MicroRNAs and Their Regulatory Role in Sugarcane

Swapna, M.; Kumar, Sanjeev

doi:10.3389/fpls.2017.00997

Cited by 18 publications

(12 citation statements)

References 39 publications

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“…In the present study, miR531 was differentially expressed in both hybrid and male parent, while none of miRNA was differentially expressed in both hybrid and female parent. Previous researches have showed that miR531 plays important roles in drought response and innate immunity in rice, biomass improvement in sugarcane [54], and metabolism in switchgrass [55]. Additionally, miR531 is involved in signal transduction pathways in rice [56].…”

Section: Hybrid Wheat Showed Heterosis In Seed Vigormentioning

confidence: 99%

Uncovering Seed vigor Responsive miRNA in hybrid Wheat and its parents by Deep Sequencing

Yue,

Liu,

Yuan

et al. 2024

Preprint

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Background: Two-line hybrid wheat technology system is one way to harness wheat heterosis both domestically and internationally. Seed vigor is a crucial parameter for assessing seed quality, as enhanced seed vigor can lead to yield increments of over 20% to a certain extent. MicroRNAs (miRNAs) were known to participate in the development and vigor of seed in plants, but its impact on seed vigor in two-line hybrid wheat remains poorly elucidated. Results: The hybrid (BS1453/11GF5135) wheat exhibited superiority in seed vigor and anti-aging capacity, compared to its male parent (11GF5135, MP) and female parent (BS1453, FP). We identified four miRNAs associated with seed vigor, all of which are novel miRNAs. The majority of targets of miRNAs were related to ubiquitin ligases, kinases, sucrose synthases and hydrolases, involving in starch and sucrose metabolism, hydrolysis, catalysis, plant hormone signal transduction, and other pathways, which played crucial roles in seed development. Additionally, we also found miR531 was differentially expressed in both male parent and hybrid, and its target interact with dihydrolipoamide acetyltransferase (E2) and dihydrolipoyl dehydrogenase (E3). Finally, We established a presumptive interaction model to speculate the relationshipof miR531 and seed vigor. Conclusions: This study analyzed the seed vigor of two-line hybrid wheat, and screened seed vigor-related miRNAs. Meanwhile speculated the genetic relationship of hybrid and parents, in terms of miRNAs. Consequently, the present study provides new insights into the miRNA-mediated gene and protein interaction network that regulates seed vigor. These findings hold significance for enhancing the yield and quality of two-line hybrid wheat, facilitating its future applications.

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Section: Hybrid Wheat Showed Heterosis In Seed Vigormentioning

confidence: 99%

Uncovering Seed vigor Responsive miRNA in hybrid Wheat and its parents by Deep Sequencing

Yue,

Liu,

Yuan

et al. 2024

Preprint

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“…The function of some of the miRNAs which are regulated by different stresses is functionally conserved across plant species. These miRNAs mediate the responses by modulating the amount of themselves, the number of mRNA targets, or the activity/mode of action of miRNA-protein complexes (Ferdous et al 2015;Swapna and Kumar 2017). In plants, the drought responsiveness of miRNAs is species dependent.…”

Section: Role Of Micrornamentioning

confidence: 99%

“…Viswanathan et al (2014) identified the miRNA of Sugarcane Streak Mosaic Virus (SCSMV) and its potential target gene in the sugarcane host plant. Several miRNAs have been identified in sugarcane crop and they are presumed to have a role in regulating the various metabolic processes (Swapna and Kumar 2017).…”

Section: Role Of Micrornamentioning

confidence: 99%

Current breeding and genomic approaches to enhance the cane and sugar productivity under abiotic stress conditions

