MicroRNAs and Cell Cycle Regulation

Carleton, Michael; Cleary, Michele A.; Linsley, Peter S.

doi:10.4161/cc.6.17.4641

Cited by 320 publications

(213 citation statements)

References 64 publications

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“…In this study, we determined that miR200c could inhibit cell growth and tumorigenesis in RCC. Dysregulation of the cell cycle leads to abnormal cell growth and tumorigenesis (39,40). Our results showed that miR-200c could inhibit cell growth by causing a G 0 -G 1 cell-cycle arrest and block entry into S-phase in RCC cells.…”

Section: Discussionmentioning

confidence: 68%

miR-200c Targets CDK2 and Suppresses Tumorigenesis in Renal Cell Carcinoma

Wang

Chen

Wang

et al. 2015

Molecular Cancer Research

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miRNA expression profiles are widely investigated in the major cancers, but their specific roles and functions in cancers have not yet to be fully elucidated. In this study, miRNA expression profiles were determined in clear cell renal cell carcinomas (ccRCC) and in matched normal kidney tissues by using a miRNA microarray platform which covers a total of 851 human miRNAs. Differential expression of 74 miRNAs were identified between ccRCC specimens and their matched adjacent noncancerous tissues, of which 30 were significantly upregulated in ccRCCs, and the other 44 were downregulated (fold change ! 2, P < 0.05). Interestingly, miR-200c was commonly downregulated in ccRCC specimens and ccRCC cell lines with significant functional consequences. Growth curve and FACS assay indicated that overexpression of miR-200c suppressed cell growth and induced cell-cycle arrest at G 0 -G 1 phases in SN12-PM6 and 786-O cells. Furthermore, miR-200c could suppress in vivo tumor growth of SN12-PM6 cells in mice. Bioinformatics exposed cyclin-dependent kinase 2 (CDK2) as a potential target of miR-200c, which was validated using a luciferase reporter assay. Mechanistic investigations revealed that miR-200c was directly responsible for suppressing the expression of CDK2 in ccRCC cell lines and xenografts. Taken together, miR-200c plays an antioncogenic role in ccRCC, through controlling cell growth and cell-cycle progression by downregulating the G 1 -S regulator CDK2.Implications: miR-200c exerts its novel antioncogenic function in renal cell carcinoma by controlling CDK2-dependent cell growth and cell-cycle progression.

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Section: Discussionmentioning

confidence: 68%

miR-200c Targets CDK2 and Suppresses Tumorigenesis in Renal Cell Carcinoma

Wang

Chen

Wang

et al. 2015

Molecular Cancer Research

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“…miRNAs usually regulate gene expression at a post-transcriptional level through imperfect pairing with the 3'-untranslated regions (3'-UTR) of target mRNAs, and can therefore modulate diverse biological processes, including cell differentiation, cell cycle, proliferation, apoptosis and cellular response to stress (1)(2)(3). The abnormal expression of miRNAs has been proven to be extensively involved in the pathogenesis of numerous types of diseases and cancers, including lung cancer (4), gastric cancer (5), cardiovascular disease (6), breast cancer (7), hepatocellular carcinoma (8) and lymphocytic leukemia (9).…”

Section: Introductionmentioning

confidence: 99%

Neuroprotection of microRNA in neurological disorders (Review)

Wang

Cheng

et al. 2014

Biomedical Reports

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Abstract. MicroRNAs (miRNAs) are small, endogenous, non-coding RNA molecules that function as post-transcriptional regulators of gene expression by imperfect base-pairing with the 3'-untranslated regions of their target mRNAs. Altered expression of numerous miRNAs has been shown to be extensively involved in the pathogenesis of various diseases and cancers. Additionally, the specific expression of miRNAs in the nervous system has indicated that miRNAs are critical for the occurrence and development of neurological diseases. Increasing evidence has shown that specific miRNAs target the expression of particular proteins that are significant in the disease pathogenesis. Therefore, miRNA-mediated regulation may be important in the occurrence and development of neurological diseases and may function as a novel biomarker and tool for clinical therapy. In the present study, the significance of miRNAs is reviewed in a number of neurological disorders and the possibility of their use in therapeutic interventions is evaluated.

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“…[1][2][3] This occurs through imperfect complementarity to the 3′ untranslated region (3′UTR) of target mRNAs; this partial homology recognition results in mRNAs translational inhibition and/or degradation, 4,5 finally leading to a reduction in protein expression level. 1 To date more than 1000 human microRNAs have been discovered (http://www.mirbase.…”

Section: Introductionmentioning

confidence: 99%