MicroRNAs are essential for differentiation of the retinal pigmented epithelium and maturation of adjacent photoreceptors

Ohana, Reut; Weiman-Kelman, B.; Raviv, S.; Tamm, Ernst R.; Pasmanik-Chor, M; Rinon, Ariel; Netanely, Dvir; Shamir, Raanan; Solomon, Arieh S.; Ashery‐Padan, Ruth

doi:10.1242/jcs.177584

Cited by 12 publications

(16 citation statements)

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“…Many of the studied microRNAs have been suggested to be essential, e.g. miR-206 and miR-1a for skeletal muscle myoblast differentiation (Chen et al, 2010; Dey et al, 2011), miR-144/451 for erythroid cells differentiation (Dore et al, 2008; Rasmussen et al, 2010), miR-17∼92 during B lymphopoiesis and lung development (Ventura et al, 2008), miR-15a-1 and miR-18a for development and function of inner ear hair cells in vertebrates (Friedman et al, 2009), miR-219 for normal oligodendrocyte differentiation and myelination (Dugas et al, 2010), miR-204 for differentiation of the retinal pigmented epithelium (Ohana et al, 2015) and miR-375 for human spinal motor neuron development (Bhinge et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

miR-206 family is important for mitochondrial and muscle function, but not essential for myogenesis in vitro

Przanowska

Sobierajska

et al. 2019

Preprint

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AbstractmiR-206, miR-1a-1 and miR-1a-2 are induced during differentiation of skeletal myoblasts and promote myogenesis in vitro. miR-206 is required for skeletal muscle regeneration in vivo. Although this microRNA family is hypothesized to play an essential role in differentiation, a triple knockout of the three genes has not been done to test this hypothesis. We report that triple KO C2C12 myoblasts generated using CRISPR/Cas9 method differentiate despite the expected de-repression of the microRNA targets. Surprisingly, their mitochondrial function is diminished. Triple KO mice demonstrate partial embryonic lethality, most likely due to the role of miR-1a in cardiac muscle differentiation. Two triple KO mice survive and grow normally to adulthood with smaller myofiber diameter and diminished physical performance. Thus, unlike other microRNAs important in other differentiation pathways, the miR-206 family is not absolutely essential for myogenesis and is instead a modulator of optimal differentiation of skeletal myoblasts.

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Section: Introductionmentioning

confidence: 99%

miR-206 family is important for mitochondrial and muscle function, but not essential for myogenesis in vitro

Przanowska

Sobierajska

et al. 2019

Preprint

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“…The term 'pigmentosa' deals with the characteristic appearance, during the advanced states of the disease, of abnormal areas of pigment in the retina. Much evidence supports the role of miRNAs in normal retinal development and functions [8]. Alterations of miRNA regulation in conditional Dicer mouse mutant eyes reduce and damage normal development of lens, cornea, retina and optic chiasm [9].…”

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confidence: 99%

miRNAexpression profile of retinal pigment epithelial cells under oxidative stress conditions

et al. 2018

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Deep analysis of regulative mechanisms of transcription and translation in eukaryotes could improve knowledge of many genetic pathologies such as retinitis pigmentosa ( RP ). New layers of complexity have recently emerged with the discovery that ‘junk’ DNA is transcribed and, among these, miRNAs have assumed a preponderant role. We compared changes in the expression of mi RNA s obtained from whole transcriptome analyses, between two groups of retinal pigment epithelium ( RPE ) cells, one untreated and the other exposed to the oxidant agent oxidized low‐density lipoprotein (ox LDL ), examining four time points (1, 2, 4 and 6 h). We found that 23 mi RNA s exhibited altered expression in the treated samples, targeting genes involved in several biochemical pathways, many of them associated to RP for the first time, such as those mediated by insulin receptor signaling and son of sevenless. Moreover, five RP causative genes ( KLHL7 , RDH11 , CERKL , AIPL1 and USH1G ) emerged as already validated targets of five altered mi RNA s (hsa‐miR‐1307, hsa‐miR‐3064, hsa‐miR‐4709, hsa‐miR‐3615 and hsa‐miR‐637), suggesting a tight connection between induced oxidative stress and RP development and progression. This mi RNA expression analysis of oxidative stress‐induced RPE cells has discovered new regulative functions of mi RNA s in RP that should lead to the discovery of new ways to regulate the etiopathogenesis of RP .

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“…MicroRNA sequencing ranked miR-204 as the second most abundant miRNA (after miR-184) during lens development (E15-P9) with miR-204-5p representing the dominant form of processed miR-204 [132]. In mouse retina, Mir204 transcripts are widely expressed in subpopulations of cells in the GCL, particularly Muller glia, INL, and in the RPE [93,139,146,147,151,[155][156][157][159][160][161][162][163]. MicroRNA sequencing analysis ranks miR-204 as the sixth most highly expressed miRNA in the mouse retina and the fourth most abundant in the mouse RPE/choroid [131].…”

Section: Mouse Trpm3_mir204mentioning

confidence: 99%

“…In developing mouse RPE, conditional loss of the RNase III nuclease Dicer1, which cleaves pre-miRNAs, resulted in significant depletion (~11-fold) of miR-204 along with upregulation of several predicted target genes including Meis2 [162]. Dicer1-deficient RPE in vivo did not exhibit overt changes in cell morphology, identity, or fate (e.g., EMT) but, instead, was associated with increased cell density, reduced cell size, and arrested development of adjoining photoreceptors due to failed assembly of outer segment disk membranes.…”

Section: Mir-204 In Rpementioning

confidence: 99%

TRPM3_miR-204: a complex locus for eye development and disease

Shiels

2020

Hum Genomics

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First discovered in a light-sensitive retinal mutant of Drosophila, the transient receptor potential (TRP) superfamily of non-selective cation channels serve as polymodal cellular sensors that participate in diverse physiological processes across the animal kingdom including the perception of light, temperature, pressure, and pain. TRPM3 belongs to the melastatin sub-family of TRP channels and has been shown to function as a spontaneous calcium channel, with permeability to other cations influenced by alternative splicing and/or non-canonical channel activity. Activators of TRPM3 channels include the neurosteroid pregnenolone sulfate, calmodulin, phosphoinositides, and heat, whereas inhibitors include certain drugs, plant-derived metabolites, and G-protein subunits. Activation of TRPM3 channels at the cell membrane elicits a signal transduction cascade of mitogen-activated kinases and stimulus response transcription factors. The mammalian TRPM3 gene hosts a non-coding microRNA gene specifying miR-204 that serves as both a tumor suppressor and a negative regulator of post-transcriptional gene expression during eye development in vertebrates. Ocular co-expression of TRPM3 and miR-204 is upregulated by the paired box 6 transcription factor (PAX6) and mutations in all three corresponding genes underlie inherited forms of eye disease in humans including early-onset cataract, retinal dystrophy, and coloboma. This review outlines the genomic and functional complexity of the TRPM3_miR-204 locus in mammalian eye development and disease.

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MicroRNAs are essential for differentiation of the retinal pigmented epithelium and maturation of adjacent photoreceptors

Cited by 12 publications

References 1 publication

miR-206 family is important for mitochondrial and muscle function, but not essential for myogenesis in vitro

miR-206 family is important for mitochondrial and muscle function, but not essential for myogenesis in vitro

miRNAexpression profile of retinal pigment epithelial cells under oxidative stress conditions

TRPM3_miR-204: a complex locus for eye development and disease

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