MicroRNA156 amplifies transcription factor-associated cold stress tolerance in plant cells

Zhou, Mingjie; Tang, Wei

doi:10.1007/s00438-018-1516-4

Cited by 68 publications

(45 citation statements)

References 66 publications

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“…miR156 was reported to be induced by cold stress in Brassica rapa (Zeng et al, 2018), whereas it was downregulated by cold stress in rice (Cui et al, 2015), young spikes of wheat (Song et al, 2017), and Astragalus membranaceus (Abla et al, 2019). Overexpression of rice miR156 in Arabidopsis , pine, and rice resulted in improved cold stress tolerance by increasing cell viability and growth rate under cold stress (Zhou and Tang, 2018). The altered expression of miR156a-5p at the winter dormancy stage indicates that miR156 is not only a flowering regulator but also a cold stress responder in plants.…”

Section: Discussionmentioning

confidence: 99%

Identification and Temporal Expression Analysis of Conserved and Novel MicroRNAs in the Leaves of Winter Wheat Grown in the Field

Wei

Wang

et al. 2019

Front. Genet.

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Cold acclimation and vegetative/reproductive transition are two important evolutionary adaptive mechanisms for winter wheat surviving the freezing temperature in winter and successful seeds setting in the next year. MicroRNA (miRNA) is a class of regulatory small RNAs (sRNAs), which plays critical roles in the growth and development of plants. However, the regulation mechanism of miRNAs during cold acclimation and vegetative/reproductive transition of winter wheat is not much understood. In this study, four sRNA libraries from leaves of winter wheat grown in the field at the three-leaf stage, winter dormancy stage, spring green-up stage, and jointing stage were analyzed to identify known and novel miRNAs and to understand their potential roles in the growth and development of winter wheat. We examined miRNA expression using a high-throughput sequencing technique. A total of 373 known, 55 novel, and 27 putative novel miRNAs were identified. Ninety-one miRNAs were found to be differentially expressed at the four stages. Among them, the expression of six known and eight novel miRNAs was significantly suppressed at the winter dormancy stage, whereas the expression levels of seven known and eight novel miRNAs were induced at this stage; three known miRNAs and three novel miRNAs were significantly induced at the spring green-up stage; six known miRNAs were induced at the spring green-up stage and reached the highest expression level at the jointing stage; and 20 known miRNAs and 10 novel miRNAs were significantly induced at the jointing stage. Expression of a number of representative differentially expressed miRNAs was verified using quantitative real-time polymerase chain reaction (qRT-PCR). Potential target genes for known and novel miRNAs were predicted. Moreover, six novel target genes for four Pooideae species-specific miRNAs and two novel miRNAs were verified using the RNA ligase-mediated 5′-rapid amplification of cDNA ends (RLM-5’RACE) technique. These results indicate that miRNAs are key non-coding regulatory factors modulating the growth and development of wheat. Our study provides valuable information for in-depth understanding of the regulatory mechanism of miRNAs in cold acclimation and vegetative/reproductive transition of winter wheat grown in the field.

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Section: Discussionmentioning

confidence: 99%

Identification and Temporal Expression Analysis of Conserved and Novel MicroRNAs in the Leaves of Winter Wheat Grown in the Field

Wei

Wang

et al. 2019

Front. Genet.

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“…Among the 19 identified OsSPL s in rice (Yang et al, 2008), multiple essential and divergent developmental processes have been influenced. For example, OsSPL3 can increase cold stress tolerance (Zhou and Tang, 2018). Chen used near isogenic lines for mapping a minor QTL for heading date, qHd1 , which contained OsSPL2 (Chen et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Evolutionary Analyses Reveal Diverged Patterns of SQUAMOSA Promoter Binding Protein-Like (SPL) Gene Family in Oryza Genus

