RNAs to the infection with Mycobacterium tuberculosis

Wang

et al. 2023

Front. Immunol.

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“…In the realm of molecular biology, gene regulation plays a crucial role in maintaining cellular function and has been implicated in the pathogenesis of complex diseases like PTB 12 . Noncoding RNAs (ncRNAs), particularly long noncoding RNAs (lncRNAs) and microRNAs (miRNAs), have emerged as significant players in the immune response to PTB 13 . LncRNAs, for instance, are key regulators in the inflammatory response to infection, particularly influencing T cell responses 14 , while miRNAs have been increasingly recognized for modulating host-TB bacilli interactions through immune pathways 15 .…”

Section: Introductionmentioning

confidence: 99%

Construction of ceRNA regulatory networks for active pulmonary tuberculosis

Li,

Xin,

Liu

et al. 2024

Sci Rep

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Delayed diagnosis in patients with pulmonary tuberculosis (PTB) often leads to serious public health problems. High throughput sequencing was used to determine the expression levels of lncRNAs, mRNAs, and miRNAs in the lesions and adjacent health lung tissues of patients with PTB. Their differential expression profiles between the two groups were compared, and 146 DElncRs, 447 DEmRs, and 29 DEmiRs were obtained between lesions and adjacent health tissues in patients with PTB. Enrichment analysis for mRNAs showed that they were mainly involved in Th1, Th2, and Th17 cell differentiation. The lncRNAs, mRNAs with target relationship with miRNAs were predicted respectively, and correlation analysis was performed. The ceRNA regulatory network was obtained by comparing with the differentially expressed transcripts (DElncRs, DEmRs, DEmiRs), then 2 lncRNAs mediated ceRNA networks were established. The expression of genes within the network was verified by quantitative real-time PCR (qRT-PCR). Flow cytometric analysis revealed that the proportion of Th1 cells and Th17 cells was lower in PTB than in controls, while the proportion of Th2 cells increased. Our results provide rich transcriptome data for a deeper investigation of PTB. The ceRNA regulatory network we obtained may be instructive for the diagnosis and treatment of PTB.

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“…The increased expression of circAGFG1 in alveolar lavage fluid of ATB patients can inhibit the apoptosis of M. tb-infected macrophages and promote autophagy through the circAGFG1/miR-1257/Notch2 axis ( Shi et al, 2020 ). NcRNAs also have the potential to serve as diagnostic markers, therapeutic targets or therapeutic evaluation indicators for TB ( Davuluri and Chauhan, 2022 ; Hemati et al, 2022 ; Liang et al, 2022 ; Wang L. et al, 2022 ; Wang Q. et al, 2022 ; Zhang et al, 2022 ). Studies found that the upregulated hsa_circ_0001953, hsa_circ_0009024, hsa_circ_051239, hsa_circ_029965 and hsa_circ_404022 and the downregulated hsa_circ_0001380 and hsa_circRNA_103571 in the serum and the downregulated NONHSAT101518.2, NONHSAT067134.2, NONHSAT148822.1, and NONHSAT078957.2 in serum exosomes could be used as potential biomarkers for patients with ATB disease ( Huang et al, 2018 ; Yi et al, 2018 ; Luo et al, 2020 ; Fang et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Analysis of the noncoding RNA regulatory networks of H37Rv- and H37Rv△1759c-infected macrophages

Tan

Wang²,

Wang³

et al. 2023

Front. Microbiol.

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Noncoding RNAs regulate the process of Mycobacterium tuberculosis (M. tb) infecting the host, but there is no simultaneous transcriptional information of long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) and the global regulatory networks of non-coding RNA. Rv1759c, a virulence factor, is a member of protein family containing the proline-glutamic acid (PE) in M. tb, which can increase M. tb survival. To reveal the noncoding RNA regulatory networks and the effect of Rv1759c on non-coding RNA expression during M. tb infection, we collected samples of H37Rv- and H37Rv△1759c-infected macrophages and explored the full transcriptome expression profile. We found 356 mRNAs, 433 lncRNAs, 168 circRNAs, and 12 miRNAs differentially expressed during H37Rv infection, 356 mRNAs, 433 lncRNAs, 168 circRNAs, and 12 miRNAs differentially expressed during H37Rv△1759c infection. We constructed lncRNA/circRNA-miRNA-mRNA regulatory networks during H37Rv and H37Rv△1759c infection. We demonstrated the role of one of the hubs of the networks, hsa-miR-181b-3p, for H37Rv survival in macrophages. We discovered that the expression changes of 68 mRNAs, 92 lncRNAs, 26 circRNAs, and 3 miRNAs were only related to the deletion of Rv1759c by comparing the transcription profiles of H37Rv and H37Rv△1759c. Here, our study comprehensively characterizes the transcriptional profiles in THP1-derived-macrophages infected with H37Rv and H37Rv△1759c, which provides support and new directions for in-depth exploration of noncoding RNA and PE/PPE family functions during the infection process.

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MicroRNAs as immune regulators and biomarkers in tuberculosis

Cited by 12 publications

References 249 publications

Functions of exosomal non-coding RNAs to the infection with Mycobacterium tuberculosis

Functions of exosomal non-coding RNAs to the infection with Mycobacterium tuberculosis

Construction of ceRNA regulatory networks for active pulmonary tuberculosis

Analysis of the noncoding RNA regulatory networks of H37Rv- and H37Rv△1759c-infected macrophages

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