MicroRNAome and Expression Profile of Developing Tooth Germ in Miniature Pigs

Li, Ang; Tao, Song; Wang, Fu; Liu, Dayong; Fan, Zhipeng; Zhang, Chunmei; He, Junqi; Wang, Songlin

doi:10.1371/journal.pone.0052256

Cited by 26 publications

(25 citation statements)

References 33 publications

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“…That is, miR-140, miR-31, miR-875-5p and miR-141 were expressed mainly during tooth morphogenesis identified at embryonic day 16 (E16), whereas miR-689, miR-720, miR-711 and miR-455 were prevalent at the cytodifferentiation stage (E18) [ 8 ]. A more recent study that combined both deep sequencing and microarray approaches to elucidate the miRNA expression profiles in the bud, cap, early bell and late bell stages of developing lower deciduous molars of miniature pigs identified 166 miRNAs expressed differentially across the four stages [ 11 ]. A subsequent bioinformatic prediction suggested that 18 of these miRNAs play key roles during tooth development, including let-7f, miR-128, miR-200b and miR-200c [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

“…A more recent study that combined both deep sequencing and microarray approaches to elucidate the miRNA expression profiles in the bud, cap, early bell and late bell stages of developing lower deciduous molars of miniature pigs identified 166 miRNAs expressed differentially across the four stages [ 11 ]. A subsequent bioinformatic prediction suggested that 18 of these miRNAs play key roles during tooth development, including let-7f, miR-128, miR-200b and miR-200c [ 11 ]. Two epithelial stem cell niches, located in the labial and lingual cervical loop regions, have been identified and shown to have different miRNA expression profiles [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

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Genome-wide analysis of miRNA and mRNA transcriptomes during amelogenesis

et al. 2014

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BackgroundIn the rodent incisor during amelogenesis, as ameloblast cells transition from secretory stage to maturation stage, their morphology and transcriptome profiles change dramatically. Prior whole genome transcriptome analysis has given a broad picture of the molecular activities dominating both stages of amelogenesis, but this type of analysis has not included miRNA transcript profiling. In this study, we set out to document which miRNAs and corresponding target genes change significantly as ameloblasts transition from secretory- to maturation-stage amelogenesis.ResultsTotal RNA samples from both secretory- and maturation-stage rat enamel organs were subjected to genome-wide miRNA and mRNA transcript profiling. We identified 59 miRNAs that were differentially expressed at the maturation stage relative to the secretory stage of enamel development (False Discovery Rate (FDR) < 0.05, fold change (FC) ≥ 1.8). In parallel, transcriptome profiling experiments identified 1,729 mRNA transcripts that were differentially expressed in the maturation stage compared to the secretory stage (FDR < 0.05, FC ≥1.8). Based on bioinformatics analyses, 5.8% (629 total) of these differentially expressed genes (DEGS) were highlighted as being the potential targets of 59 miRNAs that were differentially expressed in the opposite direction, in the same tissue samples. Although the number of predicted target DEGs was not higher than baseline expectations generated by examination of stably expressed miRNAs, Gene Ontology (GO) analysis showed that these 629 DEGS were enriched for ion transport, pH regulation, calcium handling, endocytotic, and apoptotic activities. Seven differentially expressed miRNAs (miR-21, miR-31, miR-488, miR-153, miR-135b, miR-135a and miR298) in secretory- and/or maturation-stage enamel organs were confirmed by in situ hybridization. Further, we used luciferase reporter assays to provide evidence that two of these differentially expressed miRNAs, miR-153 and miR-31, are potential regulators for their predicated target mRNAs, Lamp1 (miR-153) and Tfrc (miR-31).ConclusionsIn conclusion, these data indicate that miRNAs exhibit a dynamic expression pattern during the transition from secretory-stage to maturation-stage tooth enamel formation. Although they represent only one of numerous mechanisms influencing gene activities, miRNAs specific to the maturation stage could be involved in regulating several key processes of enamel maturation by influencing mRNA stability and translation.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-998) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genome-wide analysis of miRNA and mRNA transcriptomes during amelogenesis

et al. 2014

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“…Several are highly-expressed in big solid organs 1,44,[74][75][76] . The miR-200b and miR-214 are key miRNAs in tooth development 77,78 . When a miRNA is predominant, this suggests that it could have a significant role in the tissue, and that it could govern or be implicated in the major constitutive functions carried out by this tissue.…”

Section: Mirnas In Pig Adipose Tissue (Table 2)mentioning

confidence: 99%

Expression and Regulation Profile of Mature MicroRNA in the Pig: Relevance to Xenotransplantation

Song

Cooper

Cai

et al. 2017

Preprint

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The pig is an important source of meat production and provides a valuable model for certain human diseases. MicroRNA (miRNA), which is non-coding RNA and regulates gene expression at the posttranscriptional level, plays a critical role in various biological processes.Studies on identification and function of mature miRNAs in multiple pig tissues are increasing, yet the literature is limited. Therefore, we reviewed current research to determine the miRNAs expressed in specific pig tissues that are involved in carcass values (including muscle and adipocytes), reproduction (including pituitary, testis, and ovary), and development of some solid organs (e.g., brain, lung, kidney, and liver). We also discuss the possible regulating mechanisms of miRNA. Finally, as pig organs are suitable candidates for xenotransplantation, biomarkers of their miRNA in xenotransplantation were evaluated.

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“…Using custom‐designed miRNA microarray, Wang et al screened the miRNA in tooth germs collected from pig embryos in different developing stages. Bioinformatic analysis identified 18 key miRNAs including let‐7f, miR‐200c, and miR‐200b exhibited dynamical up‐regulation during tooth development (Li et al , ). Also, in human developing tooth germ, Wan et al () reported 17 miRNAs were up‐regulated during tooth development, while eight were down‐regulated; however, detailed miRNA expression profile in human dental epithelium and mesenchyme has not been reported yet.…”

Section: Role Of Mirna In Odontoblast Differentiationmentioning

confidence: 99%

The fine tuning role of microRNA–RNA interaction in odontoblast differentiation and disease

2014

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Dentinogenesis imperfecta and dentin dysplasia are two common types of genetic oral diseases resulted from the aberrant differentiation of odontoblast. Understanding the mechanisms of odontoblast differentiation is crucial for finding the diagnosis candidate genes and treatment targets for such kinds of diseases. Previous work has identified a battery of transcription factors and growth factors regulating odontoblast differentiation; however, the post-transcriptional regulating mechanisms of them are poorly studied. MicroRNAs (miRNA) are a group of non-coding RNAs widely studied in organ development, inflammation, and tumorigenesis because of its inhibitory effects on the target mRNAs. Also, miRNAs along with their binding targets form a complex competing endogenous RNA (ceRNA) network where miRNAs serve as the fine tuning balancers between their targets. Recent reports demonstrated the essential role of the miRNA pathway in dentinogenesis and the regulatory role of several specific miRNAs in the in vitro model of odontoblast differentiation. Herein, we will discuss the general roles of miRNA in diseases, the function of miRNAs during odontoblast differentiation, and finally the potential pathological mechanisms through which miRNAs cause the odontoblast-related diseases.

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MicroRNAome and Expression Profile of Developing Tooth Germ in Miniature Pigs

Cited by 26 publications

References 33 publications

Genome-wide analysis of miRNA and mRNA transcriptomes during amelogenesis

Genome-wide analysis of miRNA and mRNA transcriptomes during amelogenesis

Expression and Regulation Profile of Mature MicroRNA in the Pig: Relevance to Xenotransplantation

The fine tuning role of microRNA–RNA interaction in odontoblast differentiation and disease

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