et al. 2020

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Sugarcane (Saccharum spp.) crop is vulnerable to many abiotic stresses such as drought, salinity, waterlogging, cold and high temperature due to climate change. Over the past few decades new breeding and genomic approaches have been used to enhance the genotypic performance under abiotic stress conditions. In sugarcane, introgression of genes from wild species and allied genera for abiotic stress tolerance traits plays a significant role in the development of several stress-tolerant varieties. Moreover, the genomics and transcriptomics approaches have helped to elucidate the key genes/TFs and pathways involved in abiotic stress tolerance in sugarcane. Several novel miRNAs families /proteins or regulatory elements that are responsible for drought, salinity, and cold tolerance have been identified through high-throughput sequencing. The existing sugarcane monoploid genome sequence information opens new gateways and opportunities for researchers to improve the desired traits through efficient genome editing tools, such as the clustered regularly interspaced short palindromic repeat-Cas (CRISPR/Cas) system. TALEN mediated mutations in a highly conserved region of the caffeic acid O-methyltransferase (COMT) of sugarcane significantly reduces the lignin content in the cell wall which is amenable for biofuel production from lignocellulosic biomass. In this review, we focus on current breeding with genomic approaches and their substantial role in enhancing cane production under the abiotic stress conditions, which is expected to provide new insights to plant breeders and biotechnologists to modify their strategy in developing stress-tolerant sugarcane varieties, which can highlight the future demand of cane, bio-energy, and viability of sugar industries.

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“…RNAi-based amiRNA construct has been deployed in research to create antiviral resistance in plants against plant viral species such as tomato [25,26], cucumber [27], rice [28] and cotton [29]. Mature miRNAs in sugarcane genome have been predicted, identified, isolated, analyzed, and validated for evaluation of host-virus interactions, gene regulation and was associated with abiotic and biotic stresses [30][31][32][33][34][35][36][37][38][39][40]. Recently, experimental validation of 35 conserved mature sugarcane genome-encoded, high-confidence sof-miRNAs/ssp-miRNAs and further deposition in the miRBase database were reported.…”

Section: Introductionmentioning

confidence: 99%

The In Silico Sugarcane Genome-Encoded MicroRNA and Target Network Prediction for Targeting the SCMV

Ashraf¹,

Feng²,

Ghaffar³

et al. 2023

Preprint

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Sugarcane mosaic virus (SCMV) is a deleterious pathogen which causes widespread Sugarcane mosaic disease (SCMD) and is classified in the genus Potyvirus (Potyviridae), disseminated by the aphid vector. RNA interference (RNAi)-mediated antiviral innate immunity is a key biological process and antiviral defence system to interfere with viral genomes for controlling plant pathogens. The current study aims to analyze sugarcane (Saccharum officinarum L. and Saccharum spp.) locus-derived microRNAs (sof-miRNAs/ssp-miRNAs) with predicted potential for targeting the SCMV +ssRNA-encoded mRNAs, using ‘five algorithms’ approach. The ultimate goal in this research is to mobilize the in silico endogenous predicted sof-miRNAs/ssp-miRNAs to trigger RNAi catalytic pathway experimentally and generate sugarcane cultivars for evaluating potential antiviral resistance monitoring capability and capacity for SCMV. Experimentally validated mature sugarcane (S. officinarum, 2n = 8X = 80) and (S. spp., 2n = 100-120) sof-miRNAs/ssp-miRNAs (n = 28) were acquired for alignment with the SCMV genome. Of the 28 targeting mature locus-derived sof-miRNAs/ssp-miRNAs investigated, one sugarcane miRNA homolog, sof-miR159c, was concluded to localize potential binding site at genomic nucleotide site 3847 targeting CL ORF of SCMV. In order to validate target prediction accuracy, whether the sugarcane sof-miRNA/ssp-miRNA might bind predicted SCMV mRNA target(s), we created an integrated Circos plot. Genome-wide in-silico-predicted miRNA-mediated target gene regulatory network validated interactions that warrant in vivo analysis. The current work provides valuable evidence and biological material for generating SCMV-resistant sugarcane varieties.

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MicroRNAs and Their Regulatory Role in Sugarcane

Cited by 18 publications

References 39 publications

Uncovering Seed vigor Responsive miRNA in hybrid Wheat and its parents by Deep Sequencing

Uncovering Seed vigor Responsive miRNA in hybrid Wheat and its parents by Deep Sequencing

Current breeding and genomic approaches to enhance the cane and sugar productivity under abiotic stress conditions

The In Silico Sugarcane Genome-Encoded MicroRNA and Target Network Prediction for Targeting the SCMV

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