MicroRNAs 10a and 10b Regulate the Expression of Human Platelet Glycoprotein Ibα for Normal Megakaryopoiesis

Zhang, Zuping; Ran, Yali; Shaw, Tanner S.; Peng, Yuandong

doi:10.3390/ijms17111873

Cited by 18 publications

(10 citation statements)

References 32 publications

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“…In addition, the levels of miRNAs that had been previously shown to regulate platelet membrane proteins were measured. These include mir-10a, which regulates the expression of glycoprotein 1b (GP1b) [21], and mir-223, which controls the expression of putative G protein coupled receptor (P2Y12) [22, 23]. We also measured the level of mir-376c, which modifies the protease activated-receptor 4 (PAR4) signaling via phosphatidylcholine transfer protein (PCTP) [24].…”

Section: Resultsmentioning

confidence: 99%

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Cold storage conditions modify microRNA expressions for platelet transfusion

et al. 2019

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MicroRNAs (miRNAs) are small RNA molecules that modulate gene and protein expression in hematopoiesis. Platelets are known to contain a fully functional miRNA machinery. While platelets used for transfusion are normally stored at room temperature, recent evidence suggests more favorable effects under a cold-storage condition, including higher adhesion and aggregation properties. Thus, we sought to determine whether functional differences in platelets are associated with the differential profiling of platelet miRNA expressions. To obtain the miRNA expression profile, next-generation sequencing was performed on human platelets obtained from 10 healthy subjects. The miRNAs were quantified after being stored in three different conditions: 1) baseline (before storage), 2) stored at 22°C with agitation for 72 h, and 3) stored at 4°C for 72 h. Following the identification of miRNAs by sequencing, the results were validated at the level of mature miRNAs from 18 healthy subjects, by using quantitative polymerase chain reaction (qPCR). Differential expression was observed for 125 miRNAs that were stored at 4°C and 9 miRNAs stored at 22°C as compared to the baseline. The validation study by qPCR confirmed that storage at 4°C increased the expression levels (fold change 95% CI) of mir-20a-5p (1.87, p<0.0001), mir-10a-3p (1.88, p<0.0001), mir-16-2-3p (1.54, p<0.01), and mir-223-5p (1.38, p<0.05), compared with those of the samples stored at 22°C. These results show that miRNAs correlate with platelet quality under specific storage conditions. The data indicate that miRNAs could be potentially used as biomarkers of platelet quality.

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Section: Resultsmentioning

confidence: 99%

“…Importantly, platelet miRNAs are known to regulate platelet protein expressions [21, 22, 24]. For instance, a sequential increase in mir-326 expression during the storage period down-regulates the expression of the anti-apoptotic gene BCL-XL and results in platelet apoptosis [29].…”

Section: Discussionmentioning

confidence: 99%

Cold storage conditions modify microRNA expressions for platelet transfusion

et al. 2019

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“…These miRNAs have been identified with inactivation and other associated functions such as reactivity, hemostasis, differentiation and apoptosis. For example, platelet activation-related miRNAs have been identified with differential expression analysis including miR-10a, miR-10b, miR-24, miR-145, miR-183, miR-197, miR-21, miR-26a-1, miR-27b, miR-411, miR-423, and miR-451a [19,24,27,34,35,36,39,40].…”

Section: Micrornas As a Blood Bank Platelet Quality Measurement Toolmentioning

confidence: 99%

MicroRNAs as a Potential Quality Measurement Tool of Platelet Concentrate Stored in Blood Banks—A Review

Maués

Moreira-Nunes

Burbano

2019

Cells

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Background: Platelet concentrate (PC) is one of the main products used in a therapeutic transfusion. This blood component requires special storage at blood banks, however, even under good storage conditions, modifications or degradations may occur and are known as platelet storage lesions. Methods: This research was performed on scientific citation databases PubMed/Medline, ScienceDirect, and Web of Science, for publications containing platelet storage lesions. The results obtained mainly reveal the clinical applicability of miRNAs as biomarkers of storage injury and as useful tools for a problem affecting public and private health, the lack of PC bags in countries with few blood donors. The major studies listed in this review identified miRNAs associated with important platelet functions that are relevant in clinical practice as quality biomarkers of PC, such as miR-223, miR-126, miR-10a, miR-150, miR-16, miR-21, miR-326, miR-495, let-7b, let-7c, let-7e, miR-107, miR-10b, miR-145, miR-155, miR-17, miR-191, miR-197, miR-200b, miR-24, miR-331, miR-376. These miRNAs can be used in blood banks to identify platelet injury in PC bags. Conclusion: The studies described in this review relate the functions of miRNAs with molecular mechanisms that result in functional platelet differences, such as apoptosis. Thus, miRNA profiles can be used to measure the quality of storage PC for more than 5 days, identify bags with platelet injury, and distinguish those with functional platelets.

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“…A limitation of this study is the relatively small number of miRNAs investigated, only 20 more expressed, among which, some have been intensively reviewed by the literature [ 7 , 30 , 64 , 65 , 66 ]. Our results also provided a panel of miRNAs that have had their functions elucidated in platelets such as miR-223 [ 24 , 66 ], miR-20a [ 23 ] miR-126 [ 67 ], miR-10a/miR-10b [ 68 ], miR-326 [ 69 ], miR-150/miR-501/miR-338-5p/miR-432-5p/miR-411-5p [ 21 ], miR-570 [ 18 ], miR-495 [ 70 ], and miR-21/miR-27b [ 71 ]. For example, specific cold storage conditions caused changes in miRNA expressions, increasing levels of miR-20a/miR-10a/miR-16-2/miR-223, which correlated with the quality of platelets under specific conditions of storage [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

Computational Identification and Characterization of New microRNAs in Human Platelets Stored in a Blood Bank

2020

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Platelet concentrate (PC) transfusions are widely used to save the lives of patients who experience acute blood loss. MicroRNAs (miRNAs) comprise a class of molecules with a biological role which is relevant to the understanding of storage lesions in blood banks. We used a new approach to identify miRNAs in normal human platelet sRNA-Seq data from the GSE61856 repository. We identified a comprehensive miRNA expression profile, where we detected 20 of these transcripts potentially expressed in PCs stored for seven days, which had their expression levels analyzed with simulations of computational biology. Our results identified a new collection of miRNAs (miR-486-5p, miR-92a-3p, miR-103a-3p, miR-151a-3p, miR-181a-5p, and miR-221-3p) that showed a sensitivity expression pattern due to biological platelet changes during storage, confirmed by additional quantitative real-time polymerase chain reaction (qPCR) validation on 100 PC units from 500 healthy donors. We also identified that these miRNAs could transfer regulatory information on platelets, such as members of the let-7 family, by regulating the YOD1 gene, which is a deubiquitinating enzyme highly expressed in platelet hyperactivity. Our results also showed that the target genes of these miRNAs play important roles in signaling pathways, cell cycle, stress response, platelet activation and cancer. In summary, the miRNAs described in this study, have a promising application in transfusion medicine as potential biomarkers to also measure the quality and viability of the PC during storage in blood banks.

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MicroRNAs 10a and 10b Regulate the Expression of Human Platelet Glycoprotein Ibα for Normal Megakaryopoiesis

Cited by 18 publications

References 32 publications

Cold storage conditions modify microRNA expressions for platelet transfusion

Cold storage conditions modify microRNA expressions for platelet transfusion

MicroRNAs as a Potential Quality Measurement Tool of Platelet Concentrate Stored in Blood Banks—A Review

Computational Identification and Characterization of New microRNAs in Human Platelets Stored in a Blood Bank

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