MicroRNA transcriptome analysis of chicken embryo fibroblast cells infected with Newcastle disease virus variants

Abstract

Variations in the pathogenicity of Newcastle disease virus (NDV), the agent causing Newcastle disease, are associated with variants of different virulence. A few studies have characterized the expression of microRNAs (miRNAs) in NDV-infected avian cells. Here, the expression of miRNAs in chicken embryo fibroblasts (CEFs) infected with Herts/33 and LaSota NDV strains (highly virulent and nonvirulent, respectively) was determined using RNA sequencing. miRNAs involved in NDV infection included 562 previously documented and 184 novel miRNAs. miRNA target genes involved transcription factors, cell apoptosis, ubiquitin-mediated proteolysis, and protein processing in the endoplasmic reticulum. Potential target genes associated with autophagy were verified by qRT-PCR. No studies have documented the miRNA profiles of CEFs infected with NDVs variants. This study adds to our knowledge of the cellular miRNAs involved in NDV infection and the complex molecular mechanisms mediating virus-host interactions. The results of this study will aid the development of strategies against the chicken virus.

Quantitative label-free proteomic analysis of excretory-secretory proteins in different developmental stages of Trichinella spiralis

“…The prediction of molecular functions and biological processes of differentially expressed ES proteins was performed by the Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) [ 33 ], and Clusters of Orthologous Groups (COG) databases. The protein‒protein interactions of all differentially expressed proteins were determined via the STRING database (version 10.1), and all interactions with a confidence score > 0.7 (high confidence) were retrieved.…”