et al. 2019

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The SPL ( SQUAMOSA promoter binding protein-like) gene family is one of the plant-specific transcription factor families and controls a considerable number of biological functions, including floral development, phytohormone signaling, and toxin resistance. However, the evolutionary patterns and driving forces of SPL genes in the Oryza genus are still not well-characterized. In this study, we investigated a total of 105 SPL genes from six AA genome Oryza representative species ( O. barthii , O. glumipatula, O. nivara, O. rufipogon, O. glaberrima , and O. sativa ). Phylogenetic and motif analyses indicated that SPL proteins could be divided into two distinct lineages (I and II), and further studies showed lineage II consisted of three clades (IIA, IIB, and IIC). We found that clade I had comparable structural features with clade IIA, whereas genes in clade IIC displayed intrinsic differences, such as lower exon numbers and the presence of miR156 regulation elements. Nineteen orthologous groups of OsSPL s in Oryza were also identified, and most exons within those genes maintained constant length, whereas length of intron changed relatively. All groups were constrained by stronger purifying selection and diversified continually including alterative gene number, intron length, and miR156 regulation. Subsequently, cis -acting element analyses revealed the potential role of SPL s in wild rice, which might participate in light-responsive, phytohormone response, and plant growth and development. Our results shed light on that different evolutionary rates and duplication events might result in divergent evolutionary patterns in each lineage of SPL genes, providing a guide in exploring diverse function in the rice gene family among six closely related Oryza species.

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“…The generalist class is comprised by miRNAs previously described as reactive to different biotic and/or abiotic stress conditions in diverse plantspecies. Several studies support that the module miR156-SPLs besides exhibiting a broad response range to low temperatures in diverse plant-species (Zhou & Tang, 2019), also improves tolerance to salinity, heat and drought in Medicago sativa (Arshad, et al, 2017;Arshad, Gruber, Wall & Hannoufa, 2017;Matthews, Arshad & Hannoufa, 1 2019). Moreover, the interaction between miR396 and GRF is involved in the 2 modulation of the response to diverse biotic (Phytophthora nicotianae) and abiotic 3 (drought, salt, alkali, UV-B radiation, and osmotic unbalance) stress conditions (Gao et al, 2010;Kim et al, 2012;Casadevall et al, 2013;Chen, Luan & Zhai, 2015).…”

Section: Discussionmentioning

confidence: 97%

Exploring the miRNA-mediated response to combined stresses in melon plants

Villalba-Bermell

Marquez‐Molins

Marqués

et al. 2021

Preprint

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Climate change has been associated with a higher incidence of combined adverse environmental conditions that can promote a significant decrease in crop productivity. However, knowledge on how a combination of stresses might affect plant development is still scarce. MicroRNAs (miRNAs) have been proposed as potential targets for improving crop-productivity. Here, we have combined deep-sequencing, computational characterization of responsive miRNAs and validation of their regulatory role in a comprehensive analysis of melon response to several combinations of four stresses (cold, salinity, short day, and infection with a fungus). Twenty-two miRNA families responding to double and/or triple stresses were identified. The regulatory role of the differentially expressed miRNAs was validated by quantitative measurements of the expression of the corresponding target genes. A high proportion (ca. 60%) of these families (mainly highly conserved miRNAs targeting transcription factors) showed a non-additive response to multiple stresses in comparison with that observed under each one of the stresses individually. Among those miRNAs showing non-additive response to stress-combinations, most interactions were negative suggesting the existence of functional convergence in the miRNA-mediated response to combined stresses. Taken together, our results provide compelling evidences that the response to combined stresses cannot be easily predicted from the study individual stresses

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MicroRNA156 amplifies transcription factor-associated cold stress tolerance in plant cells

Cited by 68 publications

References 66 publications

Identification and Temporal Expression Analysis of Conserved and Novel MicroRNAs in the Leaves of Winter Wheat Grown in the Field

Identification and Temporal Expression Analysis of Conserved and Novel MicroRNAs in the Leaves of Winter Wheat Grown in the Field

Evolutionary Analyses Reveal Diverged Patterns of SQUAMOSA Promoter Binding Protein-Like (SPL) Gene Family in Oryza Genus

Exploring the miRNA-mediated response to combined stresses in melon plants

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